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Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification

The yeast S. cerevisiae is a unique genetic object for which a wide range of relatively simple, inexpensive, and non-time-consuming methods have been developed that allow the performing of a wide variety of genome modifications. Among the latter, one can mention point mutations, disruptions and dele...

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Autores principales: Stepchenkova, Elena I., Zadorsky, Sergey P., Shumega, Andrey R., Aksenova, Anna Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419131/
https://www.ncbi.nlm.nih.gov/pubmed/37569333
http://dx.doi.org/10.3390/ijms241511960
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author Stepchenkova, Elena I.
Zadorsky, Sergey P.
Shumega, Andrey R.
Aksenova, Anna Y.
author_facet Stepchenkova, Elena I.
Zadorsky, Sergey P.
Shumega, Andrey R.
Aksenova, Anna Y.
author_sort Stepchenkova, Elena I.
collection PubMed
description The yeast S. cerevisiae is a unique genetic object for which a wide range of relatively simple, inexpensive, and non-time-consuming methods have been developed that allow the performing of a wide variety of genome modifications. Among the latter, one can mention point mutations, disruptions and deletions of particular genes and regions of chromosomes, insertion of cassettes for the expression of heterologous genes, targeted chromosomal rearrangements such as translocations and inversions, directed changes in the karyotype (loss or duplication of particular chromosomes, changes in the level of ploidy), mating-type changes, etc. Classical yeast genome manipulations have been advanced with CRISPR/Cas9 technology in recent years that allow for the generation of multiple simultaneous changes in the yeast genome. In this review we discuss practical applications of both the classical yeast genome modification methods as well as CRISPR/Cas9 technology. In addition, we review methods for ploidy changes, including aneuploid generation, methods for mating type switching and directed DSB. Combined with a description of useful selective markers and transformation techniques, this work represents a nearly complete guide to yeast genome modification.
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spelling pubmed-104191312023-08-12 Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification Stepchenkova, Elena I. Zadorsky, Sergey P. Shumega, Andrey R. Aksenova, Anna Y. Int J Mol Sci Review The yeast S. cerevisiae is a unique genetic object for which a wide range of relatively simple, inexpensive, and non-time-consuming methods have been developed that allow the performing of a wide variety of genome modifications. Among the latter, one can mention point mutations, disruptions and deletions of particular genes and regions of chromosomes, insertion of cassettes for the expression of heterologous genes, targeted chromosomal rearrangements such as translocations and inversions, directed changes in the karyotype (loss or duplication of particular chromosomes, changes in the level of ploidy), mating-type changes, etc. Classical yeast genome manipulations have been advanced with CRISPR/Cas9 technology in recent years that allow for the generation of multiple simultaneous changes in the yeast genome. In this review we discuss practical applications of both the classical yeast genome modification methods as well as CRISPR/Cas9 technology. In addition, we review methods for ploidy changes, including aneuploid generation, methods for mating type switching and directed DSB. Combined with a description of useful selective markers and transformation techniques, this work represents a nearly complete guide to yeast genome modification. MDPI 2023-07-26 /pmc/articles/PMC10419131/ /pubmed/37569333 http://dx.doi.org/10.3390/ijms241511960 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Stepchenkova, Elena I.
Zadorsky, Sergey P.
Shumega, Andrey R.
Aksenova, Anna Y.
Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title_full Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title_fullStr Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title_full_unstemmed Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title_short Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification
title_sort practical approaches for the yeast saccharomyces cerevisiae genome modification
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419131/
https://www.ncbi.nlm.nih.gov/pubmed/37569333
http://dx.doi.org/10.3390/ijms241511960
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