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Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410599/ https://www.ncbi.nlm.nih.gov/pubmed/37541125 http://dx.doi.org/10.1016/j.ultsonch.2023.106535 |
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author | Dukić, Josipa Košpić, Karla Kelava, Vanja Mavrić, Renata Nutrizio, Marinela Balen, Biljana Butorac, Ana Halil Öztop, Mecit Režek Jambrak, Anet |
author_facet | Dukić, Josipa Košpić, Karla Kelava, Vanja Mavrić, Renata Nutrizio, Marinela Balen, Biljana Butorac, Ana Halil Öztop, Mecit Režek Jambrak, Anet |
author_sort | Dukić, Josipa |
collection | PubMed |
description | Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/g(d.m.) with cold and 96.75 ± 4.30 mg/g(d.m)(.) with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/g(d.m)(.) with argon and 34.89 ± 1.59 mg/g(d.m.) with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products. |
format | Online Article Text |
id | pubmed-10410599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104105992023-08-10 Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge Dukić, Josipa Košpić, Karla Kelava, Vanja Mavrić, Renata Nutrizio, Marinela Balen, Biljana Butorac, Ana Halil Öztop, Mecit Režek Jambrak, Anet Ultrason Sonochem Original Research Article Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/g(d.m.) with cold and 96.75 ± 4.30 mg/g(d.m)(.) with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/g(d.m)(.) with argon and 34.89 ± 1.59 mg/g(d.m.) with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products. Elsevier 2023-07-27 /pmc/articles/PMC10410599/ /pubmed/37541125 http://dx.doi.org/10.1016/j.ultsonch.2023.106535 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Research Article Dukić, Josipa Košpić, Karla Kelava, Vanja Mavrić, Renata Nutrizio, Marinela Balen, Biljana Butorac, Ana Halil Öztop, Mecit Režek Jambrak, Anet Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title_full | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title_fullStr | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title_full_unstemmed | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title_short | Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge |
title_sort | alternative methods for rubisco extraction from sugar beet waste: a comparative approach of ultrasound and high voltage electrical discharge |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410599/ https://www.ncbi.nlm.nih.gov/pubmed/37541125 http://dx.doi.org/10.1016/j.ultsonch.2023.106535 |
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