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Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411996/ https://www.ncbi.nlm.nih.gov/pubmed/37564277 http://dx.doi.org/10.1063/5.0155207 |
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author | Bertucci, Taylor Kakarla, Shravani Winkelman, Max A. Lane, Keith Stevens, Katherine Lotz, Steven Grath, Alexander James, Daylon Temple, Sally Dai, Guohao |
author_facet | Bertucci, Taylor Kakarla, Shravani Winkelman, Max A. Lane, Keith Stevens, Katherine Lotz, Steven Grath, Alexander James, Daylon Temple, Sally Dai, Guohao |
author_sort | Bertucci, Taylor |
collection | PubMed |
description | During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro. |
format | Online Article Text |
id | pubmed-10411996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-104119962023-08-10 Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells Bertucci, Taylor Kakarla, Shravani Winkelman, Max A. Lane, Keith Stevens, Katherine Lotz, Steven Grath, Alexander James, Daylon Temple, Sally Dai, Guohao APL Bioeng Articles During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro. AIP Publishing LLC 2023-08-08 /pmc/articles/PMC10411996/ /pubmed/37564277 http://dx.doi.org/10.1063/5.0155207 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Bertucci, Taylor Kakarla, Shravani Winkelman, Max A. Lane, Keith Stevens, Katherine Lotz, Steven Grath, Alexander James, Daylon Temple, Sally Dai, Guohao Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title | Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title_full | Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title_fullStr | Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title_full_unstemmed | Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title_short | Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
title_sort | direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411996/ https://www.ncbi.nlm.nih.gov/pubmed/37564277 http://dx.doi.org/10.1063/5.0155207 |
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