Cargando…

Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity

Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MP...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Aden Geonhee, Kang, Sora, Yoon, Hye Ji, Im, Suyeol, Oh, Seung Jun, Pak, Youngmi Kim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419071/
https://www.ncbi.nlm.nih.gov/pubmed/37569796
http://dx.doi.org/10.3390/ijms241512421
_version_ 1785088422653722624
author Lee, Aden Geonhee
Kang, Sora
Yoon, Hye Ji
Im, Suyeol
Oh, Seung Jun
Pak, Youngmi Kim
author_facet Lee, Aden Geonhee
Kang, Sora
Yoon, Hye Ji
Im, Suyeol
Oh, Seung Jun
Pak, Youngmi Kim
author_sort Lee, Aden Geonhee
collection PubMed
description Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MPs. In this study, we conducted a comprehensive investigation utilizing in silico, in vitro, and in vivo approaches to explore the brain distribution and physiological effects of MPs. Molecular docking simulations were performed to assess the binding affinity of three plastic polymers (ethylene, propylene, and styrene) to immune cells (macrophages, CD4(+), and CD8(+) lymphocytes). The results revealed that styrene exhibited the highest binding affinity for macrophages. Furthermore, in vitro experiments employing fluorescence-labeled PS-MPs (fPS-MPs) of 1 μm at various concentrations demonstrated a dose-dependent binding of fPS-MPs to BV2 murine microglial cells. Subsequent oral administration of fPS-MPs to high-fat diet-induced obese mice led to the co-existence of fPS-MPs with immune cells in the blood, exacerbating impaired glucose metabolism and insulin resistance and promoting systemic inflammation. Additionally, fPS-MPs were detected throughout the brain, with increased activation of microglia in the hypothalamus. These findings suggest that PS-MPs significantly contribute to the exacerbation of systemic inflammation in high-fat diet-induced obesity by activating peripheral and central inflammatory immune cells.
format Online
Article
Text
id pubmed-10419071
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104190712023-08-12 Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity Lee, Aden Geonhee Kang, Sora Yoon, Hye Ji Im, Suyeol Oh, Seung Jun Pak, Youngmi Kim Int J Mol Sci Article Microplastics (MPs) are recognized as environmental pollutants with potential implications for human health. Considering the rapid increase in obesity rates despite stable caloric intake, there is a growing concern about the link between obesity and exposure to environmental pollutants, including MPs. In this study, we conducted a comprehensive investigation utilizing in silico, in vitro, and in vivo approaches to explore the brain distribution and physiological effects of MPs. Molecular docking simulations were performed to assess the binding affinity of three plastic polymers (ethylene, propylene, and styrene) to immune cells (macrophages, CD4(+), and CD8(+) lymphocytes). The results revealed that styrene exhibited the highest binding affinity for macrophages. Furthermore, in vitro experiments employing fluorescence-labeled PS-MPs (fPS-MPs) of 1 μm at various concentrations demonstrated a dose-dependent binding of fPS-MPs to BV2 murine microglial cells. Subsequent oral administration of fPS-MPs to high-fat diet-induced obese mice led to the co-existence of fPS-MPs with immune cells in the blood, exacerbating impaired glucose metabolism and insulin resistance and promoting systemic inflammation. Additionally, fPS-MPs were detected throughout the brain, with increased activation of microglia in the hypothalamus. These findings suggest that PS-MPs significantly contribute to the exacerbation of systemic inflammation in high-fat diet-induced obesity by activating peripheral and central inflammatory immune cells. MDPI 2023-08-04 /pmc/articles/PMC10419071/ /pubmed/37569796 http://dx.doi.org/10.3390/ijms241512421 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Aden Geonhee
Kang, Sora
Yoon, Hye Ji
Im, Suyeol
Oh, Seung Jun
Pak, Youngmi Kim
Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title_full Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title_fullStr Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title_full_unstemmed Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title_short Polystyrene Microplastics Exacerbate Systemic Inflammation in High-Fat Diet-Induced Obesity
title_sort polystyrene microplastics exacerbate systemic inflammation in high-fat diet-induced obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419071/
https://www.ncbi.nlm.nih.gov/pubmed/37569796
http://dx.doi.org/10.3390/ijms241512421
work_keys_str_mv AT leeadengeonhee polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity
AT kangsora polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity
AT yoonhyeji polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity
AT imsuyeol polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity
AT ohseungjun polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity
AT pakyoungmikim polystyrenemicroplasticsexacerbatesystemicinflammationinhighfatdietinducedobesity