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Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile

Advanced age has been reported to negatively affect sperm parameters and spermatozoa DNA integrity. A decline in sperm criteria was also associated with altered epigenetic marks such as DNA methylation with a potential downstream impact on in vitro fertilization success and clinical outcomes. The ai...

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Autores principales: Lahimer, Marwa, Montjean, Debbie, Cabry, Rosalie, Capelle, Severine, Lefranc, Elodie, Bach, Véronique, Ajina, Mounir, Ben Ali, Habib, Khorsi-Cauet, Hafida, Benkhalifa, Moncef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420110/
https://www.ncbi.nlm.nih.gov/pubmed/37568329
http://dx.doi.org/10.3390/jcm12154928
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author Lahimer, Marwa
Montjean, Debbie
Cabry, Rosalie
Capelle, Severine
Lefranc, Elodie
Bach, Véronique
Ajina, Mounir
Ben Ali, Habib
Khorsi-Cauet, Hafida
Benkhalifa, Moncef
author_facet Lahimer, Marwa
Montjean, Debbie
Cabry, Rosalie
Capelle, Severine
Lefranc, Elodie
Bach, Véronique
Ajina, Mounir
Ben Ali, Habib
Khorsi-Cauet, Hafida
Benkhalifa, Moncef
author_sort Lahimer, Marwa
collection PubMed
description Advanced age has been reported to negatively affect sperm parameters and spermatozoa DNA integrity. A decline in sperm criteria was also associated with altered epigenetic marks such as DNA methylation with a potential downstream impact on in vitro fertilization success and clinical outcomes. The aim of the present retrospective study was to clarify the association between advanced paternal age (APA) and sperm parameters, DNA integrity and DNA methylation profile. A total of 671 patients consulting for infertility underwent sperm analysis, sperm DNA integrity assessment and methylation level measurement. The principal finding was that individuals over 40 years of age exhibit a significant increase in DNA fragmentation levels compared to the younger group (15% versus 9%, respectively, p = 0.04). However, there was no significant difference in DNA decondensation and sperm parameters in association with APA. In addition, a drop in the global methylation level was also found in men over 40 years (6% in the young group versus 2% in the old group, p = 0.03). As a conclusion, men over 40 years are at higher risk of elevated sperm DNA fragmentation and lower methylation level. Based on these observations, it is recommended that the assessment of sperm DNA fragmentation should be taken into consideration particularly after the age of 40. Our findings support the idea that paternal age is a crucial factor that should not be neglected during fertility evaluation and treatment since it is associated with epigenetics changes in sperm. Although the underlying mechanism remains to be clarified, we believe that environmental and professional exposure factors are likely involved in the process.
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spelling pubmed-104201102023-08-12 Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile Lahimer, Marwa Montjean, Debbie Cabry, Rosalie Capelle, Severine Lefranc, Elodie Bach, Véronique Ajina, Mounir Ben Ali, Habib Khorsi-Cauet, Hafida Benkhalifa, Moncef J Clin Med Article Advanced age has been reported to negatively affect sperm parameters and spermatozoa DNA integrity. A decline in sperm criteria was also associated with altered epigenetic marks such as DNA methylation with a potential downstream impact on in vitro fertilization success and clinical outcomes. The aim of the present retrospective study was to clarify the association between advanced paternal age (APA) and sperm parameters, DNA integrity and DNA methylation profile. A total of 671 patients consulting for infertility underwent sperm analysis, sperm DNA integrity assessment and methylation level measurement. The principal finding was that individuals over 40 years of age exhibit a significant increase in DNA fragmentation levels compared to the younger group (15% versus 9%, respectively, p = 0.04). However, there was no significant difference in DNA decondensation and sperm parameters in association with APA. In addition, a drop in the global methylation level was also found in men over 40 years (6% in the young group versus 2% in the old group, p = 0.03). As a conclusion, men over 40 years are at higher risk of elevated sperm DNA fragmentation and lower methylation level. Based on these observations, it is recommended that the assessment of sperm DNA fragmentation should be taken into consideration particularly after the age of 40. Our findings support the idea that paternal age is a crucial factor that should not be neglected during fertility evaluation and treatment since it is associated with epigenetics changes in sperm. Although the underlying mechanism remains to be clarified, we believe that environmental and professional exposure factors are likely involved in the process. MDPI 2023-07-27 /pmc/articles/PMC10420110/ /pubmed/37568329 http://dx.doi.org/10.3390/jcm12154928 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lahimer, Marwa
Montjean, Debbie
Cabry, Rosalie
Capelle, Severine
Lefranc, Elodie
Bach, Véronique
Ajina, Mounir
Ben Ali, Habib
Khorsi-Cauet, Hafida
Benkhalifa, Moncef
Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title_full Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title_fullStr Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title_full_unstemmed Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title_short Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile
title_sort paternal age matters: association with sperm criteria’s- spermatozoa dna integrity and methylation profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420110/
https://www.ncbi.nlm.nih.gov/pubmed/37568329
http://dx.doi.org/10.3390/jcm12154928
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