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Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility

In contracting muscles, carbohydrates and fatty acids serve as energy substrates; the predominant utilization depends on the workload. Here, we investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in response to repeated training in a polygenic, paternally selected...

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Autores principales: Brenmoehl, Julia, Brosig, Elli, Trakooljul, Nares, Walz, Christina, Ohde, Daniela, Noce, Antonia, Walz, Michael, Langhammer, Martina, Petkov, Stefan, Röntgen, Monika, Maak, Steffen, Galuska, Christina E., Fuchs, Beate, Kuhla, Björn, Ponsuksili, Siriluck, Wimmers, Klaus, Hoeflich, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417281/
https://www.ncbi.nlm.nih.gov/pubmed/37566003
http://dx.doi.org/10.3390/cells12151925
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author Brenmoehl, Julia
Brosig, Elli
Trakooljul, Nares
Walz, Christina
Ohde, Daniela
Noce, Antonia
Walz, Michael
Langhammer, Martina
Petkov, Stefan
Röntgen, Monika
Maak, Steffen
Galuska, Christina E.
Fuchs, Beate
Kuhla, Björn
Ponsuksili, Siriluck
Wimmers, Klaus
Hoeflich, Andreas
author_facet Brenmoehl, Julia
Brosig, Elli
Trakooljul, Nares
Walz, Christina
Ohde, Daniela
Noce, Antonia
Walz, Michael
Langhammer, Martina
Petkov, Stefan
Röntgen, Monika
Maak, Steffen
Galuska, Christina E.
Fuchs, Beate
Kuhla, Björn
Ponsuksili, Siriluck
Wimmers, Klaus
Hoeflich, Andreas
author_sort Brenmoehl, Julia
collection PubMed
description In contracting muscles, carbohydrates and fatty acids serve as energy substrates; the predominant utilization depends on the workload. Here, we investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in response to repeated training in a polygenic, paternally selected marathon mouse model (DUhTP), characterized by exceptional running performance and an unselected control (DUC), with both lines descended from the same genetic background. Both lines underwent three weeks of high-speed treadmill training or were sedentary. Both lines’ muscles and plasma were analyzed. Muscle RNA was sequenced, and KEGG pathway analysis was performed. Analyses of muscle revealed no significant selection-related differences in muscle structure. However, in response to physical exercise, glucose and fatty acid oxidation were stimulated, lactate dehydrogenase activity was reduced, and lactate formation was inhibited in the marathon mice compared with trained control mice. The lack of lactate formation in response to exercise appears to be associated with increased lipid mobilization from peripheral adipose tissue in DUhTP mice, suggesting a specific benefit of lactate avoidance. Thus, results from the analysis of muscle metabolism in born marathon mice, shaped by 35 years (140 generations) of phenotype selection for superior running performance, suggest increased metabolic flexibility in male marathon mice toward lipid catabolism regulated by lactate dehydrogenase.
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spelling pubmed-104172812023-08-12 Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility Brenmoehl, Julia Brosig, Elli Trakooljul, Nares Walz, Christina Ohde, Daniela Noce, Antonia Walz, Michael Langhammer, Martina Petkov, Stefan Röntgen, Monika Maak, Steffen Galuska, Christina E. Fuchs, Beate Kuhla, Björn Ponsuksili, Siriluck Wimmers, Klaus Hoeflich, Andreas Cells Article In contracting muscles, carbohydrates and fatty acids serve as energy substrates; the predominant utilization depends on the workload. Here, we investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in response to repeated training in a polygenic, paternally selected marathon mouse model (DUhTP), characterized by exceptional running performance and an unselected control (DUC), with both lines descended from the same genetic background. Both lines underwent three weeks of high-speed treadmill training or were sedentary. Both lines’ muscles and plasma were analyzed. Muscle RNA was sequenced, and KEGG pathway analysis was performed. Analyses of muscle revealed no significant selection-related differences in muscle structure. However, in response to physical exercise, glucose and fatty acid oxidation were stimulated, lactate dehydrogenase activity was reduced, and lactate formation was inhibited in the marathon mice compared with trained control mice. The lack of lactate formation in response to exercise appears to be associated with increased lipid mobilization from peripheral adipose tissue in DUhTP mice, suggesting a specific benefit of lactate avoidance. Thus, results from the analysis of muscle metabolism in born marathon mice, shaped by 35 years (140 generations) of phenotype selection for superior running performance, suggest increased metabolic flexibility in male marathon mice toward lipid catabolism regulated by lactate dehydrogenase. MDPI 2023-07-25 /pmc/articles/PMC10417281/ /pubmed/37566003 http://dx.doi.org/10.3390/cells12151925 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brenmoehl, Julia
Brosig, Elli
Trakooljul, Nares
Walz, Christina
Ohde, Daniela
Noce, Antonia
Walz, Michael
Langhammer, Martina
Petkov, Stefan
Röntgen, Monika
Maak, Steffen
Galuska, Christina E.
Fuchs, Beate
Kuhla, Björn
Ponsuksili, Siriluck
Wimmers, Klaus
Hoeflich, Andreas
Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title_full Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title_fullStr Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title_full_unstemmed Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title_short Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)—A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
title_sort metabolic pathway modeling in muscle of male marathon mice (duhtp) and controls (duc)—a possible role of lactate dehydrogenase in metabolic flexibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417281/
https://www.ncbi.nlm.nih.gov/pubmed/37566003
http://dx.doi.org/10.3390/cells12151925
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