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CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium

Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chr...

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Autores principales: Miao, Weihao, Xiao, Xiangyu, Wang, Yuean, Ge, Lijiao, Yang, Yanrong, Liu, Ye, Liao, Yuan, Guan, Zhiyong, Chen, Sumei, Fang, Weimin, Chen, Fadi, Zhao, Shuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419886/
https://www.ncbi.nlm.nih.gov/pubmed/37577400
http://dx.doi.org/10.1093/hr/uhad101
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author Miao, Weihao
Xiao, Xiangyu
Wang, Yuean
Ge, Lijiao
Yang, Yanrong
Liu, Ye
Liao, Yuan
Guan, Zhiyong
Chen, Sumei
Fang, Weimin
Chen, Fadi
Zhao, Shuang
author_facet Miao, Weihao
Xiao, Xiangyu
Wang, Yuean
Ge, Lijiao
Yang, Yanrong
Liu, Ye
Liao, Yuan
Guan, Zhiyong
Chen, Sumei
Fang, Weimin
Chen, Fadi
Zhao, Shuang
author_sort Miao, Weihao
collection PubMed
description Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chrysanthemum ‘Jinba’ and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that CmWRKY6–1 negatively regulated resistance to F. oxysporum and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression. Additionally, we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F. oxysporum via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F. oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system.
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spelling pubmed-104198862023-08-12 CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium Miao, Weihao Xiao, Xiangyu Wang, Yuean Ge, Lijiao Yang, Yanrong Liu, Ye Liao, Yuan Guan, Zhiyong Chen, Sumei Fang, Weimin Chen, Fadi Zhao, Shuang Hortic Res Article Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chrysanthemum ‘Jinba’ and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that CmWRKY6–1 negatively regulated resistance to F. oxysporum and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression. Additionally, we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F. oxysporum via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F. oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system. Oxford University Press 2023-05-10 /pmc/articles/PMC10419886/ /pubmed/37577400 http://dx.doi.org/10.1093/hr/uhad101 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Miao, Weihao
Xiao, Xiangyu
Wang, Yuean
Ge, Lijiao
Yang, Yanrong
Liu, Ye
Liao, Yuan
Guan, Zhiyong
Chen, Sumei
Fang, Weimin
Chen, Fadi
Zhao, Shuang
CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title_full CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title_fullStr CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title_full_unstemmed CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title_short CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
title_sort cmwrky6–1–cmwrky15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in chrysanthemum morifolium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419886/
https://www.ncbi.nlm.nih.gov/pubmed/37577400
http://dx.doi.org/10.1093/hr/uhad101
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