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Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept

SIMPLE SUMMARY: Hypoxia is a phenomenon common in cervical cancer. Both the presence and function of CAFs are upregulated in a hypoxic environment. A key factor in the physiological response to hypoxia is hypoxia-inducible factor-1alpha (HIF-1 [Formula: see text]). We hypothesized that [(68)Ga]Ga-FA...

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Autores principales: Mokoala, Kgomotso M. G., Lawal, Ismaheel O., Maserumule, Letjie C., Bida, Meshack, Maes, Alex, Ndlovu, Honest, Reed, Janet, Mahapane, Johncy, Davis, Cindy, Van de Wiele, Christophe, Popoola, Gbenga, Giesel, Frederik L., Vorster, Mariza, Sathekge, Mike M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417683/
https://www.ncbi.nlm.nih.gov/pubmed/37568769
http://dx.doi.org/10.3390/cancers15153953
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author Mokoala, Kgomotso M. G.
Lawal, Ismaheel O.
Maserumule, Letjie C.
Bida, Meshack
Maes, Alex
Ndlovu, Honest
Reed, Janet
Mahapane, Johncy
Davis, Cindy
Van de Wiele, Christophe
Popoola, Gbenga
Giesel, Frederik L.
Vorster, Mariza
Sathekge, Mike M.
author_facet Mokoala, Kgomotso M. G.
Lawal, Ismaheel O.
Maserumule, Letjie C.
Bida, Meshack
Maes, Alex
Ndlovu, Honest
Reed, Janet
Mahapane, Johncy
Davis, Cindy
Van de Wiele, Christophe
Popoola, Gbenga
Giesel, Frederik L.
Vorster, Mariza
Sathekge, Mike M.
author_sort Mokoala, Kgomotso M. G.
collection PubMed
description SIMPLE SUMMARY: Hypoxia is a phenomenon common in cervical cancer. Both the presence and function of CAFs are upregulated in a hypoxic environment. A key factor in the physiological response to hypoxia is hypoxia-inducible factor-1alpha (HIF-1 [Formula: see text]). We hypothesized that [(68)Ga]Ga-FAPI PET may be used as an indirect tracer for mapping hypoxia by correlating the image findings to pathological analysis of HIF-1 [Formula: see text] expression. The maximum and mean standardized uptake value (SUVmax and SUVmean) and FAPI tumor volume (FAPI-TV) were documented. There was uptake of tracer in the pelvis (cervix region) in all patients studied. All patients had lymph node metastases, while only six patients had distant visceral or skeletal metastases. The average FAPI-TV for patients with additional sites of metastases was higher than those without. Immunohistochemistry revealed varying intensities of HIF-1 [Formula: see text] expression in all tested samples. The presence of skeletal metastasis was correlated to the HIF-1⍺ staining (percentage distribution). Furthermore, the FAPI-TV was a better predictor of metastatic disease than the SUVmax. ABSTRACT: Hypoxia leads to changes in tumor microenvironment (upregulated CAFs) with resultant aggressiveness. A key factor in the physiological response to hypoxia is hypoxia-inducible factor-1alpha (HIF-1 [Formula: see text]). [(68)Ga]Ga-FAPI PET imaging has been demonstrated in various cancer types. We hypothesized that [(68)Ga]Ga-FAPI PET may be used as an indirect tracer for mapping hypoxia by correlating the image findings to pathological analysis of HIF-1 [Formula: see text] expression. The [(68)Ga]Ga-FAPI PET/CT scans of women with cancer of the cervix were reviewed and the maximum and mean standardized uptake value (SUVmax and SUVmean) and FAPI tumor volume (FAPI-TV) were documented. Correlation analysis was performed between PET-derived parameters and immunohistochemical staining as well as between PET-derived parameters and the presence of metastasis. Ten women were included. All patients demonstrated tracer uptake in the primary site or region of the primary. All patients had lymph node metastases while only six patients had distant visceral or skeletal metastases. The mean SUVmax, SUVmean, and FAPI-TV was 18.89, 6.88, and 195.66 cm(3), respectively. The average FAPI-TV for patients with additional sites of metastases was higher than those without. Immunohistochemistry revealed varying intensities of HIF-1 [Formula: see text] expression in all tested samples. There was a positive correlation between the presence of skeletal metastases and staining for HIF-1 [Formula: see text]. The presence of skeletal metastasis was correlated to the HIF-1⍺ staining (percentage distribution). Furthermore, the FAPI-TV was a better predictor of metastatic disease than the SUVmax.
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spelling pubmed-104176832023-08-12 Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept Mokoala, Kgomotso M. G. Lawal, Ismaheel O. Maserumule, Letjie C. Bida, Meshack Maes, Alex Ndlovu, Honest Reed, Janet Mahapane, Johncy Davis, Cindy Van de Wiele, Christophe Popoola, Gbenga Giesel, Frederik L. Vorster, Mariza Sathekge, Mike M. Cancers (Basel) Article SIMPLE SUMMARY: Hypoxia is a phenomenon common in cervical cancer. Both the presence and function of CAFs are upregulated in a hypoxic environment. A key factor in the physiological response to hypoxia is hypoxia-inducible factor-1alpha (HIF-1 [Formula: see text]). We hypothesized that [(68)Ga]Ga-FAPI PET may be used as an indirect tracer for mapping hypoxia by correlating the image findings to pathological analysis of HIF-1 [Formula: see text] expression. The maximum and mean standardized uptake value (SUVmax and SUVmean) and FAPI tumor volume (FAPI-TV) were documented. There was uptake of tracer in the pelvis (cervix region) in all patients studied. All patients had lymph node metastases, while only six patients had distant visceral or skeletal metastases. The average FAPI-TV for patients with additional sites of metastases was higher than those without. Immunohistochemistry revealed varying intensities of HIF-1 [Formula: see text] expression in all tested samples. The presence of skeletal metastasis was correlated to the HIF-1⍺ staining (percentage distribution). Furthermore, the FAPI-TV was a better predictor of metastatic disease than the SUVmax. ABSTRACT: Hypoxia leads to changes in tumor microenvironment (upregulated CAFs) with resultant aggressiveness. A key factor in the physiological response to hypoxia is hypoxia-inducible factor-1alpha (HIF-1 [Formula: see text]). [(68)Ga]Ga-FAPI PET imaging has been demonstrated in various cancer types. We hypothesized that [(68)Ga]Ga-FAPI PET may be used as an indirect tracer for mapping hypoxia by correlating the image findings to pathological analysis of HIF-1 [Formula: see text] expression. The [(68)Ga]Ga-FAPI PET/CT scans of women with cancer of the cervix were reviewed and the maximum and mean standardized uptake value (SUVmax and SUVmean) and FAPI tumor volume (FAPI-TV) were documented. Correlation analysis was performed between PET-derived parameters and immunohistochemical staining as well as between PET-derived parameters and the presence of metastasis. Ten women were included. All patients demonstrated tracer uptake in the primary site or region of the primary. All patients had lymph node metastases while only six patients had distant visceral or skeletal metastases. The mean SUVmax, SUVmean, and FAPI-TV was 18.89, 6.88, and 195.66 cm(3), respectively. The average FAPI-TV for patients with additional sites of metastases was higher than those without. Immunohistochemistry revealed varying intensities of HIF-1 [Formula: see text] expression in all tested samples. There was a positive correlation between the presence of skeletal metastases and staining for HIF-1 [Formula: see text]. The presence of skeletal metastasis was correlated to the HIF-1⍺ staining (percentage distribution). Furthermore, the FAPI-TV was a better predictor of metastatic disease than the SUVmax. MDPI 2023-08-03 /pmc/articles/PMC10417683/ /pubmed/37568769 http://dx.doi.org/10.3390/cancers15153953 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mokoala, Kgomotso M. G.
Lawal, Ismaheel O.
Maserumule, Letjie C.
Bida, Meshack
Maes, Alex
Ndlovu, Honest
Reed, Janet
Mahapane, Johncy
Davis, Cindy
Van de Wiele, Christophe
Popoola, Gbenga
Giesel, Frederik L.
Vorster, Mariza
Sathekge, Mike M.
Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title_full Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title_fullStr Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title_full_unstemmed Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title_short Correlation between [(68)Ga]Ga-FAPI-46 PET Imaging and HIF-1α Immunohistochemical Analysis in Cervical Cancer: Proof-of-Concept
title_sort correlation between [(68)ga]ga-fapi-46 pet imaging and hif-1α immunohistochemical analysis in cervical cancer: proof-of-concept
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417683/
https://www.ncbi.nlm.nih.gov/pubmed/37568769
http://dx.doi.org/10.3390/cancers15153953
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