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Tetrandrine Derivatives as Promising Antibacterial Agents
[Image: see text] This work reports on the antibacterial activity of two tetrandrine derivatives, with acridine (MAcT) and anthracene (MAnT) units, against Gram-positive and Gram-negative bacteria of clinical importance by the broth microdilution method as well as their antioxidant activity against...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413380/ https://www.ncbi.nlm.nih.gov/pubmed/37576675 http://dx.doi.org/10.1021/acsomega.3c01368 |
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author | Calvillo-Páez, Viviana I. Plascencia-Jatomea, Maribel Ochoa-Terán, Adrián Del-Toro-Sánchez, Carmen L. González-Vega, Ricardo I. González-Martínez, Sandra M. Ochoa Lara, Karen |
author_facet | Calvillo-Páez, Viviana I. Plascencia-Jatomea, Maribel Ochoa-Terán, Adrián Del-Toro-Sánchez, Carmen L. González-Vega, Ricardo I. González-Martínez, Sandra M. Ochoa Lara, Karen |
author_sort | Calvillo-Páez, Viviana I. |
collection | PubMed |
description | [Image: see text] This work reports on the antibacterial activity of two tetrandrine derivatives, with acridine (MAcT) and anthracene (MAnT) units, against Gram-positive and Gram-negative bacteria of clinical importance by the broth microdilution method as well as their antioxidant activity against ABTS(•+) and DPPH(•+) radicals. Unlike natural tetrandrine, its derivatives inhibited bacterial growth, showing selectivity against Staphylococcus aureus with notable activity of MAnT (MIC = 0.035 μg/mL); this compound also has good activity against the ABTS(•+) radical (IC(50) = 4.59 μg/mL). Cell membrane integrity studies and reactive oxygen species (ROS) detection by fluorescent stains helped to understand possible mechanisms related to antibacterial activity, while electrophoretic mobility assays showed that the derivatives can bind to bacterial DNA plasmid. The results indicate that MAnT can induce a general state of oxidative stress in S. aureus and Escherichia coli, while MAcT induces an oxidative response in S. aureus. Complementary electrochemical studies were included. |
format | Online Article Text |
id | pubmed-10413380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104133802023-08-11 Tetrandrine Derivatives as Promising Antibacterial Agents Calvillo-Páez, Viviana I. Plascencia-Jatomea, Maribel Ochoa-Terán, Adrián Del-Toro-Sánchez, Carmen L. González-Vega, Ricardo I. González-Martínez, Sandra M. Ochoa Lara, Karen ACS Omega [Image: see text] This work reports on the antibacterial activity of two tetrandrine derivatives, with acridine (MAcT) and anthracene (MAnT) units, against Gram-positive and Gram-negative bacteria of clinical importance by the broth microdilution method as well as their antioxidant activity against ABTS(•+) and DPPH(•+) radicals. Unlike natural tetrandrine, its derivatives inhibited bacterial growth, showing selectivity against Staphylococcus aureus with notable activity of MAnT (MIC = 0.035 μg/mL); this compound also has good activity against the ABTS(•+) radical (IC(50) = 4.59 μg/mL). Cell membrane integrity studies and reactive oxygen species (ROS) detection by fluorescent stains helped to understand possible mechanisms related to antibacterial activity, while electrophoretic mobility assays showed that the derivatives can bind to bacterial DNA plasmid. The results indicate that MAnT can induce a general state of oxidative stress in S. aureus and Escherichia coli, while MAcT induces an oxidative response in S. aureus. Complementary electrochemical studies were included. American Chemical Society 2023-07-26 /pmc/articles/PMC10413380/ /pubmed/37576675 http://dx.doi.org/10.1021/acsomega.3c01368 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Calvillo-Páez, Viviana I. Plascencia-Jatomea, Maribel Ochoa-Terán, Adrián Del-Toro-Sánchez, Carmen L. González-Vega, Ricardo I. González-Martínez, Sandra M. Ochoa Lara, Karen Tetrandrine Derivatives as Promising Antibacterial Agents |
title | Tetrandrine Derivatives
as Promising Antibacterial
Agents |
title_full | Tetrandrine Derivatives
as Promising Antibacterial
Agents |
title_fullStr | Tetrandrine Derivatives
as Promising Antibacterial
Agents |
title_full_unstemmed | Tetrandrine Derivatives
as Promising Antibacterial
Agents |
title_short | Tetrandrine Derivatives
as Promising Antibacterial
Agents |
title_sort | tetrandrine derivatives
as promising antibacterial
agents |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413380/ https://www.ncbi.nlm.nih.gov/pubmed/37576675 http://dx.doi.org/10.1021/acsomega.3c01368 |
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