Cargando…

Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma

Liver cancer is among the top leading causes of cancer mortality worldwide. Particularly, hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (CCA) have been extensively investigated from the aspect of tumor biology. However, a comprehensive and systematic understanding of the molecul...

Descripción completa

Detalles Bibliográficos
Autores principales: Yi, Xiao, Zhu, Jiang, Liu, Wei, Peng, Li, Lu, Cong, Sun, Ping, Huang, Lingling, Nie, Xiu, Huang, Shi'ang, Guo, Tiannan, Zhu, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413158/
https://www.ncbi.nlm.nih.gov/pubmed/37353004
http://dx.doi.org/10.1016/j.mcpro.2023.100604
_version_ 1785087075734781952
author Yi, Xiao
Zhu, Jiang
Liu, Wei
Peng, Li
Lu, Cong
Sun, Ping
Huang, Lingling
Nie, Xiu
Huang, Shi'ang
Guo, Tiannan
Zhu, Yi
author_facet Yi, Xiao
Zhu, Jiang
Liu, Wei
Peng, Li
Lu, Cong
Sun, Ping
Huang, Lingling
Nie, Xiu
Huang, Shi'ang
Guo, Tiannan
Zhu, Yi
author_sort Yi, Xiao
collection PubMed
description Liver cancer is among the top leading causes of cancer mortality worldwide. Particularly, hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (CCA) have been extensively investigated from the aspect of tumor biology. However, a comprehensive and systematic understanding of the molecular characteristics of HCC and CCA remains absent. Here, we characterized the proteome landscapes of HCC and CCA using the data-independent acquisition (DIA) mass spectrometry (MS) method. By comparing the quantitative proteomes of HCC and CCA, we found several differences between the two cancer types. In particular, we found an abnormal lipid metabolism in HCC and activated extracellular matrix-related pathways in CCA. We next developed a three-protein classifier to distinguish CCA from HCC, achieving an area under the curve (AUC) of 0.92, and an accuracy of 90% in an independent validation cohort of 51 patients. The distinct molecular characteristics of HCC and CCA presented in this study provide new insights into the tumor biology of these two major important primary liver cancers. Our findings may help develop more efficient diagnostic approaches and new targeted drug treatments.
format Online
Article
Text
id pubmed-10413158
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-104131582023-08-11 Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma Yi, Xiao Zhu, Jiang Liu, Wei Peng, Li Lu, Cong Sun, Ping Huang, Lingling Nie, Xiu Huang, Shi'ang Guo, Tiannan Zhu, Yi Mol Cell Proteomics Research Liver cancer is among the top leading causes of cancer mortality worldwide. Particularly, hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (CCA) have been extensively investigated from the aspect of tumor biology. However, a comprehensive and systematic understanding of the molecular characteristics of HCC and CCA remains absent. Here, we characterized the proteome landscapes of HCC and CCA using the data-independent acquisition (DIA) mass spectrometry (MS) method. By comparing the quantitative proteomes of HCC and CCA, we found several differences between the two cancer types. In particular, we found an abnormal lipid metabolism in HCC and activated extracellular matrix-related pathways in CCA. We next developed a three-protein classifier to distinguish CCA from HCC, achieving an area under the curve (AUC) of 0.92, and an accuracy of 90% in an independent validation cohort of 51 patients. The distinct molecular characteristics of HCC and CCA presented in this study provide new insights into the tumor biology of these two major important primary liver cancers. Our findings may help develop more efficient diagnostic approaches and new targeted drug treatments. American Society for Biochemistry and Molecular Biology 2023-06-22 /pmc/articles/PMC10413158/ /pubmed/37353004 http://dx.doi.org/10.1016/j.mcpro.2023.100604 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research
Yi, Xiao
Zhu, Jiang
Liu, Wei
Peng, Li
Lu, Cong
Sun, Ping
Huang, Lingling
Nie, Xiu
Huang, Shi'ang
Guo, Tiannan
Zhu, Yi
Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title_full Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title_fullStr Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title_full_unstemmed Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title_short Proteome Landscapes of Human Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma
title_sort proteome landscapes of human hepatocellular carcinoma and intrahepatic cholangiocarcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413158/
https://www.ncbi.nlm.nih.gov/pubmed/37353004
http://dx.doi.org/10.1016/j.mcpro.2023.100604
work_keys_str_mv AT yixiao proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT zhujiang proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT liuwei proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT pengli proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT lucong proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT sunping proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT huanglingling proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT niexiu proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT huangshiang proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT guotiannan proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma
AT zhuyi proteomelandscapesofhumanhepatocellularcarcinomaandintrahepaticcholangiocarcinoma