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Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)

The actin-depolymerizing factor (ADF) encoded by a family of genes is highly conserved among eukaryotes and plays critical roles in the various processes of plant growth, development, and stress responses via the remodeling of the architecture of the actin cytoskeleton. However, the ADF family and t...

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Autores principales: Sun, Yongwang, Wang, Deying, Shi, Mengmeng, Gong, Yujie, Yin, Shuwen, Jiao, Yexuan, Guo, Shangjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413265/
https://www.ncbi.nlm.nih.gov/pubmed/37575943
http://dx.doi.org/10.3389/fpls.2023.1236175
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author Sun, Yongwang
Wang, Deying
Shi, Mengmeng
Gong, Yujie
Yin, Shuwen
Jiao, Yexuan
Guo, Shangjing
author_facet Sun, Yongwang
Wang, Deying
Shi, Mengmeng
Gong, Yujie
Yin, Shuwen
Jiao, Yexuan
Guo, Shangjing
author_sort Sun, Yongwang
collection PubMed
description The actin-depolymerizing factor (ADF) encoded by a family of genes is highly conserved among eukaryotes and plays critical roles in the various processes of plant growth, development, and stress responses via the remodeling of the architecture of the actin cytoskeleton. However, the ADF family and the encoded proteins in soybean (Glycine max) have not yet been systematically investigated. In this study, 18 GmADF genes (GmADF1 – GmADF18) were identified in the soybean genome and were mapped to 14 different chromosomes. Phylogenetic analysis classified them into four groups, which was confirmed by their structure and the distribution of conserved motifs in the encoded proteins. Additionally, 29 paralogous gene pairs were identified in the GmADF family, and analysis of their Ka/Ks ratios indicated their purity-based selection during the evolutionary expansion of the soybean genome. The analysis of the expression profiles based on the RNA-seq and qRT-PCR data indicated that GmADFs were diversely expressed in different organs and tissues, with most of them responding actively to drought- and salt-induced stresses, suggesting the critical roles played by them in various biological processes. Overall, our study shows that GmADF genes may play a crucial role in response to various abiotic stresses in soybean, and the highly inducible candidate genes could be used for further functional studies and molecular breeding in soybean.
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spelling pubmed-104132652023-08-11 Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max) Sun, Yongwang Wang, Deying Shi, Mengmeng Gong, Yujie Yin, Shuwen Jiao, Yexuan Guo, Shangjing Front Plant Sci Plant Science The actin-depolymerizing factor (ADF) encoded by a family of genes is highly conserved among eukaryotes and plays critical roles in the various processes of plant growth, development, and stress responses via the remodeling of the architecture of the actin cytoskeleton. However, the ADF family and the encoded proteins in soybean (Glycine max) have not yet been systematically investigated. In this study, 18 GmADF genes (GmADF1 – GmADF18) were identified in the soybean genome and were mapped to 14 different chromosomes. Phylogenetic analysis classified them into four groups, which was confirmed by their structure and the distribution of conserved motifs in the encoded proteins. Additionally, 29 paralogous gene pairs were identified in the GmADF family, and analysis of their Ka/Ks ratios indicated their purity-based selection during the evolutionary expansion of the soybean genome. The analysis of the expression profiles based on the RNA-seq and qRT-PCR data indicated that GmADFs were diversely expressed in different organs and tissues, with most of them responding actively to drought- and salt-induced stresses, suggesting the critical roles played by them in various biological processes. Overall, our study shows that GmADF genes may play a crucial role in response to various abiotic stresses in soybean, and the highly inducible candidate genes could be used for further functional studies and molecular breeding in soybean. Frontiers Media S.A. 2023-07-27 /pmc/articles/PMC10413265/ /pubmed/37575943 http://dx.doi.org/10.3389/fpls.2023.1236175 Text en Copyright © 2023 Sun, Wang, Shi, Gong, Yin, Jiao and Guo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sun, Yongwang
Wang, Deying
Shi, Mengmeng
Gong, Yujie
Yin, Shuwen
Jiao, Yexuan
Guo, Shangjing
Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title_full Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title_fullStr Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title_full_unstemmed Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title_short Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Glycine max)
title_sort genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (glycine max)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413265/
https://www.ncbi.nlm.nih.gov/pubmed/37575943
http://dx.doi.org/10.3389/fpls.2023.1236175
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