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Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation

BACKGROUND: Cardiomyocyte growth and differentiation rely on precise gene expression regulation, with epigenetic modifications emerging as key players in this intricate process. Among these modifications, N6-methyladenosine (m6A) stands out as one of the most prevalent modifications on mRNA, exertin...

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Autores principales: Liu, Xue-Hong, Liu, Zhun, Ren, Ze-Hui, Chen, Hong-Xuan, Zhang, Ying, Zhang, Zhang, Cao, Nan, Luo, Guan-Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416456/
https://www.ncbi.nlm.nih.gov/pubmed/37568210
http://dx.doi.org/10.1186/s13072-023-00506-6
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author Liu, Xue-Hong
Liu, Zhun
Ren, Ze-Hui
Chen, Hong-Xuan
Zhang, Ying
Zhang, Zhang
Cao, Nan
Luo, Guan-Zheng
author_facet Liu, Xue-Hong
Liu, Zhun
Ren, Ze-Hui
Chen, Hong-Xuan
Zhang, Ying
Zhang, Zhang
Cao, Nan
Luo, Guan-Zheng
author_sort Liu, Xue-Hong
collection PubMed
description BACKGROUND: Cardiomyocyte growth and differentiation rely on precise gene expression regulation, with epigenetic modifications emerging as key players in this intricate process. Among these modifications, N6-methyladenosine (m6A) stands out as one of the most prevalent modifications on mRNA, exerting influence over mRNA metabolism and gene expression. However, the specific function of m6A in cardiomyocyte differentiation remains poorly understood. RESULTS: We investigated the relationship between m6A modification and cardiomyocyte differentiation by conducting a comprehensive profiling of m6A dynamics during the transition from pluripotent stem cells to cardiomyocytes. Our findings reveal that while the overall m6A modification level remains relatively stable, the m6A levels of individual genes undergo significant changes throughout cardiomyocyte differentiation. We discovered the correlation between alterations in chromatin accessibility and the binding capabilities of m6A writers, erasers, and readers. The changes in chromatin accessibility influence the recruitment and activity of m6A regulatory proteins, thereby impacting the levels of m6A modification on specific mRNA transcripts. CONCLUSION: Our data demonstrate that the coordinated dynamics of m6A modification and chromatin accessibility are prominent during the cardiomyocyte differentiation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-023-00506-6.
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spelling pubmed-104164562023-08-12 Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation Liu, Xue-Hong Liu, Zhun Ren, Ze-Hui Chen, Hong-Xuan Zhang, Ying Zhang, Zhang Cao, Nan Luo, Guan-Zheng Epigenetics Chromatin Research BACKGROUND: Cardiomyocyte growth and differentiation rely on precise gene expression regulation, with epigenetic modifications emerging as key players in this intricate process. Among these modifications, N6-methyladenosine (m6A) stands out as one of the most prevalent modifications on mRNA, exerting influence over mRNA metabolism and gene expression. However, the specific function of m6A in cardiomyocyte differentiation remains poorly understood. RESULTS: We investigated the relationship between m6A modification and cardiomyocyte differentiation by conducting a comprehensive profiling of m6A dynamics during the transition from pluripotent stem cells to cardiomyocytes. Our findings reveal that while the overall m6A modification level remains relatively stable, the m6A levels of individual genes undergo significant changes throughout cardiomyocyte differentiation. We discovered the correlation between alterations in chromatin accessibility and the binding capabilities of m6A writers, erasers, and readers. The changes in chromatin accessibility influence the recruitment and activity of m6A regulatory proteins, thereby impacting the levels of m6A modification on specific mRNA transcripts. CONCLUSION: Our data demonstrate that the coordinated dynamics of m6A modification and chromatin accessibility are prominent during the cardiomyocyte differentiation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-023-00506-6. BioMed Central 2023-08-11 /pmc/articles/PMC10416456/ /pubmed/37568210 http://dx.doi.org/10.1186/s13072-023-00506-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Xue-Hong
Liu, Zhun
Ren, Ze-Hui
Chen, Hong-Xuan
Zhang, Ying
Zhang, Zhang
Cao, Nan
Luo, Guan-Zheng
Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title_full Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title_fullStr Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title_full_unstemmed Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title_short Co-effects of m6A and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
title_sort co-effects of m6a and chromatin accessibility dynamics in the regulation of cardiomyocyte differentiation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416456/
https://www.ncbi.nlm.nih.gov/pubmed/37568210
http://dx.doi.org/10.1186/s13072-023-00506-6
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