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Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines
Human colostrum and milk contain diverse cells and soluble components that have the potential to act against tumors. In breast cancer, macrophages play a significant role in immune infiltration and contribute to the progression and spread of tumors. However, studies suggest that these cells can be r...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419558/ https://www.ncbi.nlm.nih.gov/pubmed/37569777 http://dx.doi.org/10.3390/ijms241512400 |
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author | Silva, Kenia Maria Rezende França, Danielle Cristina Honório de Queiroz, Adriele Ataídes Fagundes-Triches, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Morais, Tassiane Cristina Honorio-França, Adenilda Cristina França, Eduardo Luzía |
author_facet | Silva, Kenia Maria Rezende França, Danielle Cristina Honório de Queiroz, Adriele Ataídes Fagundes-Triches, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Morais, Tassiane Cristina Honorio-França, Adenilda Cristina França, Eduardo Luzía |
author_sort | Silva, Kenia Maria Rezende |
collection | PubMed |
description | Human colostrum and milk contain diverse cells and soluble components that have the potential to act against tumors. In breast cancer, macrophages play a significant role in immune infiltration and contribute to the progression and spread of tumors. However, studies suggest that these cells can be reprogrammed to act as an antitumor immune response. This study aimed to evaluate the levels of melatonin and its receptors, MT(1) (melatonin receptor 1) and MT(2) (melatonin receptor 2), in colostrum and assess the differentiation and polarization of the colostrum macrophages modulated by melatonin in the presence of breast tumor cells. Colostrum samples were collected from 116 mothers and tested for their melatonin and receptor levels. The colostrum cells were treated with or without melatonin and then cultured for 24 h in the presence or absence of breast tumor cells. The results showed that melatonin treatment increased the expression of MT(1) and MT(2) in the colostrum cells. Furthermore, melatonin treatment increased the percentage of M1 macrophages and decreased the percentage of M2 macrophages. When the colostrum macrophages were cocultured with breast tumor cells, melatonin reduced the percentage of both macrophage phenotypes and the cytokines tumor necrosis factor-alpha (TNF-α) and interleukin 8 (IL-8). These data suggest that melatonin can regulate the inflammatory process via M1 macrophages in the tumor microenvironment and, simultaneously, the progression of M2 macrophages that favor tumorigenesis. |
format | Online Article Text |
id | pubmed-10419558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104195582023-08-12 Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines Silva, Kenia Maria Rezende França, Danielle Cristina Honório de Queiroz, Adriele Ataídes Fagundes-Triches, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Morais, Tassiane Cristina Honorio-França, Adenilda Cristina França, Eduardo Luzía Int J Mol Sci Article Human colostrum and milk contain diverse cells and soluble components that have the potential to act against tumors. In breast cancer, macrophages play a significant role in immune infiltration and contribute to the progression and spread of tumors. However, studies suggest that these cells can be reprogrammed to act as an antitumor immune response. This study aimed to evaluate the levels of melatonin and its receptors, MT(1) (melatonin receptor 1) and MT(2) (melatonin receptor 2), in colostrum and assess the differentiation and polarization of the colostrum macrophages modulated by melatonin in the presence of breast tumor cells. Colostrum samples were collected from 116 mothers and tested for their melatonin and receptor levels. The colostrum cells were treated with or without melatonin and then cultured for 24 h in the presence or absence of breast tumor cells. The results showed that melatonin treatment increased the expression of MT(1) and MT(2) in the colostrum cells. Furthermore, melatonin treatment increased the percentage of M1 macrophages and decreased the percentage of M2 macrophages. When the colostrum macrophages were cocultured with breast tumor cells, melatonin reduced the percentage of both macrophage phenotypes and the cytokines tumor necrosis factor-alpha (TNF-α) and interleukin 8 (IL-8). These data suggest that melatonin can regulate the inflammatory process via M1 macrophages in the tumor microenvironment and, simultaneously, the progression of M2 macrophages that favor tumorigenesis. MDPI 2023-08-03 /pmc/articles/PMC10419558/ /pubmed/37569777 http://dx.doi.org/10.3390/ijms241512400 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Kenia Maria Rezende França, Danielle Cristina Honório de Queiroz, Adriele Ataídes Fagundes-Triches, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Morais, Tassiane Cristina Honorio-França, Adenilda Cristina França, Eduardo Luzía Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title | Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title_full | Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title_fullStr | Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title_full_unstemmed | Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title_short | Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines |
title_sort | polarization of melatonin-modulated colostrum macrophages in the presence of breast tumor cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419558/ https://www.ncbi.nlm.nih.gov/pubmed/37569777 http://dx.doi.org/10.3390/ijms241512400 |
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