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Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients
Rheumatoid arthritis (RA) is a complex, multifactorial disorder with an autoimmune etiology. RA is highly heritable and is associated with both human leucocyte antigen (HLA) and non-HLA genes. We investigated the associations of 33 single nucleotide polymorphisms (SNPs) with RA in the Saudi populati...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419658/ https://www.ncbi.nlm.nih.gov/pubmed/37568346 http://dx.doi.org/10.3390/jcm12154944 |
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author | Daghestani, Maha Othman, Nashwa Omair, Mohammed A. Alenzi, Fahidah Omair, Maha A. Alqurtas, Eman Amin, Shireen Warsy, Arjumand |
author_facet | Daghestani, Maha Othman, Nashwa Omair, Mohammed A. Alenzi, Fahidah Omair, Maha A. Alqurtas, Eman Amin, Shireen Warsy, Arjumand |
author_sort | Daghestani, Maha |
collection | PubMed |
description | Rheumatoid arthritis (RA) is a complex, multifactorial disorder with an autoimmune etiology. RA is highly heritable and is associated with both human leucocyte antigen (HLA) and non-HLA genes. We investigated the associations of 33 single nucleotide polymorphisms (SNPs) with RA in the Saudi population. Methods: This study included 105 patients with RA and an equal number of age- and sex-matched controls. The patients with RA attended outpatient clinics at King Khalid University Hospital in Riyadh, Saudi Arabia. Blood samples were collected, and DNA was extracted using Qiagen kits. Primers were designed for the 33 selected SNPs using the MassEXTEND primers program, and samples were genotyped on the Sequenom MassARRAY iPLEX platform. The allele frequencies and genotypes were determined for each SNP, and the results obtained for the patients were compared to those for the controls. Results: The allele and genotype frequencies of six SNPs were significantly associated with RA: rs1188934, rs10919563, rs3087243, rs1980422, rs10499194, and rs629326. The minor alleles of rs1188934, rs10919563, rs10499194, and rs629326 were protective, with odds ratios of 0.542, 0.597, 0.589, and 0.625, and p-values of 0.002, 0.023, 0.013 and 0.036, respectively. In addition, the heterozygote frequencies of two SNPs (rs6859219 and rs11586238) were significantly higher in the controls than in the patients. Conclusions: There is considerable heterogeneity in the genetics of RA in different populations, and the SNPs that are associated with RA in some populations are not in others. We studied 33 SNPs and only eight were associated with RA. The remaining SNPs showed no allelic or genotypic associations with RA. |
format | Online Article Text |
id | pubmed-10419658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104196582023-08-12 Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients Daghestani, Maha Othman, Nashwa Omair, Mohammed A. Alenzi, Fahidah Omair, Maha A. Alqurtas, Eman Amin, Shireen Warsy, Arjumand J Clin Med Article Rheumatoid arthritis (RA) is a complex, multifactorial disorder with an autoimmune etiology. RA is highly heritable and is associated with both human leucocyte antigen (HLA) and non-HLA genes. We investigated the associations of 33 single nucleotide polymorphisms (SNPs) with RA in the Saudi population. Methods: This study included 105 patients with RA and an equal number of age- and sex-matched controls. The patients with RA attended outpatient clinics at King Khalid University Hospital in Riyadh, Saudi Arabia. Blood samples were collected, and DNA was extracted using Qiagen kits. Primers were designed for the 33 selected SNPs using the MassEXTEND primers program, and samples were genotyped on the Sequenom MassARRAY iPLEX platform. The allele frequencies and genotypes were determined for each SNP, and the results obtained for the patients were compared to those for the controls. Results: The allele and genotype frequencies of six SNPs were significantly associated with RA: rs1188934, rs10919563, rs3087243, rs1980422, rs10499194, and rs629326. The minor alleles of rs1188934, rs10919563, rs10499194, and rs629326 were protective, with odds ratios of 0.542, 0.597, 0.589, and 0.625, and p-values of 0.002, 0.023, 0.013 and 0.036, respectively. In addition, the heterozygote frequencies of two SNPs (rs6859219 and rs11586238) were significantly higher in the controls than in the patients. Conclusions: There is considerable heterogeneity in the genetics of RA in different populations, and the SNPs that are associated with RA in some populations are not in others. We studied 33 SNPs and only eight were associated with RA. The remaining SNPs showed no allelic or genotypic associations with RA. MDPI 2023-07-27 /pmc/articles/PMC10419658/ /pubmed/37568346 http://dx.doi.org/10.3390/jcm12154944 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Daghestani, Maha Othman, Nashwa Omair, Mohammed A. Alenzi, Fahidah Omair, Maha A. Alqurtas, Eman Amin, Shireen Warsy, Arjumand Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title | Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title_full | Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title_fullStr | Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title_full_unstemmed | Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title_short | Single Nucleotide Polymorphisms Associated with Rheumatoid Arthritis in Saudi Patients |
title_sort | single nucleotide polymorphisms associated with rheumatoid arthritis in saudi patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419658/ https://www.ncbi.nlm.nih.gov/pubmed/37568346 http://dx.doi.org/10.3390/jcm12154944 |
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