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A Simple Sonication Method to Isolate the Chloroplast Lumen in Arabidopsis thaliana

The chloroplast lumen contains at least 80 proteins whose function and regulation are not yet fully understood. Isolating the chloroplast lumen enables the characterization of the lumenal proteins. The lumen can be isolated in several ways through thylakoid disruption using a Yeda press or sonicatio...

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Detalles Bibliográficos
Autores principales: Hao, Jingfang, Malnoë, Alizée
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415170/
https://www.ncbi.nlm.nih.gov/pubmed/37575389
http://dx.doi.org/10.21769/BioProtoc.4756