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Brain rhythms control microglial response and cytokine expression via NF-κB signaling

Microglia transform in response to changes in sensory or neural activity, such as sensory deprivation. However, little is known about how specific frequencies of neural activity, or brain rhythms, affect microglia and cytokine signaling. Using visual noninvasive flickering sensory stimulation (flick...

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Autores principales: Prichard, Ashley, Garza, Kristie M., Shridhar, Avni, He, Christopher, Bitarafan, Sara, Pybus, Alyssa, Wang, Yunmiao, Snyder, Emma, Goodson, Matthew C., Franklin, Tina C., Jaeger, Dieter, Wood, Levi B., Singer, Annabelle C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411883/
https://www.ncbi.nlm.nih.gov/pubmed/37556553
http://dx.doi.org/10.1126/sciadv.adf5672
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author Prichard, Ashley
Garza, Kristie M.
Shridhar, Avni
He, Christopher
Bitarafan, Sara
Pybus, Alyssa
Wang, Yunmiao
Snyder, Emma
Goodson, Matthew C.
Franklin, Tina C.
Jaeger, Dieter
Wood, Levi B.
Singer, Annabelle C.
author_facet Prichard, Ashley
Garza, Kristie M.
Shridhar, Avni
He, Christopher
Bitarafan, Sara
Pybus, Alyssa
Wang, Yunmiao
Snyder, Emma
Goodson, Matthew C.
Franklin, Tina C.
Jaeger, Dieter
Wood, Levi B.
Singer, Annabelle C.
author_sort Prichard, Ashley
collection PubMed
description Microglia transform in response to changes in sensory or neural activity, such as sensory deprivation. However, little is known about how specific frequencies of neural activity, or brain rhythms, affect microglia and cytokine signaling. Using visual noninvasive flickering sensory stimulation (flicker) to induce electrical neural activity at 40 hertz, within the gamma band, and 20 hertz, within the beta band, we found that these brain rhythms differentially affect microglial morphology and cytokine expression in healthy animals. Flicker induced expression of certain cytokines independently of microglia, including interleukin-10 and macrophage colony-stimulating factor. We hypothesized that nuclear factor κB (NF-κB) plays a causal role in frequency-specific cytokine and microglial responses because this pathway is activated by synaptic activity and regulates cytokines. After flicker, phospho–NF-κB colabeled with neurons more than microglia. Inhibition of NF-κB signaling down-regulated flicker-induced cytokine expression and attenuated flicker-induced changes in microglial morphology. These results reveal a mechanism through which brain rhythms affect brain function by altering microglial morphology and cytokines via NF-κB.
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spelling pubmed-104118832023-08-10 Brain rhythms control microglial response and cytokine expression via NF-κB signaling Prichard, Ashley Garza, Kristie M. Shridhar, Avni He, Christopher Bitarafan, Sara Pybus, Alyssa Wang, Yunmiao Snyder, Emma Goodson, Matthew C. Franklin, Tina C. Jaeger, Dieter Wood, Levi B. Singer, Annabelle C. Sci Adv Neuroscience Microglia transform in response to changes in sensory or neural activity, such as sensory deprivation. However, little is known about how specific frequencies of neural activity, or brain rhythms, affect microglia and cytokine signaling. Using visual noninvasive flickering sensory stimulation (flicker) to induce electrical neural activity at 40 hertz, within the gamma band, and 20 hertz, within the beta band, we found that these brain rhythms differentially affect microglial morphology and cytokine expression in healthy animals. Flicker induced expression of certain cytokines independently of microglia, including interleukin-10 and macrophage colony-stimulating factor. We hypothesized that nuclear factor κB (NF-κB) plays a causal role in frequency-specific cytokine and microglial responses because this pathway is activated by synaptic activity and regulates cytokines. After flicker, phospho–NF-κB colabeled with neurons more than microglia. Inhibition of NF-κB signaling down-regulated flicker-induced cytokine expression and attenuated flicker-induced changes in microglial morphology. These results reveal a mechanism through which brain rhythms affect brain function by altering microglial morphology and cytokines via NF-κB. American Association for the Advancement of Science 2023-08-09 /pmc/articles/PMC10411883/ /pubmed/37556553 http://dx.doi.org/10.1126/sciadv.adf5672 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Neuroscience
Prichard, Ashley
Garza, Kristie M.
Shridhar, Avni
He, Christopher
Bitarafan, Sara
Pybus, Alyssa
Wang, Yunmiao
Snyder, Emma
Goodson, Matthew C.
Franklin, Tina C.
Jaeger, Dieter
Wood, Levi B.
Singer, Annabelle C.
Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title_full Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title_fullStr Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title_full_unstemmed Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title_short Brain rhythms control microglial response and cytokine expression via NF-κB signaling
title_sort brain rhythms control microglial response and cytokine expression via nf-κb signaling
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411883/
https://www.ncbi.nlm.nih.gov/pubmed/37556553
http://dx.doi.org/10.1126/sciadv.adf5672
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