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Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20

Brevibacillus sp. SPR20 produced potentially antibacterial substances against methicillin-resistant Staphylococcus aureus (MRSA). The synthesis of these substances is controlled by their biosynthetic gene clusters. Several mutagenesis methods are used to overcome the restriction of gene regulations...

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Autores principales: Songnaka, Nuttapon, Lertcanawanichakul, Monthon, Hutapea, Albert Manggading, Nisoa, Mudtorlep, Krobthong, Sucheewin, Yingchutrakul, Yodying, Atipairin, Apichart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419081/
https://www.ncbi.nlm.nih.gov/pubmed/37569391
http://dx.doi.org/10.3390/ijms241512016
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author Songnaka, Nuttapon
Lertcanawanichakul, Monthon
Hutapea, Albert Manggading
Nisoa, Mudtorlep
Krobthong, Sucheewin
Yingchutrakul, Yodying
Atipairin, Apichart
author_facet Songnaka, Nuttapon
Lertcanawanichakul, Monthon
Hutapea, Albert Manggading
Nisoa, Mudtorlep
Krobthong, Sucheewin
Yingchutrakul, Yodying
Atipairin, Apichart
author_sort Songnaka, Nuttapon
collection PubMed
description Brevibacillus sp. SPR20 produced potentially antibacterial substances against methicillin-resistant Staphylococcus aureus (MRSA). The synthesis of these substances is controlled by their biosynthetic gene clusters. Several mutagenesis methods are used to overcome the restriction of gene regulations when genetic information is absent. Atmospheric and room temperature plasma (ARTP) is a powerful technique to initiate random mutagenesis for microbial strain improvement. This study utilized an argon-based ARTP to conduct the mutations on SPR20. The positive mutants of 40% occurred. The M27 mutant exhibited an increase in anti-MRSA activity when compared to the wild-type strain, with the MIC values of 250–500 and 500 μg/mL, respectively. M27 had genetic stability because it exhibited constant activity throughout fifteen generations. This mutant had similar morphology and antibiotic susceptibility to the wild type. Comparative proteomic analysis identified some specific proteins that were upregulated in M27. These proteins were involved in the metabolism of amino acids, cell structure and movement, and catalytic enzymes. These might result in the enhancement of the anti-MRSA activity of the ARTP-treated SPR20 mutant. This study supports the ARTP technology designed to increase the production of valuable antibacterial agents.
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spelling pubmed-104190812023-08-12 Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20 Songnaka, Nuttapon Lertcanawanichakul, Monthon Hutapea, Albert Manggading Nisoa, Mudtorlep Krobthong, Sucheewin Yingchutrakul, Yodying Atipairin, Apichart Int J Mol Sci Article Brevibacillus sp. SPR20 produced potentially antibacterial substances against methicillin-resistant Staphylococcus aureus (MRSA). The synthesis of these substances is controlled by their biosynthetic gene clusters. Several mutagenesis methods are used to overcome the restriction of gene regulations when genetic information is absent. Atmospheric and room temperature plasma (ARTP) is a powerful technique to initiate random mutagenesis for microbial strain improvement. This study utilized an argon-based ARTP to conduct the mutations on SPR20. The positive mutants of 40% occurred. The M27 mutant exhibited an increase in anti-MRSA activity when compared to the wild-type strain, with the MIC values of 250–500 and 500 μg/mL, respectively. M27 had genetic stability because it exhibited constant activity throughout fifteen generations. This mutant had similar morphology and antibiotic susceptibility to the wild type. Comparative proteomic analysis identified some specific proteins that were upregulated in M27. These proteins were involved in the metabolism of amino acids, cell structure and movement, and catalytic enzymes. These might result in the enhancement of the anti-MRSA activity of the ARTP-treated SPR20 mutant. This study supports the ARTP technology designed to increase the production of valuable antibacterial agents. MDPI 2023-07-27 /pmc/articles/PMC10419081/ /pubmed/37569391 http://dx.doi.org/10.3390/ijms241512016 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Songnaka, Nuttapon
Lertcanawanichakul, Monthon
Hutapea, Albert Manggading
Nisoa, Mudtorlep
Krobthong, Sucheewin
Yingchutrakul, Yodying
Atipairin, Apichart
Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title_full Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title_fullStr Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title_full_unstemmed Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title_short Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20
title_sort atmospheric and room temperature plasma (artp) mutagenesis improved the anti-mrsa activity of brevibacillus sp. spr20
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419081/
https://www.ncbi.nlm.nih.gov/pubmed/37569391
http://dx.doi.org/10.3390/ijms241512016
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