Cargando…

Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia

BACKGROUND: Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections and the most common antimicrobial-resistant pathogens. It is associated with a variety of infections. This study aimed to determine the prevalence of P. aeruginosa and its antimicrobial resistance profile...

Descripción completa

Detalles Bibliográficos
Autores principales: Asamenew, Tsigereda, Worku, Seble, Motbainor, Hilina, Mekonnen, Daniel, Deribe, Awoke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Publications Office of Jimma University 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416326/
https://www.ncbi.nlm.nih.gov/pubmed/37576170
http://dx.doi.org/10.4314/ejhs.v33i3.5
_version_ 1785087747163160576
author Asamenew, Tsigereda
Worku, Seble
Motbainor, Hilina
Mekonnen, Daniel
Deribe, Awoke
author_facet Asamenew, Tsigereda
Worku, Seble
Motbainor, Hilina
Mekonnen, Daniel
Deribe, Awoke
author_sort Asamenew, Tsigereda
collection PubMed
description BACKGROUND: Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections and the most common antimicrobial-resistant pathogens. It is associated with a variety of infections. This study aimed to determine the prevalence of P. aeruginosa and its antimicrobial resistance profile from different clinical specimens at Debre Tabor Comprehensive Referral Hospital (DTCRH). METHODS: A cross-sectional study was conducted from May to July 2022 at DTCRH. Socio-demographic and clinical data were collected using a structured questionnaire. Clinical samples (blood, wound swab, urine, and sputum) were collected from 348 study participants and processed following the standard bacteriological techniques. Antibiotic susceptibility testing was done by the Kirby-Bauer disc diffusion method. Data were entered and analyzed using SPSS version 25 statistical software. Descriptive statistics was used to present the findings of the study. RESULTS: The prevalence of P.aeruginosa was 74(19.3%). The detection of the isolates was different based on the type of samples that ranged from 0% to 54.5% from sputum and wound swabs, respectively. P.aeruginosa showed resistance against gentamicin at 62.2%, ceftazidime 51.4%, cefepime 50%, amikacin 29.7%, imipenem 28.4% and ciprofloxacin 14.9%. The level of multi-drug resistance (MDR) was 45.9%, and the suspicious extreme-drug resistance (XDR) rate was 9.5%. Being inpatient and wound swab samples were factors associated with the detection of P.aeruginosa from clinical samples. CONCLUSION: The antibiotic resistance profile of P. aeruginosa isolates in the present study area was found to be alarming. Actions to minimize the effect of antimicrobial resistance should be strengthened, and further large-scale study should be conducted to find out the main reasons behind antibiotic resistance of P.aeruginosa and other clinically relevant isolates.
format Online
Article
Text
id pubmed-10416326
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Research and Publications Office of Jimma University
record_format MEDLINE/PubMed
spelling pubmed-104163262023-08-12 Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia Asamenew, Tsigereda Worku, Seble Motbainor, Hilina Mekonnen, Daniel Deribe, Awoke Ethiop J Health Sci Original Article BACKGROUND: Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections and the most common antimicrobial-resistant pathogens. It is associated with a variety of infections. This study aimed to determine the prevalence of P. aeruginosa and its antimicrobial resistance profile from different clinical specimens at Debre Tabor Comprehensive Referral Hospital (DTCRH). METHODS: A cross-sectional study was conducted from May to July 2022 at DTCRH. Socio-demographic and clinical data were collected using a structured questionnaire. Clinical samples (blood, wound swab, urine, and sputum) were collected from 348 study participants and processed following the standard bacteriological techniques. Antibiotic susceptibility testing was done by the Kirby-Bauer disc diffusion method. Data were entered and analyzed using SPSS version 25 statistical software. Descriptive statistics was used to present the findings of the study. RESULTS: The prevalence of P.aeruginosa was 74(19.3%). The detection of the isolates was different based on the type of samples that ranged from 0% to 54.5% from sputum and wound swabs, respectively. P.aeruginosa showed resistance against gentamicin at 62.2%, ceftazidime 51.4%, cefepime 50%, amikacin 29.7%, imipenem 28.4% and ciprofloxacin 14.9%. The level of multi-drug resistance (MDR) was 45.9%, and the suspicious extreme-drug resistance (XDR) rate was 9.5%. Being inpatient and wound swab samples were factors associated with the detection of P.aeruginosa from clinical samples. CONCLUSION: The antibiotic resistance profile of P. aeruginosa isolates in the present study area was found to be alarming. Actions to minimize the effect of antimicrobial resistance should be strengthened, and further large-scale study should be conducted to find out the main reasons behind antibiotic resistance of P.aeruginosa and other clinically relevant isolates. Research and Publications Office of Jimma University 2023-05 /pmc/articles/PMC10416326/ /pubmed/37576170 http://dx.doi.org/10.4314/ejhs.v33i3.5 Text en © 2023 Tsigereda Asminew, et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Asamenew, Tsigereda
Worku, Seble
Motbainor, Hilina
Mekonnen, Daniel
Deribe, Awoke
Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title_full Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title_fullStr Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title_full_unstemmed Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title_short Antimicrobial Resistance Profile of Pseudomonas aeruginosa from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia
title_sort antimicrobial resistance profile of pseudomonas aeruginosa from different clinical samples in debre tabor comprehensive specialized hospital, northwest ethiopia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416326/
https://www.ncbi.nlm.nih.gov/pubmed/37576170
http://dx.doi.org/10.4314/ejhs.v33i3.5
work_keys_str_mv AT asamenewtsigereda antimicrobialresistanceprofileofpseudomonasaeruginosafromdifferentclinicalsamplesindebretaborcomprehensivespecializedhospitalnorthwestethiopia
AT workuseble antimicrobialresistanceprofileofpseudomonasaeruginosafromdifferentclinicalsamplesindebretaborcomprehensivespecializedhospitalnorthwestethiopia
AT motbainorhilina antimicrobialresistanceprofileofpseudomonasaeruginosafromdifferentclinicalsamplesindebretaborcomprehensivespecializedhospitalnorthwestethiopia
AT mekonnendaniel antimicrobialresistanceprofileofpseudomonasaeruginosafromdifferentclinicalsamplesindebretaborcomprehensivespecializedhospitalnorthwestethiopia
AT deribeawoke antimicrobialresistanceprofileofpseudomonasaeruginosafromdifferentclinicalsamplesindebretaborcomprehensivespecializedhospitalnorthwestethiopia