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Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns

Ferns and lycophytes have received scant molecular attention in comparison to angiosperms. The advent of high-throughput technologies allowed an advance towards a greater knowledge of their elusive genomes. In this work, proteomic analyses of heart-shaped gametophytes of two ferns were performed: th...

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Autores principales: Ojosnegros, Sara, Alvarez, José Manuel, Grossmann, Jonas, Gagliardini, Valeria, Quintanilla, Luis G., Grossniklaus, Ueli, Fernández, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419320/
https://www.ncbi.nlm.nih.gov/pubmed/37569809
http://dx.doi.org/10.3390/ijms241512429
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author Ojosnegros, Sara
Alvarez, José Manuel
Grossmann, Jonas
Gagliardini, Valeria
Quintanilla, Luis G.
Grossniklaus, Ueli
Fernández, Helena
author_facet Ojosnegros, Sara
Alvarez, José Manuel
Grossmann, Jonas
Gagliardini, Valeria
Quintanilla, Luis G.
Grossniklaus, Ueli
Fernández, Helena
author_sort Ojosnegros, Sara
collection PubMed
description Ferns and lycophytes have received scant molecular attention in comparison to angiosperms. The advent of high-throughput technologies allowed an advance towards a greater knowledge of their elusive genomes. In this work, proteomic analyses of heart-shaped gametophytes of two ferns were performed: the apomictic Dryopteris affinis ssp. affinis and its sexual relative Dryopteris oreades. In total, a set of 218 proteins shared by these two gametophytes were analyzed using the STRING database, and their proteome associated with metabolism, genetic information processing, and responses to abiotic stress is discussed. Specifically, we report proteins involved in the metabolism of carbohydrates, lipids, and nucleotides, the biosynthesis of amino acids and secondary compounds, energy, oxide-reduction, transcription, translation, protein folding, sorting and degradation, and responses to abiotic stresses. The interactome of this set of proteins represents a total network composed of 218 nodes and 1792 interactions, obtained mostly from databases and text mining. The interactions among the identified proteins of the ferns D. affinis and D. oreades, together with the description of their biological functions, might contribute to a better understanding of the function and development of ferns as well as fill knowledge gaps in plant evolution.
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spelling pubmed-104193202023-08-12 Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns Ojosnegros, Sara Alvarez, José Manuel Grossmann, Jonas Gagliardini, Valeria Quintanilla, Luis G. Grossniklaus, Ueli Fernández, Helena Int J Mol Sci Article Ferns and lycophytes have received scant molecular attention in comparison to angiosperms. The advent of high-throughput technologies allowed an advance towards a greater knowledge of their elusive genomes. In this work, proteomic analyses of heart-shaped gametophytes of two ferns were performed: the apomictic Dryopteris affinis ssp. affinis and its sexual relative Dryopteris oreades. In total, a set of 218 proteins shared by these two gametophytes were analyzed using the STRING database, and their proteome associated with metabolism, genetic information processing, and responses to abiotic stress is discussed. Specifically, we report proteins involved in the metabolism of carbohydrates, lipids, and nucleotides, the biosynthesis of amino acids and secondary compounds, energy, oxide-reduction, transcription, translation, protein folding, sorting and degradation, and responses to abiotic stresses. The interactome of this set of proteins represents a total network composed of 218 nodes and 1792 interactions, obtained mostly from databases and text mining. The interactions among the identified proteins of the ferns D. affinis and D. oreades, together with the description of their biological functions, might contribute to a better understanding of the function and development of ferns as well as fill knowledge gaps in plant evolution. MDPI 2023-08-04 /pmc/articles/PMC10419320/ /pubmed/37569809 http://dx.doi.org/10.3390/ijms241512429 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ojosnegros, Sara
Alvarez, José Manuel
Grossmann, Jonas
Gagliardini, Valeria
Quintanilla, Luis G.
Grossniklaus, Ueli
Fernández, Helena
Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title_full Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title_fullStr Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title_full_unstemmed Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title_short Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
title_sort proteome and interactome linked to metabolism, genetic information processing, and abiotic stress in gametophytes of two woodferns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419320/
https://www.ncbi.nlm.nih.gov/pubmed/37569809
http://dx.doi.org/10.3390/ijms241512429
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