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circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis
BACKGROUND: Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. METHODS: The detect...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415084/ https://www.ncbi.nlm.nih.gov/pubmed/37575469 http://dx.doi.org/10.1155/2023/1680634 |
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author | Yan, Manli Hu, Caihong Hu, Qi Ma, Heran Lei, Changjiang Liu, Yamei |
author_facet | Yan, Manli Hu, Caihong Hu, Qi Ma, Heran Lei, Changjiang Liu, Yamei |
author_sort | Yan, Manli |
collection | PubMed |
description | BACKGROUND: Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. METHODS: The detection of circ_0008285 level in glioma specimens and cell lines was conducted by quantitative real-time polymerase chain reaction. The chi-squared test was employed to evaluate the relationship between the circ_0008285 level and the clinical features of glioma patients. The roles of circ_0008285 on the proliferation and apoptosis of glioma cells were studied by knockdown experiment. Meanwhile, the regulatory relationship of circ_0008285, miR-384, and high mobility group protein B1 (HMGB1) was explored in glioma cells, and we explored the effects of circ_0008285/miR-384/HMGB1 pathway on glioma cells. RESULTS: In glioma specimens and cell lines, the expression of circ_0008285 was significantly increased, and a high circ_0008285 level was associated with a larger tumor size and more advanced grading in glioma patients. Furthermore, downregulating circ_0008285 suppressed proliferation and triggered apoptosis of glioma cells, which was associated with a cell cycle arrest at the G1/G0 phase. Mechanism studies indicated that circ_0008285 regulated HMGB1 by sponging miR-384. Functional experiments demonstrated that circ_0008285 promoted the malignant phenotype of glioma cells by miR-384/HMGB1 axis. CONCLUSION: Our study revealed circ_0008285 as a novel oncogenic factor in glioma through modulating the miR-384/HMGB1 pathway, suggesting that targeting circ_0008285 could serve as a strategy for glioma management. |
format | Online Article Text |
id | pubmed-10415084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-104150842023-08-11 circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis Yan, Manli Hu, Caihong Hu, Qi Ma, Heran Lei, Changjiang Liu, Yamei Int J Genomics Research Article BACKGROUND: Recent studies indicate that circular RNAs (circRNAs) have been implicated in the initiation or progression of a wide spectrum of diseases. In the current study, we explored the potential engagement of circ_0008285 in glioma and investigated the downstream regulators. METHODS: The detection of circ_0008285 level in glioma specimens and cell lines was conducted by quantitative real-time polymerase chain reaction. The chi-squared test was employed to evaluate the relationship between the circ_0008285 level and the clinical features of glioma patients. The roles of circ_0008285 on the proliferation and apoptosis of glioma cells were studied by knockdown experiment. Meanwhile, the regulatory relationship of circ_0008285, miR-384, and high mobility group protein B1 (HMGB1) was explored in glioma cells, and we explored the effects of circ_0008285/miR-384/HMGB1 pathway on glioma cells. RESULTS: In glioma specimens and cell lines, the expression of circ_0008285 was significantly increased, and a high circ_0008285 level was associated with a larger tumor size and more advanced grading in glioma patients. Furthermore, downregulating circ_0008285 suppressed proliferation and triggered apoptosis of glioma cells, which was associated with a cell cycle arrest at the G1/G0 phase. Mechanism studies indicated that circ_0008285 regulated HMGB1 by sponging miR-384. Functional experiments demonstrated that circ_0008285 promoted the malignant phenotype of glioma cells by miR-384/HMGB1 axis. CONCLUSION: Our study revealed circ_0008285 as a novel oncogenic factor in glioma through modulating the miR-384/HMGB1 pathway, suggesting that targeting circ_0008285 could serve as a strategy for glioma management. Hindawi 2023-08-03 /pmc/articles/PMC10415084/ /pubmed/37575469 http://dx.doi.org/10.1155/2023/1680634 Text en Copyright © 2023 Manli Yan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yan, Manli Hu, Caihong Hu, Qi Ma, Heran Lei, Changjiang Liu, Yamei circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title | circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title_full | circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title_fullStr | circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title_full_unstemmed | circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title_short | circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis |
title_sort | circ_0008285 regulates glioma progression via the mir-384/hmgb1 axis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415084/ https://www.ncbi.nlm.nih.gov/pubmed/37575469 http://dx.doi.org/10.1155/2023/1680634 |
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