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Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells

The cell membrane is an important regulator for the cytotoxicity of chemotherapeutic agents. However, the biochemical and biophysical effects that occur in the membrane under the action of chemotherapy drugs are not fully described. In the present study, changes in the microviscosity of membranes of...

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Autores principales: Shimolina, Liubov, Gulin, Alexander, Khlynova, Alexandra, Ignatova, Nadezhda, Druzhkova, Irina, Gubina, Margarita, Zagaynova, Elena, Kuimova, Marina K., Shirmanova, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419023/
https://www.ncbi.nlm.nih.gov/pubmed/37569560
http://dx.doi.org/10.3390/ijms241512186
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author Shimolina, Liubov
Gulin, Alexander
Khlynova, Alexandra
Ignatova, Nadezhda
Druzhkova, Irina
Gubina, Margarita
Zagaynova, Elena
Kuimova, Marina K.
Shirmanova, Marina
author_facet Shimolina, Liubov
Gulin, Alexander
Khlynova, Alexandra
Ignatova, Nadezhda
Druzhkova, Irina
Gubina, Margarita
Zagaynova, Elena
Kuimova, Marina K.
Shirmanova, Marina
author_sort Shimolina, Liubov
collection PubMed
description The cell membrane is an important regulator for the cytotoxicity of chemotherapeutic agents. However, the biochemical and biophysical effects that occur in the membrane under the action of chemotherapy drugs are not fully described. In the present study, changes in the microviscosity of membranes of living HeLa–Kyoto tumor cells were studied during chemotherapy with paclitaxel, a widely used antimicrotubule agent. To visualize the microviscosity of the membranes, fluorescence lifetime imaging microscopy (FLIM) with a BODIPY 2 fluorescent molecular rotor was used. The lipid profile of the membranes was assessed using time-of-flight secondary ion mass spectrometry ToF-SIMS. A significant, steady-state decrease in the microviscosity of membranes, both in cell monolayers and in tumor spheroids, was revealed after the treatment. Mass spectrometry showed an increase in the unsaturated fatty acid content in treated cell membranes, which may explain, at least partially, their low microviscosity. These results indicate the involvement of membrane microviscosity in the response of tumor cells to paclitaxel treatment.
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spelling pubmed-104190232023-08-12 Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells Shimolina, Liubov Gulin, Alexander Khlynova, Alexandra Ignatova, Nadezhda Druzhkova, Irina Gubina, Margarita Zagaynova, Elena Kuimova, Marina K. Shirmanova, Marina Int J Mol Sci Article The cell membrane is an important regulator for the cytotoxicity of chemotherapeutic agents. However, the biochemical and biophysical effects that occur in the membrane under the action of chemotherapy drugs are not fully described. In the present study, changes in the microviscosity of membranes of living HeLa–Kyoto tumor cells were studied during chemotherapy with paclitaxel, a widely used antimicrotubule agent. To visualize the microviscosity of the membranes, fluorescence lifetime imaging microscopy (FLIM) with a BODIPY 2 fluorescent molecular rotor was used. The lipid profile of the membranes was assessed using time-of-flight secondary ion mass spectrometry ToF-SIMS. A significant, steady-state decrease in the microviscosity of membranes, both in cell monolayers and in tumor spheroids, was revealed after the treatment. Mass spectrometry showed an increase in the unsaturated fatty acid content in treated cell membranes, which may explain, at least partially, their low microviscosity. These results indicate the involvement of membrane microviscosity in the response of tumor cells to paclitaxel treatment. MDPI 2023-07-29 /pmc/articles/PMC10419023/ /pubmed/37569560 http://dx.doi.org/10.3390/ijms241512186 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimolina, Liubov
Gulin, Alexander
Khlynova, Alexandra
Ignatova, Nadezhda
Druzhkova, Irina
Gubina, Margarita
Zagaynova, Elena
Kuimova, Marina K.
Shirmanova, Marina
Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title_full Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title_fullStr Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title_full_unstemmed Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title_short Effects of Paclitaxel on Plasma Membrane Microviscosity and Lipid Composition in Cancer Cells
title_sort effects of paclitaxel on plasma membrane microviscosity and lipid composition in cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419023/
https://www.ncbi.nlm.nih.gov/pubmed/37569560
http://dx.doi.org/10.3390/ijms241512186
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