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Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation
Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419918/ https://www.ncbi.nlm.nih.gov/pubmed/37569819 http://dx.doi.org/10.3390/ijms241512444 |
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author | Wang, Wei Gao, Lin Zhao, Tianliang Chen, Jiamei Chen, Ting Lin, Wenxiong |
author_facet | Wang, Wei Gao, Lin Zhao, Tianliang Chen, Jiamei Chen, Ting Lin, Wenxiong |
author_sort | Wang, Wei |
collection | PubMed |
description | Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been reported. Here, we focused on identifying the transcription factors (TFs) involved in blue light-mediated hypocotyl growth. We analyzed the blue-light-mediated hypocotyl lengths of Arabidopsis TF–overexpressing lines and identified three NF–YC proteins, NF–YC7, NF–YC5, and NF–YC8 (NF–YCs being the short name), as the negative regulators in blue light-inhibited hypocotyl elongation. NF–YC–overexpressing lines developed longer hypocotyls than those of the wild type under blue light, while the deficient mutants nf–yc5nf–yc7 and nf–yc7nf–yc8 failed to exhibit hypocotyl elongation under blue light. NF–YCs physically interacted with CRY2 (cryptochrome 2) and PIF4/5 (phytochrome interacting factor 4 or 5), while the NF–YCs–PIF4/5 interactions were repressed by CRY2. Moreover, the overexpression of CRY2 or deficiency of PIF4/5 repressed NF–YC7–induced hypocotyl elongation under blue light. Further investigation revealed that NF–YC7 may increase CRY2 degradation and regulate PIF4/5 activities under blue light. Taken together, this study will provide new insight into the mechanism of how blue light inhibits hypocotyl elongation. |
format | Online Article Text |
id | pubmed-10419918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104199182023-08-12 Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation Wang, Wei Gao, Lin Zhao, Tianliang Chen, Jiamei Chen, Ting Lin, Wenxiong Int J Mol Sci Article Light is an important environmental factor. Plants adapt to their light environment by developing the optimal phenotypes. Light-mediated hypocotyl growth is an ideal phenotype for studying how plants respond to light. Thus far, many signaling components in light-mediated hypocotyl growth have been reported. Here, we focused on identifying the transcription factors (TFs) involved in blue light-mediated hypocotyl growth. We analyzed the blue-light-mediated hypocotyl lengths of Arabidopsis TF–overexpressing lines and identified three NF–YC proteins, NF–YC7, NF–YC5, and NF–YC8 (NF–YCs being the short name), as the negative regulators in blue light-inhibited hypocotyl elongation. NF–YC–overexpressing lines developed longer hypocotyls than those of the wild type under blue light, while the deficient mutants nf–yc5nf–yc7 and nf–yc7nf–yc8 failed to exhibit hypocotyl elongation under blue light. NF–YCs physically interacted with CRY2 (cryptochrome 2) and PIF4/5 (phytochrome interacting factor 4 or 5), while the NF–YCs–PIF4/5 interactions were repressed by CRY2. Moreover, the overexpression of CRY2 or deficiency of PIF4/5 repressed NF–YC7–induced hypocotyl elongation under blue light. Further investigation revealed that NF–YC7 may increase CRY2 degradation and regulate PIF4/5 activities under blue light. Taken together, this study will provide new insight into the mechanism of how blue light inhibits hypocotyl elongation. MDPI 2023-08-04 /pmc/articles/PMC10419918/ /pubmed/37569819 http://dx.doi.org/10.3390/ijms241512444 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Wei Gao, Lin Zhao, Tianliang Chen, Jiamei Chen, Ting Lin, Wenxiong Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title | Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title_full | Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title_fullStr | Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title_full_unstemmed | Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title_short | Arabidopsis NF–YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation |
title_sort | arabidopsis nf–yc7 interacts with cry2 and pif4/5 to repress blue light-inhibited hypocotyl elongation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419918/ https://www.ncbi.nlm.nih.gov/pubmed/37569819 http://dx.doi.org/10.3390/ijms241512444 |
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