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A highly sensitive nanochannel device for the detection of SUMO1 peptides

SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective dr...

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Autores principales: Qin, Yue, Zhang, Xiaoyu, Song, Yanling, Zhong, Bowen, Liu, Lu, Wang, Dongdong, Zhang, Yahui, Lu, Wenqi, Zhao, Xinjia, Jia, Zhiqi, Li, Minmin, Zhang, Lihua, Qing, Guangyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411628/
https://www.ncbi.nlm.nih.gov/pubmed/37564410
http://dx.doi.org/10.1039/d3sc02140h
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author Qin, Yue
Zhang, Xiaoyu
Song, Yanling
Zhong, Bowen
Liu, Lu
Wang, Dongdong
Zhang, Yahui
Lu, Wenqi
Zhao, Xinjia
Jia, Zhiqi
Li, Minmin
Zhang, Lihua
Qing, Guangyan
author_facet Qin, Yue
Zhang, Xiaoyu
Song, Yanling
Zhong, Bowen
Liu, Lu
Wang, Dongdong
Zhang, Yahui
Lu, Wenqi
Zhao, Xinjia
Jia, Zhiqi
Li, Minmin
Zhang, Lihua
Qing, Guangyan
author_sort Qin, Yue
collection PubMed
description SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective drugs. Reliable antibodies against SUMO2/3 are commercially available; however, efficient detectors are yet to be developed for SUMO1, which has only 50% homology with SUMO2 and SUMO3. Here, using phage display technology, we identified two cyclic peptide (CP) sequences that could specifically bind to the terminal dodecapeptide sequence of SUMO1. Then we combined the CPs and polyethylene terephthalate conical nanochannel films to fabricate a nanochannel device highly sensitive towards the SUMO1 terminal peptide and protein; sensitivity was achieved by ensuring marked variations in both transmembrane ionic current and Faraday current. The satisfactory SUMO1-sensing ability of this device makes it a promising tool for the time-point monitoring of the SENP1 enzyme-catalyzed de-SUMOylation reaction and cellular imaging. This study not only solves the challenge of SUMO1 precise recognition that could promote SUMO1 proteomics analysis, but also demonstrates the good potential of the nanochannel device in monitoring of enzymes and discovery of effective drugs.
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spelling pubmed-104116282023-08-10 A highly sensitive nanochannel device for the detection of SUMO1 peptides Qin, Yue Zhang, Xiaoyu Song, Yanling Zhong, Bowen Liu, Lu Wang, Dongdong Zhang, Yahui Lu, Wenqi Zhao, Xinjia Jia, Zhiqi Li, Minmin Zhang, Lihua Qing, Guangyan Chem Sci Chemistry SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective drugs. Reliable antibodies against SUMO2/3 are commercially available; however, efficient detectors are yet to be developed for SUMO1, which has only 50% homology with SUMO2 and SUMO3. Here, using phage display technology, we identified two cyclic peptide (CP) sequences that could specifically bind to the terminal dodecapeptide sequence of SUMO1. Then we combined the CPs and polyethylene terephthalate conical nanochannel films to fabricate a nanochannel device highly sensitive towards the SUMO1 terminal peptide and protein; sensitivity was achieved by ensuring marked variations in both transmembrane ionic current and Faraday current. The satisfactory SUMO1-sensing ability of this device makes it a promising tool for the time-point monitoring of the SENP1 enzyme-catalyzed de-SUMOylation reaction and cellular imaging. This study not only solves the challenge of SUMO1 precise recognition that could promote SUMO1 proteomics analysis, but also demonstrates the good potential of the nanochannel device in monitoring of enzymes and discovery of effective drugs. The Royal Society of Chemistry 2023-07-13 /pmc/articles/PMC10411628/ /pubmed/37564410 http://dx.doi.org/10.1039/d3sc02140h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qin, Yue
Zhang, Xiaoyu
Song, Yanling
Zhong, Bowen
Liu, Lu
Wang, Dongdong
Zhang, Yahui
Lu, Wenqi
Zhao, Xinjia
Jia, Zhiqi
Li, Minmin
Zhang, Lihua
Qing, Guangyan
A highly sensitive nanochannel device for the detection of SUMO1 peptides
title A highly sensitive nanochannel device for the detection of SUMO1 peptides
title_full A highly sensitive nanochannel device for the detection of SUMO1 peptides
title_fullStr A highly sensitive nanochannel device for the detection of SUMO1 peptides
title_full_unstemmed A highly sensitive nanochannel device for the detection of SUMO1 peptides
title_short A highly sensitive nanochannel device for the detection of SUMO1 peptides
title_sort highly sensitive nanochannel device for the detection of sumo1 peptides
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411628/
https://www.ncbi.nlm.nih.gov/pubmed/37564410
http://dx.doi.org/10.1039/d3sc02140h
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