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Research on Cartilage 3D Printing Technology Based on SA-GA-HA
Cartilage damage is difficult to heal and poses a serious problem to human health as it can lead to osteoarthritis. In this work, we explore the application of biological 3D printing to manufacture new cartilage scaffolds to promote cartilage regeneration. The hydrogel made by mixing sodium alginate...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419889/ https://www.ncbi.nlm.nih.gov/pubmed/37570016 http://dx.doi.org/10.3390/ma16155312 |
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author | Chen, Yong Gong, Youping Shan, Lijun Tan, Chou Yong Al-Furjan, MS Ramesh, S. Chen, Huipeng Bian, Xiangjuan Chen, Yanda Liu, Yunfeng Zhou, Rougang |
author_facet | Chen, Yong Gong, Youping Shan, Lijun Tan, Chou Yong Al-Furjan, MS Ramesh, S. Chen, Huipeng Bian, Xiangjuan Chen, Yanda Liu, Yunfeng Zhou, Rougang |
author_sort | Chen, Yong |
collection | PubMed |
description | Cartilage damage is difficult to heal and poses a serious problem to human health as it can lead to osteoarthritis. In this work, we explore the application of biological 3D printing to manufacture new cartilage scaffolds to promote cartilage regeneration. The hydrogel made by mixing sodium alginate (SA) and gelatin (GA) has high biocompatibility, but its mechanical properties are poor. The addition of hydroxyapatite (HA) can enhance its mechanical properties. In this paper, the preparation scheme of the SA-GA-HA composite hydrogel cartilage scaffold was explored, the scaffolds prepared with different concentrations were compared, and better formulations were obtained for printing and testing. Mathematical modeling of the printing process of the bracket, simulation analysis of the printing process based on the mathematical model, and adjustment of actual printing parameters based on the results of the simulation were performed. The cartilage scaffold, which was printed using Bioplotter 3D printer, exhibited useful mechanical properties suitable for practical needs. In addition, ATDC-5 cells were seeded on the cartilage scaffolds and the cell survival rate was found to be higher after one week. The findings demonstrated that the fabricated chondrocyte scaffolds had better mechanical properties and biocompatibility, providing a new scaffold strategy for cartilage tissue regeneration. |
format | Online Article Text |
id | pubmed-10419889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104198892023-08-12 Research on Cartilage 3D Printing Technology Based on SA-GA-HA Chen, Yong Gong, Youping Shan, Lijun Tan, Chou Yong Al-Furjan, MS Ramesh, S. Chen, Huipeng Bian, Xiangjuan Chen, Yanda Liu, Yunfeng Zhou, Rougang Materials (Basel) Article Cartilage damage is difficult to heal and poses a serious problem to human health as it can lead to osteoarthritis. In this work, we explore the application of biological 3D printing to manufacture new cartilage scaffolds to promote cartilage regeneration. The hydrogel made by mixing sodium alginate (SA) and gelatin (GA) has high biocompatibility, but its mechanical properties are poor. The addition of hydroxyapatite (HA) can enhance its mechanical properties. In this paper, the preparation scheme of the SA-GA-HA composite hydrogel cartilage scaffold was explored, the scaffolds prepared with different concentrations were compared, and better formulations were obtained for printing and testing. Mathematical modeling of the printing process of the bracket, simulation analysis of the printing process based on the mathematical model, and adjustment of actual printing parameters based on the results of the simulation were performed. The cartilage scaffold, which was printed using Bioplotter 3D printer, exhibited useful mechanical properties suitable for practical needs. In addition, ATDC-5 cells were seeded on the cartilage scaffolds and the cell survival rate was found to be higher after one week. The findings demonstrated that the fabricated chondrocyte scaffolds had better mechanical properties and biocompatibility, providing a new scaffold strategy for cartilage tissue regeneration. MDPI 2023-07-28 /pmc/articles/PMC10419889/ /pubmed/37570016 http://dx.doi.org/10.3390/ma16155312 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yong Gong, Youping Shan, Lijun Tan, Chou Yong Al-Furjan, MS Ramesh, S. Chen, Huipeng Bian, Xiangjuan Chen, Yanda Liu, Yunfeng Zhou, Rougang Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title | Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title_full | Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title_fullStr | Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title_full_unstemmed | Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title_short | Research on Cartilage 3D Printing Technology Based on SA-GA-HA |
title_sort | research on cartilage 3d printing technology based on sa-ga-ha |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419889/ https://www.ncbi.nlm.nih.gov/pubmed/37570016 http://dx.doi.org/10.3390/ma16155312 |
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