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Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice

Sepsis is a life-threatening condition that results from an overwhelming and disproportionate host response to an infection. Currently, the quality and extent of the immune response are evaluated based on clinical symptoms and the concentration of inflammatory biomarkers released or expressed by the...

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Autores principales: Osadare, Ibukun Elizabeth, Xiong, Ling, Rubio, Ignacio, Neugebauer, Ute, Press, Adrian T., Ramoji, Anuradha, Popp, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419286/
https://www.ncbi.nlm.nih.gov/pubmed/37569403
http://dx.doi.org/10.3390/ijms241512027
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author Osadare, Ibukun Elizabeth
Xiong, Ling
Rubio, Ignacio
Neugebauer, Ute
Press, Adrian T.
Ramoji, Anuradha
Popp, Juergen
author_facet Osadare, Ibukun Elizabeth
Xiong, Ling
Rubio, Ignacio
Neugebauer, Ute
Press, Adrian T.
Ramoji, Anuradha
Popp, Juergen
author_sort Osadare, Ibukun Elizabeth
collection PubMed
description Sepsis is a life-threatening condition that results from an overwhelming and disproportionate host response to an infection. Currently, the quality and extent of the immune response are evaluated based on clinical symptoms and the concentration of inflammatory biomarkers released or expressed by the immune cells. However, the host response toward sepsis is heterogeneous, and the roles of the individual immune cell types have not been fully conceptualized. During sepsis, the spleen plays a vital role in pathogen clearance, such as bacteria by an antibody response, macrophage bactericidal capacity, and bacterial endotoxin detoxification. This study uses Raman spectroscopy to understand the splenic T-lymphocyte compartment profile changes during bona fide bacterial sepsis versus hyperinflammatory endotoxemia. The Raman spectral analysis showed marked changes in splenocytes of mice subjected to septic peritonitis principally in the DNA region, with minor changes in the amino acids and lipoprotein areas, indicating significant transcriptomic activity during sepsis. Furthermore, splenocytes from mice exposed to endotoxic shock by injection of a high dose of lipopolysaccharide showed significant changes in the protein and lipid profiles, albeit with interindividual variations in inflammation severity. In summary, this study provided experimental evidence for the applicability and informative value of Raman spectroscopy for profiling the immune response in a complex, systemic infection scenario. Importantly, changes within the acute phase of inflammation onset (24 h) were reliably detected, lending support to the concept of early treatment and severity control by extracorporeal Raman profiling of immunocyte signatures.
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spelling pubmed-104192862023-08-12 Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice Osadare, Ibukun Elizabeth Xiong, Ling Rubio, Ignacio Neugebauer, Ute Press, Adrian T. Ramoji, Anuradha Popp, Juergen Int J Mol Sci Article Sepsis is a life-threatening condition that results from an overwhelming and disproportionate host response to an infection. Currently, the quality and extent of the immune response are evaluated based on clinical symptoms and the concentration of inflammatory biomarkers released or expressed by the immune cells. However, the host response toward sepsis is heterogeneous, and the roles of the individual immune cell types have not been fully conceptualized. During sepsis, the spleen plays a vital role in pathogen clearance, such as bacteria by an antibody response, macrophage bactericidal capacity, and bacterial endotoxin detoxification. This study uses Raman spectroscopy to understand the splenic T-lymphocyte compartment profile changes during bona fide bacterial sepsis versus hyperinflammatory endotoxemia. The Raman spectral analysis showed marked changes in splenocytes of mice subjected to septic peritonitis principally in the DNA region, with minor changes in the amino acids and lipoprotein areas, indicating significant transcriptomic activity during sepsis. Furthermore, splenocytes from mice exposed to endotoxic shock by injection of a high dose of lipopolysaccharide showed significant changes in the protein and lipid profiles, albeit with interindividual variations in inflammation severity. In summary, this study provided experimental evidence for the applicability and informative value of Raman spectroscopy for profiling the immune response in a complex, systemic infection scenario. Importantly, changes within the acute phase of inflammation onset (24 h) were reliably detected, lending support to the concept of early treatment and severity control by extracorporeal Raman profiling of immunocyte signatures. MDPI 2023-07-27 /pmc/articles/PMC10419286/ /pubmed/37569403 http://dx.doi.org/10.3390/ijms241512027 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Osadare, Ibukun Elizabeth
Xiong, Ling
Rubio, Ignacio
Neugebauer, Ute
Press, Adrian T.
Ramoji, Anuradha
Popp, Juergen
Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title_full Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title_fullStr Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title_full_unstemmed Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title_short Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice
title_sort raman spectroscopy profiling of splenic t-cells in sepsis and endotoxemia in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419286/
https://www.ncbi.nlm.nih.gov/pubmed/37569403
http://dx.doi.org/10.3390/ijms241512027
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