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Recent advances in in situ Notch signaling measurement

Notch signaling is necessary for the development of many organ systems, including the nervous system, biliary system, and visual and auditory sensory systems. This signaling pathway is composed of DSL ligands and Notch receptors. Upon the interaction of those components between neighboring cells, th...

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Autores principales: Yoshihara, Masaharu, Takahashi, Satoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416437/
https://www.ncbi.nlm.nih.gov/pubmed/37576594
http://dx.doi.org/10.3389/fcell.2023.1244105
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author Yoshihara, Masaharu
Takahashi, Satoru
author_facet Yoshihara, Masaharu
Takahashi, Satoru
author_sort Yoshihara, Masaharu
collection PubMed
description Notch signaling is necessary for the development of many organ systems, including the nervous system, biliary system, and visual and auditory sensory systems. This signaling pathway is composed of DSL ligands and Notch receptors. Upon the interaction of those components between neighboring cells, the intracellular domain of the Notch receptor is cleaved from the cell membrane to act as a transcription factor. To date, many mechanistic insights, including lateral inhibition and lateral induction, have been proposed from observation of patterning morphogenesis and expression profiles of Notch signaling-associated molecules. The lack of a direct measurement method for Notch signaling, however, has impeded the examination of those mechanistic insights. In this mini-review, recent advances in the direct measurement of Notch signaling are introduced with a focus on the application of genetic modification of Notch receptors with the components of the Cre/loxP system and Gal4/UAS system. The combination of such conventional genetic techniques is opening a new era in Notch signaling biology by direct visualization of Notch “signaling” in addition to Notch signaling-associated molecules.
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spelling pubmed-104164372023-08-12 Recent advances in in situ Notch signaling measurement Yoshihara, Masaharu Takahashi, Satoru Front Cell Dev Biol Cell and Developmental Biology Notch signaling is necessary for the development of many organ systems, including the nervous system, biliary system, and visual and auditory sensory systems. This signaling pathway is composed of DSL ligands and Notch receptors. Upon the interaction of those components between neighboring cells, the intracellular domain of the Notch receptor is cleaved from the cell membrane to act as a transcription factor. To date, many mechanistic insights, including lateral inhibition and lateral induction, have been proposed from observation of patterning morphogenesis and expression profiles of Notch signaling-associated molecules. The lack of a direct measurement method for Notch signaling, however, has impeded the examination of those mechanistic insights. In this mini-review, recent advances in the direct measurement of Notch signaling are introduced with a focus on the application of genetic modification of Notch receptors with the components of the Cre/loxP system and Gal4/UAS system. The combination of such conventional genetic techniques is opening a new era in Notch signaling biology by direct visualization of Notch “signaling” in addition to Notch signaling-associated molecules. Frontiers Media S.A. 2023-07-28 /pmc/articles/PMC10416437/ /pubmed/37576594 http://dx.doi.org/10.3389/fcell.2023.1244105 Text en Copyright © 2023 Yoshihara and Takahashi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Yoshihara, Masaharu
Takahashi, Satoru
Recent advances in in situ Notch signaling measurement
title Recent advances in in situ Notch signaling measurement
title_full Recent advances in in situ Notch signaling measurement
title_fullStr Recent advances in in situ Notch signaling measurement
title_full_unstemmed Recent advances in in situ Notch signaling measurement
title_short Recent advances in in situ Notch signaling measurement
title_sort recent advances in in situ notch signaling measurement
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416437/
https://www.ncbi.nlm.nih.gov/pubmed/37576594
http://dx.doi.org/10.3389/fcell.2023.1244105
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