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Cfdp1 Is Essential for Cardiac Development and Function
Cardiovascular diseases (CVDs) are the prevalent cause of mortality worldwide. A combination of environmental and genetic effectors modulates the risk of developing them. Thus, it is vital to identify candidate genes and elucidate their role in the manifestation of the disease. Large-scale human stu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417793/ https://www.ncbi.nlm.nih.gov/pubmed/37566073 http://dx.doi.org/10.3390/cells12151994 |
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author | Giardoglou, Panagiota Deloukas, Panos Dedoussis, George Beis, Dimitris |
author_facet | Giardoglou, Panagiota Deloukas, Panos Dedoussis, George Beis, Dimitris |
author_sort | Giardoglou, Panagiota |
collection | PubMed |
description | Cardiovascular diseases (CVDs) are the prevalent cause of mortality worldwide. A combination of environmental and genetic effectors modulates the risk of developing them. Thus, it is vital to identify candidate genes and elucidate their role in the manifestation of the disease. Large-scale human studies have revealed the implication of Craniofacial Development Protein 1 (CFDP1) in Coronary Artery Disease (CAD). CFDP1 belongs to the evolutionary conserved Bucentaur (BCNT) family, and to date, its function and mechanism of action in Cardiovascular Development are still unclear. We utilized zebrafish to investigate the role of cfdp1 in the developing heart due to the high genomic homology, similarity in heart physiology, and ease of experimental manipulations. We showed that cfdp1 was expressed during development, and we tested two morpholinos and generated a cfdp1 mutant line. The cfdp1(−/−) embryos developed arrhythmic hearts and exhibited defective cardiac performance, which led to a lethal phenotype. Findings from both knockdown and knockout experiments showed that abrogation of cfdp1 leads to downregulation of Wnt signaling in embryonic hearts during valve development but without affecting Notch activation in this process. The cfdp1 zebrafish mutant line provides a valuable tool for unveiling the novel mechanism of regulating cardiac physiology and function. cfdp1 is essential for cardiac development, a previously unreported phenotype most likely due to early lethality in mice. The detected phenotype of bradycardia and arrhythmias is an observation with potential clinical relevance for humans carrying heterozygous CFDP1 mutations and their risk of developing CAD. |
format | Online Article Text |
id | pubmed-10417793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104177932023-08-12 Cfdp1 Is Essential for Cardiac Development and Function Giardoglou, Panagiota Deloukas, Panos Dedoussis, George Beis, Dimitris Cells Article Cardiovascular diseases (CVDs) are the prevalent cause of mortality worldwide. A combination of environmental and genetic effectors modulates the risk of developing them. Thus, it is vital to identify candidate genes and elucidate their role in the manifestation of the disease. Large-scale human studies have revealed the implication of Craniofacial Development Protein 1 (CFDP1) in Coronary Artery Disease (CAD). CFDP1 belongs to the evolutionary conserved Bucentaur (BCNT) family, and to date, its function and mechanism of action in Cardiovascular Development are still unclear. We utilized zebrafish to investigate the role of cfdp1 in the developing heart due to the high genomic homology, similarity in heart physiology, and ease of experimental manipulations. We showed that cfdp1 was expressed during development, and we tested two morpholinos and generated a cfdp1 mutant line. The cfdp1(−/−) embryos developed arrhythmic hearts and exhibited defective cardiac performance, which led to a lethal phenotype. Findings from both knockdown and knockout experiments showed that abrogation of cfdp1 leads to downregulation of Wnt signaling in embryonic hearts during valve development but without affecting Notch activation in this process. The cfdp1 zebrafish mutant line provides a valuable tool for unveiling the novel mechanism of regulating cardiac physiology and function. cfdp1 is essential for cardiac development, a previously unreported phenotype most likely due to early lethality in mice. The detected phenotype of bradycardia and arrhythmias is an observation with potential clinical relevance for humans carrying heterozygous CFDP1 mutations and their risk of developing CAD. MDPI 2023-08-03 /pmc/articles/PMC10417793/ /pubmed/37566073 http://dx.doi.org/10.3390/cells12151994 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Giardoglou, Panagiota Deloukas, Panos Dedoussis, George Beis, Dimitris Cfdp1 Is Essential for Cardiac Development and Function |
title | Cfdp1 Is Essential for Cardiac Development and Function |
title_full | Cfdp1 Is Essential for Cardiac Development and Function |
title_fullStr | Cfdp1 Is Essential for Cardiac Development and Function |
title_full_unstemmed | Cfdp1 Is Essential for Cardiac Development and Function |
title_short | Cfdp1 Is Essential for Cardiac Development and Function |
title_sort | cfdp1 is essential for cardiac development and function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417793/ https://www.ncbi.nlm.nih.gov/pubmed/37566073 http://dx.doi.org/10.3390/cells12151994 |
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