Cargando…

Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy

Autophagy is a catabolic pathway capable of degrading cellular components ranging from individual molecules to organelles. Autophagy helps cells cope with stress by removing superfluous or hazardous material. In a previous work, we demonstrated that transcriptional upregulation of two autophagy-rela...

Descripción completa

Detalles Bibliográficos
Autores principales: Elander, Pernilla H., Holla, Sanjana, Sabljić, Igor, Gutierrez-Beltran, Emilio, Willems, Patrick, Bozhkov, Peter V., Minina, Elena A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418956/
https://www.ncbi.nlm.nih.gov/pubmed/37569688
http://dx.doi.org/10.3390/ijms241512300
_version_ 1785088393575661568
author Elander, Pernilla H.
Holla, Sanjana
Sabljić, Igor
Gutierrez-Beltran, Emilio
Willems, Patrick
Bozhkov, Peter V.
Minina, Elena A.
author_facet Elander, Pernilla H.
Holla, Sanjana
Sabljić, Igor
Gutierrez-Beltran, Emilio
Willems, Patrick
Bozhkov, Peter V.
Minina, Elena A.
author_sort Elander, Pernilla H.
collection PubMed
description Autophagy is a catabolic pathway capable of degrading cellular components ranging from individual molecules to organelles. Autophagy helps cells cope with stress by removing superfluous or hazardous material. In a previous work, we demonstrated that transcriptional upregulation of two autophagy-related genes, ATG5 and ATG7, in Arabidopsis thaliana positively affected agronomically important traits: biomass, seed yield, tolerance to pathogens and oxidative stress. Although the occurrence of these traits correlated with enhanced autophagic activity, it is possible that autophagy-independent roles of ATG5 and ATG7 also contributed to the phenotypes. In this study, we employed affinity purification and LC-MS/MS to identify the interactome of wild-type ATG5 and its autophagy-inactive substitution mutant, ATG5(K128R) Here we present the first interactome of plant ATG5, encompassing not only known autophagy regulators but also stress-response factors, components of the ubiquitin-proteasome system, proteins involved in endomembrane trafficking, and potential partners of the nuclear fraction of ATG5. Furthermore, we discovered post-translational modifications, such as phosphorylation and acetylation present on ATG5 complex components that are likely to play regulatory functions. These results strongly indicate that plant ATG5 complex proteins have roles beyond autophagy itself, opening avenues for further investigations on the complex roles of autophagy in plant growth and stress responses.
format Online
Article
Text
id pubmed-10418956
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104189562023-08-12 Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy Elander, Pernilla H. Holla, Sanjana Sabljić, Igor Gutierrez-Beltran, Emilio Willems, Patrick Bozhkov, Peter V. Minina, Elena A. Int J Mol Sci Article Autophagy is a catabolic pathway capable of degrading cellular components ranging from individual molecules to organelles. Autophagy helps cells cope with stress by removing superfluous or hazardous material. In a previous work, we demonstrated that transcriptional upregulation of two autophagy-related genes, ATG5 and ATG7, in Arabidopsis thaliana positively affected agronomically important traits: biomass, seed yield, tolerance to pathogens and oxidative stress. Although the occurrence of these traits correlated with enhanced autophagic activity, it is possible that autophagy-independent roles of ATG5 and ATG7 also contributed to the phenotypes. In this study, we employed affinity purification and LC-MS/MS to identify the interactome of wild-type ATG5 and its autophagy-inactive substitution mutant, ATG5(K128R) Here we present the first interactome of plant ATG5, encompassing not only known autophagy regulators but also stress-response factors, components of the ubiquitin-proteasome system, proteins involved in endomembrane trafficking, and potential partners of the nuclear fraction of ATG5. Furthermore, we discovered post-translational modifications, such as phosphorylation and acetylation present on ATG5 complex components that are likely to play regulatory functions. These results strongly indicate that plant ATG5 complex proteins have roles beyond autophagy itself, opening avenues for further investigations on the complex roles of autophagy in plant growth and stress responses. MDPI 2023-08-01 /pmc/articles/PMC10418956/ /pubmed/37569688 http://dx.doi.org/10.3390/ijms241512300 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elander, Pernilla H.
Holla, Sanjana
Sabljić, Igor
Gutierrez-Beltran, Emilio
Willems, Patrick
Bozhkov, Peter V.
Minina, Elena A.
Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title_full Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title_fullStr Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title_full_unstemmed Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title_short Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
title_sort interactome of arabidopsis atg5 suggests functions beyond autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418956/
https://www.ncbi.nlm.nih.gov/pubmed/37569688
http://dx.doi.org/10.3390/ijms241512300
work_keys_str_mv AT elanderpernillah interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT hollasanjana interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT sabljicigor interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT gutierrezbeltranemilio interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT willemspatrick interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT bozhkovpeterv interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy
AT mininaelenaa interactomeofarabidopsisatg5suggestsfunctionsbeyondautophagy