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Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams
BACKGROUND: Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413538/ https://www.ncbi.nlm.nih.gov/pubmed/37559077 http://dx.doi.org/10.1186/s40104-023-00907-3 |
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author | Jurado-Campos, Alejandro Soria-Meneses, Pedro Javier Arenas-Moreira, María Alonso-Moreno, Carlos Rodríguez-Robledo, Virginia Soler, Ana Josefa Garde, José Julián del Rocío Fernández-Santos, María |
author_facet | Jurado-Campos, Alejandro Soria-Meneses, Pedro Javier Arenas-Moreira, María Alonso-Moreno, Carlos Rodríguez-Robledo, Virginia Soler, Ana Josefa Garde, José Julián del Rocío Fernández-Santos, María |
author_sort | Jurado-Campos, Alejandro |
collection | PubMed |
description | BACKGROUND: Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport. RESULTS: With the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 10(6) spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe(2+)/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C(11) BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples. CONCLUSIONS: The storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10413538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104135382023-08-11 Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams Jurado-Campos, Alejandro Soria-Meneses, Pedro Javier Arenas-Moreira, María Alonso-Moreno, Carlos Rodríguez-Robledo, Virginia Soler, Ana Josefa Garde, José Julián del Rocío Fernández-Santos, María J Anim Sci Biotechnol Research BACKGROUND: Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport. RESULTS: With the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 10(6) spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe(2+)/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C(11) BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples. CONCLUSIONS: The storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2023-08-10 /pmc/articles/PMC10413538/ /pubmed/37559077 http://dx.doi.org/10.1186/s40104-023-00907-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Jurado-Campos, Alejandro Soria-Meneses, Pedro Javier Arenas-Moreira, María Alonso-Moreno, Carlos Rodríguez-Robledo, Virginia Soler, Ana Josefa Garde, José Julián del Rocío Fernández-Santos, María Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title | Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title_full | Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title_fullStr | Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title_full_unstemmed | Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title_short | Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
title_sort | minimizing sperm oxidative stress using nanotechnology for breeding programs in rams |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413538/ https://www.ncbi.nlm.nih.gov/pubmed/37559077 http://dx.doi.org/10.1186/s40104-023-00907-3 |
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