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Highly active CRISPR-adaptation proteins revealed by a robust enrichment technology

Natural prokaryotic defense via the CRISPR–Cas system requires spacer integration into the CRISPR array in a process called adaptation. To search for adaptation proteins with enhanced capabilities, we established a robust perpetual DNA packaging and transfer (PeDPaT) system that uses a strain of T7...

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Detalles Bibliográficos
Autores principales:	Yosef, Ido, Mahata, Tridib, Goren, Moran G, Degany, Or J, Ben-Shem, Adam, Qimron, Udi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415146/ https://www.ncbi.nlm.nih.gov/pubmed/37326009 http://dx.doi.org/10.1093/nar/gkad510

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