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Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst

BACKGROUND: Hydatidosis is a serious and life-threatening disease that may lead to the death of the host if diagnosed and treated improperly. Apoptosis has been investigated as a mechanism of host innate immunity in suppressing parasites and also the survival of cysts in the human body. The present...

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Autores principales: Rahmani-Dehaghani, Maryam, Tolouei, Sepideh, Yousofi-Darani, Hossain, Ghayour-Najafabadi, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410427/
https://www.ncbi.nlm.nih.gov/pubmed/37564437
http://dx.doi.org/10.4103/abr.abr_152_22
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author Rahmani-Dehaghani, Maryam
Tolouei, Sepideh
Yousofi-Darani, Hossain
Ghayour-Najafabadi, Zahra
author_facet Rahmani-Dehaghani, Maryam
Tolouei, Sepideh
Yousofi-Darani, Hossain
Ghayour-Najafabadi, Zahra
author_sort Rahmani-Dehaghani, Maryam
collection PubMed
description BACKGROUND: Hydatidosis is a serious and life-threatening disease that may lead to the death of the host if diagnosed and treated improperly. Apoptosis has been investigated as a mechanism of host innate immunity in suppressing parasites and also the survival of cysts in the human body. The present study investigates the process and role of apoptosis caused by a host cell or parasite in hydatid cysts. MATERIALS AND METHODS: Survey cytotoxic effect and apoptotic mortality of hydatid-treated lymphocytes were investigated. Also, to determine the mechanism of apoptosis in host and parasite, the mean gene expressions of Bcl-2, Bax, Caspase 3 in hydatid-treated lymphocytes, and Fas-L gene in the laminated-germinal layer of fertile and infertile hydatid cysts were evaluated. RESULTS: The viability of fertile and infertile hydatid fluid-treated lymphocytes was significantly different compared with the control group. Flow cytometry also showed apoptotic cells. Bax mean gene expression was significantly different between fertile and infertile treated lymphocytes. However, there was no significant difference in the mean expression of Caspase 3, and Bcl-2 genes in these two groups. Although the expression of the Fas-L gene in infertile cysts was higher than in fertile cysts, the result was not significant. CONCLUSION: It seems that hydatid cyst fluid may induce apoptosis in lymphocytes so that, hydatid cysts can escape from the immune system and stay alive. On the other hand, the results represent the possible immune path of host apoptosis against the parasite as one of the important routes in infertility of hydatid cysts.
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spelling pubmed-104104272023-08-10 Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst Rahmani-Dehaghani, Maryam Tolouei, Sepideh Yousofi-Darani, Hossain Ghayour-Najafabadi, Zahra Adv Biomed Res Original Article BACKGROUND: Hydatidosis is a serious and life-threatening disease that may lead to the death of the host if diagnosed and treated improperly. Apoptosis has been investigated as a mechanism of host innate immunity in suppressing parasites and also the survival of cysts in the human body. The present study investigates the process and role of apoptosis caused by a host cell or parasite in hydatid cysts. MATERIALS AND METHODS: Survey cytotoxic effect and apoptotic mortality of hydatid-treated lymphocytes were investigated. Also, to determine the mechanism of apoptosis in host and parasite, the mean gene expressions of Bcl-2, Bax, Caspase 3 in hydatid-treated lymphocytes, and Fas-L gene in the laminated-germinal layer of fertile and infertile hydatid cysts were evaluated. RESULTS: The viability of fertile and infertile hydatid fluid-treated lymphocytes was significantly different compared with the control group. Flow cytometry also showed apoptotic cells. Bax mean gene expression was significantly different between fertile and infertile treated lymphocytes. However, there was no significant difference in the mean expression of Caspase 3, and Bcl-2 genes in these two groups. Although the expression of the Fas-L gene in infertile cysts was higher than in fertile cysts, the result was not significant. CONCLUSION: It seems that hydatid cyst fluid may induce apoptosis in lymphocytes so that, hydatid cysts can escape from the immune system and stay alive. On the other hand, the results represent the possible immune path of host apoptosis against the parasite as one of the important routes in infertility of hydatid cysts. Wolters Kluwer - Medknow 2023-06-30 /pmc/articles/PMC10410427/ /pubmed/37564437 http://dx.doi.org/10.4103/abr.abr_152_22 Text en Copyright: © 2023 Advanced Biomedical Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rahmani-Dehaghani, Maryam
Tolouei, Sepideh
Yousofi-Darani, Hossain
Ghayour-Najafabadi, Zahra
Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title_full Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title_fullStr Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title_full_unstemmed Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title_short Apoptosis as a Potential Target to Arrest and Survival of Hydatid Cyst
title_sort apoptosis as a potential target to arrest and survival of hydatid cyst
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410427/
https://www.ncbi.nlm.nih.gov/pubmed/37564437
http://dx.doi.org/10.4103/abr.abr_152_22
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