Cargando…
3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs
The advent of improved fabrication technologies, particularly 3D printing, has enabled the engineering of bone tissue for patient-specific healing and the fabrication of in vitro tissue models for ex vivo testing. However, inks made from natural polymers often fall short in terms of mechanical stren...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10414018/ https://www.ncbi.nlm.nih.gov/pubmed/37577089 http://dx.doi.org/10.1039/d3ra02786d |
_version_ | 1785087254726705152 |
---|---|
author | Cernencu, Alexandra I. Vlasceanu, George M. Serafim, Andrada Pircalabioru, Gratiela Ionita, Mariana |
author_facet | Cernencu, Alexandra I. Vlasceanu, George M. Serafim, Andrada Pircalabioru, Gratiela Ionita, Mariana |
author_sort | Cernencu, Alexandra I. |
collection | PubMed |
description | The advent of improved fabrication technologies, particularly 3D printing, has enabled the engineering of bone tissue for patient-specific healing and the fabrication of in vitro tissue models for ex vivo testing. However, inks made from natural polymers often fall short in terms of mechanical strength, stability, and the induction of osteogenesis. Our research focused on developing novel printable formulations using a gelatin/pectin polymeric matrix that integrate synergistic reinforcement components i.e. graphene oxide (GO) and oxidized nanocellulose fibers (CNF). Using 3D printing technology and the aforementioned biomaterial composite inks, bone-like scaffolds were created. To simulate critical-sized flaws and demonstrate scaffold fidelity, 3D scaffolds were successfully printed using formulations with varied GO concentrations (0.25, 0.5, and 1% wt with respect to polymer content). The addition of GO to hydrogel inks enhanced not only the compressive modulus but also the printability and scaffold fidelity compared to the pure colloid-gelatin/pectin system. Due to its strong potential for 3D bioprinting, the sample containing 0.5% GO is shown to have the greatest perspectives for bone tissue models and tissue engineering applications. |
format | Online Article Text |
id | pubmed-10414018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-104140182023-08-11 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs Cernencu, Alexandra I. Vlasceanu, George M. Serafim, Andrada Pircalabioru, Gratiela Ionita, Mariana RSC Adv Chemistry The advent of improved fabrication technologies, particularly 3D printing, has enabled the engineering of bone tissue for patient-specific healing and the fabrication of in vitro tissue models for ex vivo testing. However, inks made from natural polymers often fall short in terms of mechanical strength, stability, and the induction of osteogenesis. Our research focused on developing novel printable formulations using a gelatin/pectin polymeric matrix that integrate synergistic reinforcement components i.e. graphene oxide (GO) and oxidized nanocellulose fibers (CNF). Using 3D printing technology and the aforementioned biomaterial composite inks, bone-like scaffolds were created. To simulate critical-sized flaws and demonstrate scaffold fidelity, 3D scaffolds were successfully printed using formulations with varied GO concentrations (0.25, 0.5, and 1% wt with respect to polymer content). The addition of GO to hydrogel inks enhanced not only the compressive modulus but also the printability and scaffold fidelity compared to the pure colloid-gelatin/pectin system. Due to its strong potential for 3D bioprinting, the sample containing 0.5% GO is shown to have the greatest perspectives for bone tissue models and tissue engineering applications. The Royal Society of Chemistry 2023-08-10 /pmc/articles/PMC10414018/ /pubmed/37577089 http://dx.doi.org/10.1039/d3ra02786d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Cernencu, Alexandra I. Vlasceanu, George M. Serafim, Andrada Pircalabioru, Gratiela Ionita, Mariana 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title | 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title_full | 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title_fullStr | 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title_full_unstemmed | 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title_short | 3D double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
title_sort | 3d double-reinforced graphene oxide – nanocellulose biomaterial inks for tissue engineered constructs |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10414018/ https://www.ncbi.nlm.nih.gov/pubmed/37577089 http://dx.doi.org/10.1039/d3ra02786d |
work_keys_str_mv | AT cernencualexandrai 3ddoublereinforcedgrapheneoxidenanocellulosebiomaterialinksfortissueengineeredconstructs AT vlasceanugeorgem 3ddoublereinforcedgrapheneoxidenanocellulosebiomaterialinksfortissueengineeredconstructs AT serafimandrada 3ddoublereinforcedgrapheneoxidenanocellulosebiomaterialinksfortissueengineeredconstructs AT pircalabiorugratiela 3ddoublereinforcedgrapheneoxidenanocellulosebiomaterialinksfortissueengineeredconstructs AT ionitamariana 3ddoublereinforcedgrapheneoxidenanocellulosebiomaterialinksfortissueengineeredconstructs |