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A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes

Study of gene function in eukaryotes frequently requires data on the impact of the gene when it is expressed as a transgene, such as in ectopic or overexpression studies. Currently, the use of transgenic constructs designed to achieve these aims is often hampered by the difficulty in distinguishing...

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Detalles Bibliográficos
Autores principales: Bezodis, William, Prescott, Helen, Vlad, Daniela, Dickinson, Hugh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415200/
https://www.ncbi.nlm.nih.gov/pubmed/37575385
http://dx.doi.org/10.21769/BioProtoc.4784

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