Cargando…

Highly active CRISPR-adaptation proteins revealed by a robust enrichment technology

Natural prokaryotic defense via the CRISPR–Cas system requires spacer integration into the CRISPR array in a process called adaptation. To search for adaptation proteins with enhanced capabilities, we established a robust perpetual DNA packaging and transfer (PeDPaT) system that uses a strain of T7...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yosef, Ido, Mahata, Tridib, Goren, Moran G, Degany, Or J, Ben-Shem, Adam, Qimron, Udi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415146/ https://www.ncbi.nlm.nih.gov/pubmed/37326009 http://dx.doi.org/10.1093/nar/gkad510

Ejemplares similares

An efficient, scarless, selection-free technology for phage engineering
por: Goren, Moran G., et al.
Publicado: (2023)

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli
por: Yosef, Ido, et al.
Publicado: (2012)

Caught in the act: the lifetime of synaptic intermediates during the search for homology on DNA
por: Mani, Adam, et al.
Publicado: (2010)

Cas1–Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation
por: Killelea, Tom, et al.
Publicado: (2023)

Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation
por: Kuznedelov, Konstantin, et al.
Publicado: (2016)

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system
por: Sokolowski, Richard D., et al.
Publicado: (2014)

The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence
por: Zhu, Wenlong, et al.
Publicado: (2021)

Structure and mechanism of the type I-G CRISPR effector
por: Shangguan, Qilin, et al.
Publicado: (2022)

Novel molecular requirements for CRISPR RNA-guided transposition
por: Walker, Matt W G, et al.
Publicado: (2023)

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
por: Kato-Inui, Tomoko, et al.
Publicado: (2018)

Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
por: Okada, Keita, et al.
Publicado: (2022)

Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence
por: Grüschow, Sabine, et al.
Publicado: (2019)

Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA
por: Paul, Bijoya, et al.
Publicado: (2021)

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems
por: Zhang, Jing, et al.
Publicado: (2016)

Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
por: Zhang, Liyang, et al.
Publicado: (2020)

Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system
por: Gleditzsch, Daniel, et al.
Publicado: (2016)

DNA targeting by Clostridium cellulolyticum CRISPR–Cas9 Type II-C system
por: Fedorova, Iana, et al.
Publicado: (2020)

CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
por: Marino, Nicole D, et al.
Publicado: (2022)

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex
por: Plagens, André, et al.
Publicado: (2014)

Interference activity of a minimal Type I CRISPR–Cas system from Shewanella putrefaciens
por: Dwarakanath, Srivatsa, et al.
Publicado: (2015)

Different modes of spacer acquisition by the Staphylococcus epidermidis type III-A CRISPR-Cas system
por: Aviram, Naama, et al.
Publicado: (2022)

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing
por: Song, Guoxu, et al.
Publicado: (2022)

Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases
por: Molina, Rafael, et al.
Publicado: (2022)

A highly specific sodium aptamer probed by 2-aminopurine for robust Na(+) sensing
por: Zhou, Wenhu, et al.
Publicado: (2016)

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
por: Mekler, Vladimir, et al.
Publicado: (2016)

Casposon integration shows strong target site preference and recapitulates protospacer integration by CRISPR-Cas systems
por: Béguin, Pierre, et al.
Publicado: (2016)

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction
por: Han, Wenyuan, et al.
Publicado: (2017)

Fuse to defuse: a self-limiting ribonuclease-ring nuclease fusion for type III CRISPR defence
por: Samolygo, Aleksei, et al.
Publicado: (2020)

Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide
por: Kim, Hanseop, et al.
Publicado: (2020)

Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
por: Murugan, Karthik, et al.
Publicado: (2021)

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
por: Tao, Rui, et al.
Publicado: (2022)

A DNA unwinding equilibrium serves as a checkpoint for CRISPR-Cas12a target discrimination
por: Singh, Jaideep, et al.
Publicado: (2023)

DNA binding specificities of Escherichia coli Cas1–Cas2 integrase drive its recruitment at the CRISPR locus
por: Moch, Clara, et al.
Publicado: (2017)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes
por: Vlot, Marnix, et al.
Publicado: (2018)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems
por: Fonfara, Ines, et al.
Publicado: (2014)

Active site plasticity enables metal-dependent tuning of Cas5d nuclease activity in CRISPR-Cas type I-C system
por: Punetha, Ankita, et al.
Publicado: (2014)

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA
por: Li, Yingjun, et al.
Publicado: (2017)

A type III-A CRISPR–Cas system mediates co-transcriptional DNA cleavage at the transcriptional bubbles in close proximity to active effectors
por: Lin, Jinzhong, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_6a676r7a3vm8l8cfbhs0lje9pg