Cargando…
CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium
Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chr...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419886/ https://www.ncbi.nlm.nih.gov/pubmed/37577400 http://dx.doi.org/10.1093/hr/uhad101 |
_version_ | 1785088637204955136 |
---|---|
author | Miao, Weihao Xiao, Xiangyu Wang, Yuean Ge, Lijiao Yang, Yanrong Liu, Ye Liao, Yuan Guan, Zhiyong Chen, Sumei Fang, Weimin Chen, Fadi Zhao, Shuang |
author_facet | Miao, Weihao Xiao, Xiangyu Wang, Yuean Ge, Lijiao Yang, Yanrong Liu, Ye Liao, Yuan Guan, Zhiyong Chen, Sumei Fang, Weimin Chen, Fadi Zhao, Shuang |
author_sort | Miao, Weihao |
collection | PubMed |
description | Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chrysanthemum ‘Jinba’ and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that CmWRKY6–1 negatively regulated resistance to F. oxysporum and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression. Additionally, we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F. oxysporum via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F. oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system. |
format | Online Article Text |
id | pubmed-10419886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-104198862023-08-12 CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium Miao, Weihao Xiao, Xiangyu Wang, Yuean Ge, Lijiao Yang, Yanrong Liu, Ye Liao, Yuan Guan, Zhiyong Chen, Sumei Fang, Weimin Chen, Fadi Zhao, Shuang Hortic Res Article Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to Fusarium oxysporum infection remains largely unknown. In this study, we isolated CmWRKY6–1 from chrysanthemum ‘Jinba’ and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that CmWRKY6–1 negatively regulated resistance to F. oxysporum and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, CmWRKY6–1 bound to the W-box element on the CmWRKY15-like promoter and inhibited its expression. Additionally, we observed that CmWRKY15-like silencing in chrysanthemum reduced its resistance to F. oxysporum via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6–1–CmWRKY15-like cascade response to F. oxysporum infection in chrysanthemum and demonstrated that CmWRKY6–1 and CmWRKY15-like regulates the immune system. Oxford University Press 2023-05-10 /pmc/articles/PMC10419886/ /pubmed/37577400 http://dx.doi.org/10.1093/hr/uhad101 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Miao, Weihao Xiao, Xiangyu Wang, Yuean Ge, Lijiao Yang, Yanrong Liu, Ye Liao, Yuan Guan, Zhiyong Chen, Sumei Fang, Weimin Chen, Fadi Zhao, Shuang CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title | CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title_full | CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title_fullStr | CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title_full_unstemmed | CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title_short | CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium |
title_sort | cmwrky6–1–cmwrky15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in chrysanthemum morifolium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419886/ https://www.ncbi.nlm.nih.gov/pubmed/37577400 http://dx.doi.org/10.1093/hr/uhad101 |
work_keys_str_mv | AT miaoweihao cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT xiaoxiangyu cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT wangyuean cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT gelijiao cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT yangyanrong cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT liuye cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT liaoyuan cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT guanzhiyong cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT chensumei cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT fangweimin cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT chenfadi cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium AT zhaoshuang cmwrky61cmwrky15liketranscriptionalcascadenegativelyregulatestheresistancetofusariumoxysporuminfectioninchrysanthemummorifolium |