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Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage

Recurrent miscarriage (RM) is a pregnancy complication associated with dysregulation of the maternal-fetal interface. We aimed to identify dysfunctional interactions between trophoblast cells and decidual immune cells in RM. We downloaded single-cell RNA sequencing (scRNA-seq) datasets (GSE214607) f...

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Autores principales: Liu, Zhenzhen, Tang, Yao, Zhang, Xiaoyue, Pei, Jiangnan, Wang, Chengjie, Liu, Haiyan, Yu, Yi, Luo, Shouling, Gu, Weirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416716/
https://www.ncbi.nlm.nih.gov/pubmed/37575278
http://dx.doi.org/10.7150/ijms.86533
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author Liu, Zhenzhen
Tang, Yao
Zhang, Xiaoyue
Pei, Jiangnan
Wang, Chengjie
Liu, Haiyan
Yu, Yi
Luo, Shouling
Gu, Weirong
author_facet Liu, Zhenzhen
Tang, Yao
Zhang, Xiaoyue
Pei, Jiangnan
Wang, Chengjie
Liu, Haiyan
Yu, Yi
Luo, Shouling
Gu, Weirong
author_sort Liu, Zhenzhen
collection PubMed
description Recurrent miscarriage (RM) is a pregnancy complication associated with dysregulation of the maternal-fetal interface. We aimed to identify dysfunctional interactions between trophoblast cells and decidual immune cells in RM. We downloaded single-cell RNA sequencing (scRNA-seq) datasets (GSE214607) from the Gene Expression Omnibus (GEO) datasets for further analysis using the R software. The data comprised of paired placental and decidual tissues, including those from patients diagnosed with RM and matched healthy controls. A total of 22976 cells were identified in 11 cell types, including trophoblasts, immune cells, and other cells. We divided trophoblast cells into three types and analyzed their interactions with decidual immune cells. Additionally, we re-clustered NK&T cells and macrophages, identified differentially expressed genes (DEGs), enriched their functions, and compared the cell interactions with trophoblast cells in each cell type. Our single-cell atlas of the maternal-fetal interface revealed alterations in the cellular organization of the decidua and placenta, cell type-specific transcriptome, and cell communication between immune and non-immune cells in RM, which are critical for illuminating the pathophysiology of RM.
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spelling pubmed-104167162023-08-12 Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage Liu, Zhenzhen Tang, Yao Zhang, Xiaoyue Pei, Jiangnan Wang, Chengjie Liu, Haiyan Yu, Yi Luo, Shouling Gu, Weirong Int J Med Sci Research Paper Recurrent miscarriage (RM) is a pregnancy complication associated with dysregulation of the maternal-fetal interface. We aimed to identify dysfunctional interactions between trophoblast cells and decidual immune cells in RM. We downloaded single-cell RNA sequencing (scRNA-seq) datasets (GSE214607) from the Gene Expression Omnibus (GEO) datasets for further analysis using the R software. The data comprised of paired placental and decidual tissues, including those from patients diagnosed with RM and matched healthy controls. A total of 22976 cells were identified in 11 cell types, including trophoblasts, immune cells, and other cells. We divided trophoblast cells into three types and analyzed their interactions with decidual immune cells. Additionally, we re-clustered NK&T cells and macrophages, identified differentially expressed genes (DEGs), enriched their functions, and compared the cell interactions with trophoblast cells in each cell type. Our single-cell atlas of the maternal-fetal interface revealed alterations in the cellular organization of the decidua and placenta, cell type-specific transcriptome, and cell communication between immune and non-immune cells in RM, which are critical for illuminating the pathophysiology of RM. Ivyspring International Publisher 2023-07-31 /pmc/articles/PMC10416716/ /pubmed/37575278 http://dx.doi.org/10.7150/ijms.86533 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Liu, Zhenzhen
Tang, Yao
Zhang, Xiaoyue
Pei, Jiangnan
Wang, Chengjie
Liu, Haiyan
Yu, Yi
Luo, Shouling
Gu, Weirong
Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title_full Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title_fullStr Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title_full_unstemmed Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title_short Crosstalk between Placental Trophoblast and Decidual Immune Cells in Recurrent Miscarriage
title_sort crosstalk between placental trophoblast and decidual immune cells in recurrent miscarriage
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416716/
https://www.ncbi.nlm.nih.gov/pubmed/37575278
http://dx.doi.org/10.7150/ijms.86533
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