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Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells

During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blo...

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Autores principales: Bertucci, Taylor, Kakarla, Shravani, Winkelman, Max A., Lane, Keith, Stevens, Katherine, Lotz, Steven, Grath, Alexander, James, Daylon, Temple, Sally, Dai, Guohao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411996/
https://www.ncbi.nlm.nih.gov/pubmed/37564277
http://dx.doi.org/10.1063/5.0155207
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author Bertucci, Taylor
Kakarla, Shravani
Winkelman, Max A.
Lane, Keith
Stevens, Katherine
Lotz, Steven
Grath, Alexander
James, Daylon
Temple, Sally
Dai, Guohao
author_facet Bertucci, Taylor
Kakarla, Shravani
Winkelman, Max A.
Lane, Keith
Stevens, Katherine
Lotz, Steven
Grath, Alexander
James, Daylon
Temple, Sally
Dai, Guohao
author_sort Bertucci, Taylor
collection PubMed
description During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro.
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spelling pubmed-104119962023-08-10 Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells Bertucci, Taylor Kakarla, Shravani Winkelman, Max A. Lane, Keith Stevens, Katherine Lotz, Steven Grath, Alexander James, Daylon Temple, Sally Dai, Guohao APL Bioeng Articles During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro. AIP Publishing LLC 2023-08-08 /pmc/articles/PMC10411996/ /pubmed/37564277 http://dx.doi.org/10.1063/5.0155207 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Articles
Bertucci, Taylor
Kakarla, Shravani
Winkelman, Max A.
Lane, Keith
Stevens, Katherine
Lotz, Steven
Grath, Alexander
James, Daylon
Temple, Sally
Dai, Guohao
Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title_full Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title_fullStr Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title_full_unstemmed Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title_short Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
title_sort direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411996/
https://www.ncbi.nlm.nih.gov/pubmed/37564277
http://dx.doi.org/10.1063/5.0155207
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