Cargando…
A highly sensitive nanochannel device for the detection of SUMO1 peptides
SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective dr...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411628/ https://www.ncbi.nlm.nih.gov/pubmed/37564410 http://dx.doi.org/10.1039/d3sc02140h |
_version_ | 1785086706712576000 |
---|---|
author | Qin, Yue Zhang, Xiaoyu Song, Yanling Zhong, Bowen Liu, Lu Wang, Dongdong Zhang, Yahui Lu, Wenqi Zhao, Xinjia Jia, Zhiqi Li, Minmin Zhang, Lihua Qing, Guangyan |
author_facet | Qin, Yue Zhang, Xiaoyu Song, Yanling Zhong, Bowen Liu, Lu Wang, Dongdong Zhang, Yahui Lu, Wenqi Zhao, Xinjia Jia, Zhiqi Li, Minmin Zhang, Lihua Qing, Guangyan |
author_sort | Qin, Yue |
collection | PubMed |
description | SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective drugs. Reliable antibodies against SUMO2/3 are commercially available; however, efficient detectors are yet to be developed for SUMO1, which has only 50% homology with SUMO2 and SUMO3. Here, using phage display technology, we identified two cyclic peptide (CP) sequences that could specifically bind to the terminal dodecapeptide sequence of SUMO1. Then we combined the CPs and polyethylene terephthalate conical nanochannel films to fabricate a nanochannel device highly sensitive towards the SUMO1 terminal peptide and protein; sensitivity was achieved by ensuring marked variations in both transmembrane ionic current and Faraday current. The satisfactory SUMO1-sensing ability of this device makes it a promising tool for the time-point monitoring of the SENP1 enzyme-catalyzed de-SUMOylation reaction and cellular imaging. This study not only solves the challenge of SUMO1 precise recognition that could promote SUMO1 proteomics analysis, but also demonstrates the good potential of the nanochannel device in monitoring of enzymes and discovery of effective drugs. |
format | Online Article Text |
id | pubmed-10411628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-104116282023-08-10 A highly sensitive nanochannel device for the detection of SUMO1 peptides Qin, Yue Zhang, Xiaoyu Song, Yanling Zhong, Bowen Liu, Lu Wang, Dongdong Zhang, Yahui Lu, Wenqi Zhao, Xinjia Jia, Zhiqi Li, Minmin Zhang, Lihua Qing, Guangyan Chem Sci Chemistry SUMOylation is an important and highly dynamic post-translational modification (PTM) process of protein, and its disequilibrium may cause various diseases, such as cancers and neurodegenerative disorders. SUMO proteins must be accurately detected to understand disease states and develop effective drugs. Reliable antibodies against SUMO2/3 are commercially available; however, efficient detectors are yet to be developed for SUMO1, which has only 50% homology with SUMO2 and SUMO3. Here, using phage display technology, we identified two cyclic peptide (CP) sequences that could specifically bind to the terminal dodecapeptide sequence of SUMO1. Then we combined the CPs and polyethylene terephthalate conical nanochannel films to fabricate a nanochannel device highly sensitive towards the SUMO1 terminal peptide and protein; sensitivity was achieved by ensuring marked variations in both transmembrane ionic current and Faraday current. The satisfactory SUMO1-sensing ability of this device makes it a promising tool for the time-point monitoring of the SENP1 enzyme-catalyzed de-SUMOylation reaction and cellular imaging. This study not only solves the challenge of SUMO1 precise recognition that could promote SUMO1 proteomics analysis, but also demonstrates the good potential of the nanochannel device in monitoring of enzymes and discovery of effective drugs. The Royal Society of Chemistry 2023-07-13 /pmc/articles/PMC10411628/ /pubmed/37564410 http://dx.doi.org/10.1039/d3sc02140h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Qin, Yue Zhang, Xiaoyu Song, Yanling Zhong, Bowen Liu, Lu Wang, Dongdong Zhang, Yahui Lu, Wenqi Zhao, Xinjia Jia, Zhiqi Li, Minmin Zhang, Lihua Qing, Guangyan A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title | A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title_full | A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title_fullStr | A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title_full_unstemmed | A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title_short | A highly sensitive nanochannel device for the detection of SUMO1 peptides |
title_sort | highly sensitive nanochannel device for the detection of sumo1 peptides |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411628/ https://www.ncbi.nlm.nih.gov/pubmed/37564410 http://dx.doi.org/10.1039/d3sc02140h |
work_keys_str_mv | AT qinyue ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhangxiaoyu ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT songyanling ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhongbowen ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT liulu ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT wangdongdong ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhangyahui ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT luwenqi ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhaoxinjia ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT jiazhiqi ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT liminmin ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhanglihua ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT qingguangyan ahighlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT qinyue highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhangxiaoyu highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT songyanling highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhongbowen highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT liulu highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT wangdongdong highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhangyahui highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT luwenqi highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhaoxinjia highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT jiazhiqi highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT liminmin highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT zhanglihua highlysensitivenanochanneldeviceforthedetectionofsumo1peptides AT qingguangyan highlysensitivenanochanneldeviceforthedetectionofsumo1peptides |