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Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model

The exact mechanism of desmoplastic stromal reaction (DSR) formation is still unclear. The interaction between cancer cells and cancer-associated fibroblasts (CAFs) has an important role in tumor progression, while stromal changes are a poor prognostic factor in pleural mesothelioma (PM). We aimed t...

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Autores principales: Borchert, Sabrina, Mathilakathu, Alexander, Nath, Alina, Wessolly, Michael, Mairinger, Elena, Kreidt, Daniel, Steinborn, Julia, Walter, Robert F. H., Christoph, Daniel C., Kollmeier, Jens, Wohlschlaeger, Jeremias, Mairinger, Thomas, Brcic, Luka, Mairinger, Fabian D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419996/
https://www.ncbi.nlm.nih.gov/pubmed/37569808
http://dx.doi.org/10.3390/ijms241512426
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author Borchert, Sabrina
Mathilakathu, Alexander
Nath, Alina
Wessolly, Michael
Mairinger, Elena
Kreidt, Daniel
Steinborn, Julia
Walter, Robert F. H.
Christoph, Daniel C.
Kollmeier, Jens
Wohlschlaeger, Jeremias
Mairinger, Thomas
Brcic, Luka
Mairinger, Fabian D.
author_facet Borchert, Sabrina
Mathilakathu, Alexander
Nath, Alina
Wessolly, Michael
Mairinger, Elena
Kreidt, Daniel
Steinborn, Julia
Walter, Robert F. H.
Christoph, Daniel C.
Kollmeier, Jens
Wohlschlaeger, Jeremias
Mairinger, Thomas
Brcic, Luka
Mairinger, Fabian D.
author_sort Borchert, Sabrina
collection PubMed
description The exact mechanism of desmoplastic stromal reaction (DSR) formation is still unclear. The interaction between cancer cells and cancer-associated fibroblasts (CAFs) has an important role in tumor progression, while stromal changes are a poor prognostic factor in pleural mesothelioma (PM). We aimed to assess the impact of CAFs paracrine signaling within the tumor microenvironment and the DSR presence on survival, in a cohort of 77 PM patients. DSR formation was evaluated morphologically and by immunohistochemistry for Fibroblast activation protein alpha (FAP). Digital gene expression was analyzed using a custom-designed CodeSet (NanoString). Decision-tree-based analysis using the “conditional inference tree” (CIT) machine learning algorithm was performed on the obtained results. A significant association between FAP gene expression levels and the appearance of DSR was found (p = 0.025). DSR-high samples demonstrated a statistically significant prolonged median survival time. The elevated expression of MYT1, KDR, PIK3R1, PIK3R4, and SOS1 was associated with shortened OS, whereas the upregulation of VEGFC, FAP, and CDK4 was associated with prolonged OS. CIT revealed a three-tier system based on FAP, NF1, and RPTOR expressions. We could outline the prognostic value of CAFs-induced PI3K signaling pathway activation together with FAP-dependent CDK4 mediated cell cycle progression in PM, where prognostic and predictive biomarkers are urgently needed to introduce new therapeutic strategies.
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spelling pubmed-104199962023-08-12 Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model Borchert, Sabrina Mathilakathu, Alexander Nath, Alina Wessolly, Michael Mairinger, Elena Kreidt, Daniel Steinborn, Julia Walter, Robert F. H. Christoph, Daniel C. Kollmeier, Jens Wohlschlaeger, Jeremias Mairinger, Thomas Brcic, Luka Mairinger, Fabian D. Int J Mol Sci Article The exact mechanism of desmoplastic stromal reaction (DSR) formation is still unclear. The interaction between cancer cells and cancer-associated fibroblasts (CAFs) has an important role in tumor progression, while stromal changes are a poor prognostic factor in pleural mesothelioma (PM). We aimed to assess the impact of CAFs paracrine signaling within the tumor microenvironment and the DSR presence on survival, in a cohort of 77 PM patients. DSR formation was evaluated morphologically and by immunohistochemistry for Fibroblast activation protein alpha (FAP). Digital gene expression was analyzed using a custom-designed CodeSet (NanoString). Decision-tree-based analysis using the “conditional inference tree” (CIT) machine learning algorithm was performed on the obtained results. A significant association between FAP gene expression levels and the appearance of DSR was found (p = 0.025). DSR-high samples demonstrated a statistically significant prolonged median survival time. The elevated expression of MYT1, KDR, PIK3R1, PIK3R4, and SOS1 was associated with shortened OS, whereas the upregulation of VEGFC, FAP, and CDK4 was associated with prolonged OS. CIT revealed a three-tier system based on FAP, NF1, and RPTOR expressions. We could outline the prognostic value of CAFs-induced PI3K signaling pathway activation together with FAP-dependent CDK4 mediated cell cycle progression in PM, where prognostic and predictive biomarkers are urgently needed to introduce new therapeutic strategies. MDPI 2023-08-04 /pmc/articles/PMC10419996/ /pubmed/37569808 http://dx.doi.org/10.3390/ijms241512426 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borchert, Sabrina
Mathilakathu, Alexander
Nath, Alina
Wessolly, Michael
Mairinger, Elena
Kreidt, Daniel
Steinborn, Julia
Walter, Robert F. H.
Christoph, Daniel C.
Kollmeier, Jens
Wohlschlaeger, Jeremias
Mairinger, Thomas
Brcic, Luka
Mairinger, Fabian D.
Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title_full Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title_fullStr Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title_full_unstemmed Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title_short Cancer-Associated Fibroblasts Influence Survival in Pleural Mesothelioma: Digital Gene Expression Analysis and Supervised Machine Learning Model
title_sort cancer-associated fibroblasts influence survival in pleural mesothelioma: digital gene expression analysis and supervised machine learning model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419996/
https://www.ncbi.nlm.nih.gov/pubmed/37569808
http://dx.doi.org/10.3390/ijms241512426
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