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A robust method for measuring aminoacylation through tRNA-Seq

Current methods to quantify the fraction of aminoacylated tRNAs, also known as the tRNA charge, are limited by issues with either low throughput, precision, and/or accuracy. Here, we present an optimized charge tRNA-Seq method that combines previous developments with newly described approaches to es...

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Detalles Bibliográficos
Autores principales: Davidsen, Kristian, Sullivan, Lucas B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418082/
https://www.ncbi.nlm.nih.gov/pubmed/37577502
http://dx.doi.org/10.1101/2023.07.31.551363