Association between Polymorphism in the Janus Kinase 2 (JAK2) Gene and Selected Performance Traits in Cattle and Sheep

SIMPLE SUMMARY: An important element of livestock breeding is the selection that leads to the consolidation and improvement of the traits with utility value. The use of single-nucleotide polymorphism (SNP) markers offers the opportunity to select the appropriate genotype linked to the phenotype, which manifests itself in significant breeding benefits. Polymorphic sites within the genes coding the somatotropic axis are directly and indirectly associated with the phenotype, particularly concerning the production properties of growth and carcass as well as reproductive function. This study analyzed the effect of selected polymorphisms in the Janus Kinase 2 (JAK2) gene on performance characteristics of cattle and sheep. The impact of the mutations on the development of selected traits, which are important from the economic standpoint, was evaluated. Such studies can be utilized to enhance the growth characteristics of cattle and sheep, thereby facilitating the development of more profitable and sustainable breeding methods. ABSTRACT: The Janus Kinase 2 (JAK2) tyrosine kinase is an essential component of signal transduction of the class II cytokine receptors, including the growth hormone receptor. Therefore, it may play a crucial role in the signaling pathway of the somatotropic axis, which influences growth, development, and reproductive traits in ruminants. For this purpose, for three breeds of cattle (Hereford, Angus, and Limousin; a total of 781 individuals), two polymorphic sites located in exon 16 (rs210148032; p.Ile704Val, within pseudokinase (JH2)) and exon 23 (silent mutation rs211067160, within JH1 kinase domain) were analyzed. For two breeds of sheep (Pomeranian and Suffolk; 333 individuals in total), two polymorphic sites in exon 6 (rs160146162 and rs160146160; encoding the FERM domain) and one polymorphic site in exon 24 of the JAK2 gene (rs160146116; JH1 kinase domain) were genotyped. In our study, the associations examined for cattle were inconclusive. However, Hereford and Limousin cattle with genotypes AA (e16/RsaI) and AA (e23/HaeIII) tended to have the highest body weight and better daily gains (p ≤ 0.05). No clear tendency was observed in the selected reproductive traits. In the case of sheep, regardless of breed, individuals with the AA (e6/EarI), GG (e6/seq), and AA (e24/Hpy188III) genotypes had the highest body weights and daily gains in the study periods (p ≤ 0.01). The same individuals in the Pomeranian breed also had better fertility and lamb survival (p ≤ 0.01). To the best of our knowledge, these are the first association studies for all these polymorphic sites. Single-nucleotide polymorphisms in the JAK2 gene can serve as genetic markers for growth and selected reproductive traits in ruminants given that they are further investigated in subsequent populations and analyzed using haplotype and/or combined genotype systems.