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Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge

Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for to...

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Autores principales: Dukić, Josipa, Košpić, Karla, Kelava, Vanja, Mavrić, Renata, Nutrizio, Marinela, Balen, Biljana, Butorac, Ana, Halil Öztop, Mecit, Režek Jambrak, Anet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410599/
https://www.ncbi.nlm.nih.gov/pubmed/37541125
http://dx.doi.org/10.1016/j.ultsonch.2023.106535
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author Dukić, Josipa
Košpić, Karla
Kelava, Vanja
Mavrić, Renata
Nutrizio, Marinela
Balen, Biljana
Butorac, Ana
Halil Öztop, Mecit
Režek Jambrak, Anet
author_facet Dukić, Josipa
Košpić, Karla
Kelava, Vanja
Mavrić, Renata
Nutrizio, Marinela
Balen, Biljana
Butorac, Ana
Halil Öztop, Mecit
Režek Jambrak, Anet
author_sort Dukić, Josipa
collection PubMed
description Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/g(d.m.) with cold and 96.75 ± 4.30 mg/g(d.m)(.) with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/g(d.m)(.) with argon and 34.89 ± 1.59 mg/g(d.m.) with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products.
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spelling pubmed-104105992023-08-10 Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge Dukić, Josipa Košpić, Karla Kelava, Vanja Mavrić, Renata Nutrizio, Marinela Balen, Biljana Butorac, Ana Halil Öztop, Mecit Režek Jambrak, Anet Ultrason Sonochem Original Research Article Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/g(d.m.) with cold and 96.75 ± 4.30 mg/g(d.m)(.) with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/g(d.m)(.) with argon and 34.89 ± 1.59 mg/g(d.m.) with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products. Elsevier 2023-07-27 /pmc/articles/PMC10410599/ /pubmed/37541125 http://dx.doi.org/10.1016/j.ultsonch.2023.106535 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Research Article
Dukić, Josipa
Košpić, Karla
Kelava, Vanja
Mavrić, Renata
Nutrizio, Marinela
Balen, Biljana
Butorac, Ana
Halil Öztop, Mecit
Režek Jambrak, Anet
Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title_full Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title_fullStr Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title_full_unstemmed Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title_short Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge
title_sort alternative methods for rubisco extraction from sugar beet waste: a comparative approach of ultrasound and high voltage electrical discharge
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410599/
https://www.ncbi.nlm.nih.gov/pubmed/37541125
http://dx.doi.org/10.1016/j.ultsonch.2023.106535
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