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The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method

In this study, liposomes enclosing eugenol were prepared using microfluidics. Two lipids—1,2-dimyristoyl-sn-glycero-3-phosphocholine, 18:0 (DSPC) and 2-dimyristoyl-sn-glycero-3-phosphocholine, 14:0 (DMPC)—and microfluidic chips with serpentine and Y-shaped micromixing designs were used for the lipos...

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Autores principales: Ghodke, Jessica, Ekonomou, Sotirios I., Weaver, Edward, Lamprou, Dimitrios, Doran, Olena, Stratakos, Alexandros Ch.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418319/
https://www.ncbi.nlm.nih.gov/pubmed/37569209
http://dx.doi.org/10.3390/foods12152940
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author Ghodke, Jessica
Ekonomou, Sotirios I.
Weaver, Edward
Lamprou, Dimitrios
Doran, Olena
Stratakos, Alexandros Ch.
author_facet Ghodke, Jessica
Ekonomou, Sotirios I.
Weaver, Edward
Lamprou, Dimitrios
Doran, Olena
Stratakos, Alexandros Ch.
author_sort Ghodke, Jessica
collection PubMed
description In this study, liposomes enclosing eugenol were prepared using microfluidics. Two lipids—1,2-dimyristoyl-sn-glycero-3-phosphocholine, 18:0 (DSPC) and 2-dimyristoyl-sn-glycero-3-phosphocholine, 14:0 (DMPC)—and microfluidic chips with serpentine and Y-shaped micromixing designs were used for the liposomal formulation. Minimum bactericidal concentration (MBC) values indicated that eugenol was more effective against Gram-negative than Gram-positive bacteria. Four different flow-rate ratios (FRR 2:1, 3:1, 4:1, 5:1) were explored. All liposomes’ encapsulation efficiency (EE) was determined: 94.34% for DSPC 3:1 and 78.63% for DMPC 5:1. The highest eugenol release of 99.86% was observed at pH 4, DMPC 3:1 (Y-shaped chip). Liposomes were physically stable at 4, 20 and 37 °C for 60 days as determined by their size, polydispersity index (PDI) and zeta potential (ZP). The most stable liposomes were observed at FRR 5:1 for DSPC. EE, stability, and eugenol release studies proved that the liposomal formulations produced can be used as delivery vehicles to increase food safety.
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spelling pubmed-104183192023-08-12 The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method Ghodke, Jessica Ekonomou, Sotirios I. Weaver, Edward Lamprou, Dimitrios Doran, Olena Stratakos, Alexandros Ch. Foods Article In this study, liposomes enclosing eugenol were prepared using microfluidics. Two lipids—1,2-dimyristoyl-sn-glycero-3-phosphocholine, 18:0 (DSPC) and 2-dimyristoyl-sn-glycero-3-phosphocholine, 14:0 (DMPC)—and microfluidic chips with serpentine and Y-shaped micromixing designs were used for the liposomal formulation. Minimum bactericidal concentration (MBC) values indicated that eugenol was more effective against Gram-negative than Gram-positive bacteria. Four different flow-rate ratios (FRR 2:1, 3:1, 4:1, 5:1) were explored. All liposomes’ encapsulation efficiency (EE) was determined: 94.34% for DSPC 3:1 and 78.63% for DMPC 5:1. The highest eugenol release of 99.86% was observed at pH 4, DMPC 3:1 (Y-shaped chip). Liposomes were physically stable at 4, 20 and 37 °C for 60 days as determined by their size, polydispersity index (PDI) and zeta potential (ZP). The most stable liposomes were observed at FRR 5:1 for DSPC. EE, stability, and eugenol release studies proved that the liposomal formulations produced can be used as delivery vehicles to increase food safety. MDPI 2023-08-03 /pmc/articles/PMC10418319/ /pubmed/37569209 http://dx.doi.org/10.3390/foods12152940 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghodke, Jessica
Ekonomou, Sotirios I.
Weaver, Edward
Lamprou, Dimitrios
Doran, Olena
Stratakos, Alexandros Ch.
The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title_full The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title_fullStr The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title_full_unstemmed The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title_short The Manufacturing and Characterisation of Eugenol-Enclosed Liposomes Produced by Microfluidic Method
title_sort manufacturing and characterisation of eugenol-enclosed liposomes produced by microfluidic method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418319/
https://www.ncbi.nlm.nih.gov/pubmed/37569209
http://dx.doi.org/10.3390/foods12152940
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