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Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken

The role of gga-miR-31 in chicken germ cell differentiation and spermatogenesis is of significant importance. The transcriptional properties of gga-miR-31 are crucial in establishing the foundation for the formation of chicken spermatogonia stem cells and spermatogenesis. In this study, a series of...

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Autores principales: Wang, Yingjie, Kong, Ruihong, Xie, Ke, Hu, Cai, Zhao, Zongyi, Wu, Yuhui, Zuo, Qisheng, Li, Bichun, Zhang, Yani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420004/
https://www.ncbi.nlm.nih.gov/pubmed/37569891
http://dx.doi.org/10.3390/ijms241512516
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author Wang, Yingjie
Kong, Ruihong
Xie, Ke
Hu, Cai
Zhao, Zongyi
Wu, Yuhui
Zuo, Qisheng
Li, Bichun
Zhang, Yani
author_facet Wang, Yingjie
Kong, Ruihong
Xie, Ke
Hu, Cai
Zhao, Zongyi
Wu, Yuhui
Zuo, Qisheng
Li, Bichun
Zhang, Yani
author_sort Wang, Yingjie
collection PubMed
description The role of gga-miR-31 in chicken germ cell differentiation and spermatogenesis is of significant importance. The transcriptional properties of gga-miR-31 are crucial in establishing the foundation for the formation of chicken spermatogonia stem cells and spermatogenesis. In this study, a series of recombinant vectors including varying lengths of the gga-miR-31 promoter were predicted and constructed. Through the utilization of the dual luciferase reporting system, the upstream −2180~0 bp region of gga-miR-31 was identified as its promoter region. Furthermore, it was predicted and confirmed that the activity of the gga-miR-31 promoter is increased by retinoic acid (RA). The binding of RA to the gga-miR-31 and Stra8 promoter regions was found to be competitive. Through the deletion of C-jun binding sites and the manipulation of C-jun expression levels, it was determined that C-jun inhibits the activity of the gga-miR-31 promoter. Furthermore, the combined treatment of C-jun and RA demonstrated that the positive regulatory effect of RA on the gga-miR-31 promoter is attenuated in the presence of high levels of C-jun. Overall, this study establishes a foundation for further investigation into the regulatory mechanisms of gga-miR-31 action, and provides a new avenue for inducing chicken embryonic stem cells (ESC) to differentiate into spermatogonial stem cells (SSC), and sperm formation.
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spelling pubmed-104200042023-08-12 Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken Wang, Yingjie Kong, Ruihong Xie, Ke Hu, Cai Zhao, Zongyi Wu, Yuhui Zuo, Qisheng Li, Bichun Zhang, Yani Int J Mol Sci Article The role of gga-miR-31 in chicken germ cell differentiation and spermatogenesis is of significant importance. The transcriptional properties of gga-miR-31 are crucial in establishing the foundation for the formation of chicken spermatogonia stem cells and spermatogenesis. In this study, a series of recombinant vectors including varying lengths of the gga-miR-31 promoter were predicted and constructed. Through the utilization of the dual luciferase reporting system, the upstream −2180~0 bp region of gga-miR-31 was identified as its promoter region. Furthermore, it was predicted and confirmed that the activity of the gga-miR-31 promoter is increased by retinoic acid (RA). The binding of RA to the gga-miR-31 and Stra8 promoter regions was found to be competitive. Through the deletion of C-jun binding sites and the manipulation of C-jun expression levels, it was determined that C-jun inhibits the activity of the gga-miR-31 promoter. Furthermore, the combined treatment of C-jun and RA demonstrated that the positive regulatory effect of RA on the gga-miR-31 promoter is attenuated in the presence of high levels of C-jun. Overall, this study establishes a foundation for further investigation into the regulatory mechanisms of gga-miR-31 action, and provides a new avenue for inducing chicken embryonic stem cells (ESC) to differentiate into spermatogonial stem cells (SSC), and sperm formation. MDPI 2023-08-07 /pmc/articles/PMC10420004/ /pubmed/37569891 http://dx.doi.org/10.3390/ijms241512516 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yingjie
Kong, Ruihong
Xie, Ke
Hu, Cai
Zhao, Zongyi
Wu, Yuhui
Zuo, Qisheng
Li, Bichun
Zhang, Yani
Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title_full Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title_fullStr Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title_full_unstemmed Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title_short Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
title_sort analysis of the promoter regions of gga-mir-31 and its regulation by ra and c-jun in chicken
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420004/
https://www.ncbi.nlm.nih.gov/pubmed/37569891
http://dx.doi.org/10.3390/ijms241512516
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