Cargando…

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead

With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signa...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, De-long, Guo, Yan, Ma, Bing-chan, Lin, Yong-qin, Wang, Hai-jun, Gao, Chao-xian, Liu, Ming-qi, Zhang, Nai-xing, Luo, Hao, Hui, Chang-ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413148/
https://www.ncbi.nlm.nih.gov/pubmed/37577420
http://dx.doi.org/10.3389/fmicb.2023.1218933
_version_ 1785087073310474240
author Zhu, De-long
Guo, Yan
Ma, Bing-chan
Lin, Yong-qin
Wang, Hai-jun
Gao, Chao-xian
Liu, Ming-qi
Zhang, Nai-xing
Luo, Hao
Hui, Chang-ye
author_facet Zhu, De-long
Guo, Yan
Ma, Bing-chan
Lin, Yong-qin
Wang, Hai-jun
Gao, Chao-xian
Liu, Ming-qi
Zhang, Nai-xing
Luo, Hao
Hui, Chang-ye
author_sort Zhu, De-long
collection PubMed
description With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue. The pbr operon from Klebsiella pneumoniae is selectively induced by bioavailable Pb(II). In the present study, the proviolacein biosynthetic gene cluster was transcriptionally fused to the pbr Pb(II) responsive element and introduced into Escherichia coli. The resultant biosensor responded to Pb(II) in a time- and dose-dependent manner. After a 5-h incubation with Pb(II), the brown pigment was produced, which could be extracted into n-butanol. Extra hydrogen peroxide treatment during n-butanol extract resulted in the generation of a stable green pigment. An increased brown signal was observed upon exposure to lead concentrations above 2.93 nM, and a linear regression was fitted from 2.93 to 3,000 nM. Extra oxidation significantly decreased the difference between parallel groups. The green signal responded to as low as 0.183 nM Pb(II), and a non-linear regression was fitted in a wide concentration range from 0.183 to 3,000 nM. The specific response toward Pb(II) was not interfered with by various metals except for Cd(II) and Hg(II). The PV-based biosensor was validated in monitoring bioaccessible Pb(II) spiked into environmental water. The complex matrices did not influence the regression relationship between spiked Pb(II) and the dual-color signals. Direct reading with the naked eye and colorimetric quantification enable the PV-based biosensor to be a dual-color and low-cost bioindicator for pollutant heavy metal.
format Online
Article
Text
id pubmed-10413148
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104131482023-08-11 Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead Zhu, De-long Guo, Yan Ma, Bing-chan Lin, Yong-qin Wang, Hai-jun Gao, Chao-xian Liu, Ming-qi Zhang, Nai-xing Luo, Hao Hui, Chang-ye Front Microbiol Microbiology With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue. The pbr operon from Klebsiella pneumoniae is selectively induced by bioavailable Pb(II). In the present study, the proviolacein biosynthetic gene cluster was transcriptionally fused to the pbr Pb(II) responsive element and introduced into Escherichia coli. The resultant biosensor responded to Pb(II) in a time- and dose-dependent manner. After a 5-h incubation with Pb(II), the brown pigment was produced, which could be extracted into n-butanol. Extra hydrogen peroxide treatment during n-butanol extract resulted in the generation of a stable green pigment. An increased brown signal was observed upon exposure to lead concentrations above 2.93 nM, and a linear regression was fitted from 2.93 to 3,000 nM. Extra oxidation significantly decreased the difference between parallel groups. The green signal responded to as low as 0.183 nM Pb(II), and a non-linear regression was fitted in a wide concentration range from 0.183 to 3,000 nM. The specific response toward Pb(II) was not interfered with by various metals except for Cd(II) and Hg(II). The PV-based biosensor was validated in monitoring bioaccessible Pb(II) spiked into environmental water. The complex matrices did not influence the regression relationship between spiked Pb(II) and the dual-color signals. Direct reading with the naked eye and colorimetric quantification enable the PV-based biosensor to be a dual-color and low-cost bioindicator for pollutant heavy metal. Frontiers Media S.A. 2023-07-27 /pmc/articles/PMC10413148/ /pubmed/37577420 http://dx.doi.org/10.3389/fmicb.2023.1218933 Text en Copyright © 2023 Zhu, Guo, Ma, Lin, Wang, Gao, Liu, Zhang, Luo and Hui. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhu, De-long
Guo, Yan
Ma, Bing-chan
Lin, Yong-qin
Wang, Hai-jun
Gao, Chao-xian
Liu, Ming-qi
Zhang, Nai-xing
Luo, Hao
Hui, Chang-ye
Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title_full Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title_fullStr Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title_full_unstemmed Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title_short Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
title_sort pb(ii)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413148/
https://www.ncbi.nlm.nih.gov/pubmed/37577420
http://dx.doi.org/10.3389/fmicb.2023.1218933
work_keys_str_mv AT zhudelong pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT guoyan pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT mabingchan pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT linyongqin pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT wanghaijun pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT gaochaoxian pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT liumingqi pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT zhangnaixing pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT luohao pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead
AT huichangye pbiiinducibleproviolaceinbiosynthesisenablesadualcolorbiosensortowardenvironmentallead