Cargando…

Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis

BACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally. Circular RNAs (circRNAs), as a type of noncoding RNAs, reportedly participate in various tumor biological processes. However, the role of circHDAC1_004 in HCC remains unclear. Thus, we aimed to ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Bin, Jia, Wenbo, Feng, Yanzhi, Wang, Jinyi, Wang, Jing, Zhu, Deming, Xu, Chao, Liang, Litao, Ding, Wenzhou, Zhou, Yongping, Kong, Lianbao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: XIA & HE Publishing Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412708/
https://www.ncbi.nlm.nih.gov/pubmed/37577235
http://dx.doi.org/10.14218/JCTH.2022.00097
_version_ 1785086972675489792
author Xu, Bin
Jia, Wenbo
Feng, Yanzhi
Wang, Jinyi
Wang, Jing
Zhu, Deming
Xu, Chao
Liang, Litao
Ding, Wenzhou
Zhou, Yongping
Kong, Lianbao
author_facet Xu, Bin
Jia, Wenbo
Feng, Yanzhi
Wang, Jinyi
Wang, Jing
Zhu, Deming
Xu, Chao
Liang, Litao
Ding, Wenzhou
Zhou, Yongping
Kong, Lianbao
author_sort Xu, Bin
collection PubMed
description BACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally. Circular RNAs (circRNAs), as a type of noncoding RNAs, reportedly participate in various tumor biological processes. However, the role of circHDAC1_004 in HCC remains unclear. Thus, we aimed to explore the role and the underlying mechanisms of circHDAC1_004 in the development and progression of HCC. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect circHDAC1_004 expression (circ_0005339) in HCC. Sanger sequencing and agarose gel electrophoresis were used to determine the structure of circHDAC1_004. In vitro and in vivo experiments were used to determine the biological function of circHDAC1_004 in HCC. Herein, qRT-PCR, RNA immunoprecipitation, western blotting, and a luciferase reporter assay were used to explore the relationships among circHDAC1_004, miR-361-3p, and NACC1. RESULTS: circHDAC1_004 was upregulated in HCC and significantly associated with poor overall survival. circHDAC1_004 promoted HCC cell proliferation, stemness, migration, and invasion. In addition, circHDAC1_004 upregulated human umbilical vein endothelial cells (HUVECs) and promoted angiogenesis through exosomes. circHDAC1_004 promoted NACC1 expression and stimulated the epithelial-mesenchymal transition pathway by sponging miR-361-3p. CONCLUSIONS: We found that circHDAC1_004 overexpression enhanced the proliferation, stemness, and metastasis of HCC via the miR-361-3p/NACC1 axis and promoted HCC angiogenesis through exosomes. Our findings may help develop a possible therapeutic strategy for HCC.
format Online
Article
Text
id pubmed-10412708
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher XIA & HE Publishing Inc.
record_format MEDLINE/PubMed
spelling pubmed-104127082023-08-11 Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis Xu, Bin Jia, Wenbo Feng, Yanzhi Wang, Jinyi Wang, Jing Zhu, Deming Xu, Chao Liang, Litao Ding, Wenzhou Zhou, Yongping Kong, Lianbao J Clin Transl Hepatol Original Article BACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is among the most common malignant tumors globally. Circular RNAs (circRNAs), as a type of noncoding RNAs, reportedly participate in various tumor biological processes. However, the role of circHDAC1_004 in HCC remains unclear. Thus, we aimed to explore the role and the underlying mechanisms of circHDAC1_004 in the development and progression of HCC. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect circHDAC1_004 expression (circ_0005339) in HCC. Sanger sequencing and agarose gel electrophoresis were used to determine the structure of circHDAC1_004. In vitro and in vivo experiments were used to determine the biological function of circHDAC1_004 in HCC. Herein, qRT-PCR, RNA immunoprecipitation, western blotting, and a luciferase reporter assay were used to explore the relationships among circHDAC1_004, miR-361-3p, and NACC1. RESULTS: circHDAC1_004 was upregulated in HCC and significantly associated with poor overall survival. circHDAC1_004 promoted HCC cell proliferation, stemness, migration, and invasion. In addition, circHDAC1_004 upregulated human umbilical vein endothelial cells (HUVECs) and promoted angiogenesis through exosomes. circHDAC1_004 promoted NACC1 expression and stimulated the epithelial-mesenchymal transition pathway by sponging miR-361-3p. CONCLUSIONS: We found that circHDAC1_004 overexpression enhanced the proliferation, stemness, and metastasis of HCC via the miR-361-3p/NACC1 axis and promoted HCC angiogenesis through exosomes. Our findings may help develop a possible therapeutic strategy for HCC. XIA & HE Publishing Inc. 2023-10-28 2023-03-22 /pmc/articles/PMC10412708/ /pubmed/37577235 http://dx.doi.org/10.14218/JCTH.2022.00097 Text en © 2023 Authors. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 International License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Xu, Bin
Jia, Wenbo
Feng, Yanzhi
Wang, Jinyi
Wang, Jing
Zhu, Deming
Xu, Chao
Liang, Litao
Ding, Wenzhou
Zhou, Yongping
Kong, Lianbao
Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title_full Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title_fullStr Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title_full_unstemmed Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title_short Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis
title_sort exosome-transported circhdac1_004 promotes proliferation, migration, and angiogenesis of hepatocellular carcinoma by the mir-361-3p/nacc1 axis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412708/
https://www.ncbi.nlm.nih.gov/pubmed/37577235
http://dx.doi.org/10.14218/JCTH.2022.00097
work_keys_str_mv AT xubin exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT jiawenbo exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT fengyanzhi exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT wangjinyi exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT wangjing exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT zhudeming exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT xuchao exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT lianglitao exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT dingwenzhou exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT zhouyongping exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis
AT konglianbao exosometransportedcirchdac1004promotesproliferationmigrationandangiogenesisofhepatocellularcarcinomabythemir3613pnacc1axis