Targeted mRNA demethylation in Arabidopsis using plant m6A editor

BACKGROUND: N6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficult to study the effect of specific RNA methylation. RESULTS: In this study, we...

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Autores principales: Fang, Ruiqiu, Chen, Xiaolong, Shen, Jie, Wang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413771/
https://www.ncbi.nlm.nih.gov/pubmed/37559087
http://dx.doi.org/10.1186/s13007-023-01053-7
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author Fang, Ruiqiu
Chen, Xiaolong
Shen, Jie
Wang, Bin
author_facet Fang, Ruiqiu
Chen, Xiaolong
Shen, Jie
Wang, Bin
author_sort Fang, Ruiqiu
collection PubMed
description BACKGROUND: N6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficult to study the effect of specific RNA methylation. RESULTS: In this study, we report the development of Plant m6A Editors (PMEs) using dLwaCas13a (from L. wadei) and human m6A demethylase ALKBH5 catalytic domain. PMEs specifically demethylates m6A of targeted mRNAs (WUS, STM, FT, SPL3 and SPL9) to increase mRNAs stability. In addition, we discovered that a double ribozyme system can significantly improve the efficiency of RNA editing. CONCLUSION: PMEs specifically demethylates m6A of targeted mRNAs to increase mRNAs stability, suggesting that this engineered tool is instrumental for biotechnological applications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-023-01053-7.
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spelling pubmed-104137712023-08-11 Targeted mRNA demethylation in Arabidopsis using plant m6A editor Fang, Ruiqiu Chen, Xiaolong Shen, Jie Wang, Bin Plant Methods Research BACKGROUND: N6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficult to study the effect of specific RNA methylation. RESULTS: In this study, we report the development of Plant m6A Editors (PMEs) using dLwaCas13a (from L. wadei) and human m6A demethylase ALKBH5 catalytic domain. PMEs specifically demethylates m6A of targeted mRNAs (WUS, STM, FT, SPL3 and SPL9) to increase mRNAs stability. In addition, we discovered that a double ribozyme system can significantly improve the efficiency of RNA editing. CONCLUSION: PMEs specifically demethylates m6A of targeted mRNAs to increase mRNAs stability, suggesting that this engineered tool is instrumental for biotechnological applications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-023-01053-7. BioMed Central 2023-08-09 /pmc/articles/PMC10413771/ /pubmed/37559087 http://dx.doi.org/10.1186/s13007-023-01053-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Fang, Ruiqiu
Chen, Xiaolong
Shen, Jie
Wang, Bin
Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title_full Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title_fullStr Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title_full_unstemmed Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title_short Targeted mRNA demethylation in Arabidopsis using plant m6A editor
title_sort targeted mrna demethylation in arabidopsis using plant m6a editor
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413771/
https://www.ncbi.nlm.nih.gov/pubmed/37559087
http://dx.doi.org/10.1186/s13007-023-01053-7
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AT wangbin targetedmrnademethylationinarabidopsisusingplantm6aeditor

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