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A Simple Sonication Method to Isolate the Chloroplast Lumen in Arabidopsis thaliana
The chloroplast lumen contains at least 80 proteins whose function and regulation are not yet fully understood. Isolating the chloroplast lumen enables the characterization of the lumenal proteins. The lumen can be isolated in several ways through thylakoid disruption using a Yeda press or sonicatio...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415170/ https://www.ncbi.nlm.nih.gov/pubmed/37575389 http://dx.doi.org/10.21769/BioProtoc.4756 |