Cargando…

Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation

Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been r...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wei, Gao, Lin, Zhao, Tianliang, Chen, Jiamei, Chen, Ting, Lin, Wenxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419918/
https://www.ncbi.nlm.nih.gov/pubmed/37569819
http://dx.doi.org/10.3390/ijms241512444
_version_ 1785088645731975168
author Wang, Wei
Gao, Lin
Zhao, Tianliang
Chen, Jiamei
Chen, Ting
Lin, Wenxiong
author_facet Wang, Wei
Gao, Lin
Zhao, Tianliang
Chen, Jiamei
Chen, Ting
Lin, Wenxiong
author_sort Wang, Wei
collection PubMed
description Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been reported. Here, we focused on identifying the transcription factors (TFs) involved in blue light-mediated hypocotyl growth. We analyzed the blue-light-mediated hypocotyl lengths of Arabidopsis TF–overexpressing lines and identified three NF–YC proteins, NF–YC7, NF–YC5, and NF–YC8 (NF–YCs being the short name), as the negative regulators in blue light-inhibited hypocotyl elongation. NF–YC–overexpressing lines developed longer hypocotyls than those of the wild type under blue light, while the deficient mutants nf–yc5nf–yc7 and nf–yc7nf–yc8 failed to exhibit hypocotyl elongation under blue light. NF–YCs physically interacted with CRY2 (cryptochrome 2) and PIF4/5 (phytochrome interacting factor 4 or 5), while the NF–YCs–PIF4/5 interactions were repressed by CRY2. Moreover, the overexpression of CRY2 or deficiency of PIF4/5 repressed NF–YC7–induced hypocotyl elongation under blue light. Further investigation revealed that NF–YC7 may increase CRY2 degradation and regulate PIF4/5 activities under blue light. Taken together, this study will provide new insight into the mechanism of how blue light inhibits hypocotyl elongation.
format Online
Article
Text
id pubmed-10419918
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104199182023-08-12 Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation Wang, Wei Gao, Lin Zhao, Tianliang Chen, Jiamei Chen, Ting Lin, Wenxiong Int J Mol Sci Article Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been reported. Here, we focused on identifying the transcription factors (TFs) involved in blue light-mediated hypocotyl growth. We analyzed the blue-light-mediated hypocotyl lengths of Arabidopsis TF–overexpressing lines and identified three NF–YC proteins, NF–YC7, NF–YC5, and NF–YC8 (NF–YCs being the short name), as the negative regulators in blue light-inhibited hypocotyl elongation. NF–YC–overexpressing lines developed longer hypocotyls than those of the wild type under blue light, while the deficient mutants nf–yc5nf–yc7 and nf–yc7nf–yc8 failed to exhibit hypocotyl elongation under blue light. NF–YCs physically interacted with CRY2 (cryptochrome 2) and PIF4/5 (phytochrome interacting factor 4 or 5), while the NF–YCs–PIF4/5 interactions were repressed by CRY2. Moreover, the overexpression of CRY2 or deficiency of PIF4/5 repressed NF–YC7–induced hypocotyl elongation under blue light. Further investigation revealed that NF–YC7 may increase CRY2 degradation and regulate PIF4/5 activities under blue light. Taken together, this study will provide new insight into the mechanism of how blue light inhibits hypocotyl elongation. MDPI 2023-08-04 /pmc/articles/PMC10419918/ /pubmed/37569819 http://dx.doi.org/10.3390/ijms241512444 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Wei
Gao, Lin
Zhao, Tianliang
Chen, Jiamei
Chen, Ting
Lin, Wenxiong
Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title_full Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title_fullStr Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title_full_unstemmed Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title_short Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
title_sort arabidopsis nf–yc7 interacts with cry2 and pif4/5 to repress blue light-inhibited hypocotyl elongation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419918/
https://www.ncbi.nlm.nih.gov/pubmed/37569819
http://dx.doi.org/10.3390/ijms241512444
work_keys_str_mv AT wangwei arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation
AT gaolin arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation
AT zhaotianliang arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation
AT chenjiamei arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation
AT chenting arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation
AT linwenxiong arabidopsisnfyc7interactswithcry2andpif45torepressbluelightinhibitedhypocotylelongation