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Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters

Proteus mirabilis is an opportunistic pathogen associated with a variety of human infections, including urinary tract infections. The prevalence of P. mirabilis in foods of animal origin and in the manure by-products created in animal production is not well documented. Further, the prevalence and pe...

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Autores principales: Chalmers, Gabhan, Anderson, Rebecca E. V., Murray, Roger, Topp, Edward, Boerlin, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10414651/
https://www.ncbi.nlm.nih.gov/pubmed/37561682
http://dx.doi.org/10.1371/journal.pone.0289703
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author Chalmers, Gabhan
Anderson, Rebecca E. V.
Murray, Roger
Topp, Edward
Boerlin, Patrick
author_facet Chalmers, Gabhan
Anderson, Rebecca E. V.
Murray, Roger
Topp, Edward
Boerlin, Patrick
author_sort Chalmers, Gabhan
collection PubMed
description Proteus mirabilis is an opportunistic pathogen associated with a variety of human infections, including urinary tract infections. The prevalence of P. mirabilis in foods of animal origin and in the manure by-products created in animal production is not well documented. Further, the prevalence and persistence of extended-spectrum cephalosporin (ESC) resistant P. mirabilis is largely unknown. In this study, we characterized ESC-resistant P. mirabilis recovered from various stages of dairy manure anaerobic digestion. Isolates were screened by PCR for bla(CTX-M), bla(CMY) and bla(SHV), and antimicrobial susceptibility testing was performed. Fifty-six P. mirabilis carrying CTX-M were sequenced with short and long read sequencing technologies, and the assembled chromosomes and plasmids were compared. ESC-resistant Proteus was found in four of the six manure digesters, an indication that not all digesters were colonized with resistant strains. Both CTX-M-1 and CTX-M-15 plasmids were found in P. mirabilis isolates. Transfer of plasmid DNA by conjugation was also explored, with ESC-resistance plasmids able to transfer to Escherichia coli at high frequency. We concluded that P. mirabilis can harbour and transfer ESC-resistance genes and plasmids, and may be an overlooked reservoir of antimicrobial resistance.
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spelling pubmed-104146512023-08-11 Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters Chalmers, Gabhan Anderson, Rebecca E. V. Murray, Roger Topp, Edward Boerlin, Patrick PLoS One Research Article Proteus mirabilis is an opportunistic pathogen associated with a variety of human infections, including urinary tract infections. The prevalence of P. mirabilis in foods of animal origin and in the manure by-products created in animal production is not well documented. Further, the prevalence and persistence of extended-spectrum cephalosporin (ESC) resistant P. mirabilis is largely unknown. In this study, we characterized ESC-resistant P. mirabilis recovered from various stages of dairy manure anaerobic digestion. Isolates were screened by PCR for bla(CTX-M), bla(CMY) and bla(SHV), and antimicrobial susceptibility testing was performed. Fifty-six P. mirabilis carrying CTX-M were sequenced with short and long read sequencing technologies, and the assembled chromosomes and plasmids were compared. ESC-resistant Proteus was found in four of the six manure digesters, an indication that not all digesters were colonized with resistant strains. Both CTX-M-1 and CTX-M-15 plasmids were found in P. mirabilis isolates. Transfer of plasmid DNA by conjugation was also explored, with ESC-resistance plasmids able to transfer to Escherichia coli at high frequency. We concluded that P. mirabilis can harbour and transfer ESC-resistance genes and plasmids, and may be an overlooked reservoir of antimicrobial resistance. Public Library of Science 2023-08-10 /pmc/articles/PMC10414651/ /pubmed/37561682 http://dx.doi.org/10.1371/journal.pone.0289703 Text en © 2023 Chalmers et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chalmers, Gabhan
Anderson, Rebecca E. V.
Murray, Roger
Topp, Edward
Boerlin, Patrick
Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title_full Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title_fullStr Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title_full_unstemmed Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title_short Characterization of Proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
title_sort characterization of proteus mirabilis and associated plasmids isolated from anaerobic dairy cattle manure digesters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10414651/
https://www.ncbi.nlm.nih.gov/pubmed/37561682
http://dx.doi.org/10.1371/journal.pone.0289703
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