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Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds

Arabinoxylan has prebiotic properties, as it is able to resist digestion in the small intestine and undergoes fermentation in the large intestine. In this work, arabinoxylan was extracted from corn fiber using an alkaline solution and further purified with membrane processing. It was found that the...

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Autores principales: Weng, Verónica, Cardeira, Martim, Bento-Silva, Andreia, Serra, Ana Teresa, Brazinha, Carla, Bronze, Maria Rosário
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420191/
https://www.ncbi.nlm.nih.gov/pubmed/37570593
http://dx.doi.org/10.3390/molecules28155621
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author Weng, Verónica
Cardeira, Martim
Bento-Silva, Andreia
Serra, Ana Teresa
Brazinha, Carla
Bronze, Maria Rosário
author_facet Weng, Verónica
Cardeira, Martim
Bento-Silva, Andreia
Serra, Ana Teresa
Brazinha, Carla
Bronze, Maria Rosário
author_sort Weng, Verónica
collection PubMed
description Arabinoxylan has prebiotic properties, as it is able to resist digestion in the small intestine and undergoes fermentation in the large intestine. In this work, arabinoxylan was extracted from corn fiber using an alkaline solution and further purified with membrane processing. It was found that the extracts were mainly composed of xylose (50–52%), arabinose (37–39%), galactose (9%) and glucose (1–4%), with an A/X ratio of 0.72–0.77. All the extracts were composed of phenolic compounds, including ferulic acid derivatives such as dimers, trimers and tetramers. The purified extract had a lower concentration of ferulic and p-coumaric acid (0.004 and 0.02 mg/mg(dry_weight), respectively) when compared to raw extract (19.30 and 2.74 mg/mg(dry_weight), respectively). The same effect was observed for the antioxidant activity, with purified extracts having a lower value (0.17 ± 0.02 µmol TEAC/mg) when compared to the raw extract (2.20 ± 0.35 µmol TEAC/mg). The purified extract showed a greater antiproliferative effect against the HT29 cell line with EC(50) = 0.12 ± 0.02 mg/mL when compared to the raw extract (EC(50) = 5.60 ± 1.6 mg/mL). Both raw and purified extracts did not show any cytotoxicity to the Caco-2 cell line in the maximum concentration tested (10 mg/mL).
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spelling pubmed-104201912023-08-12 Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds Weng, Verónica Cardeira, Martim Bento-Silva, Andreia Serra, Ana Teresa Brazinha, Carla Bronze, Maria Rosário Molecules Article Arabinoxylan has prebiotic properties, as it is able to resist digestion in the small intestine and undergoes fermentation in the large intestine. In this work, arabinoxylan was extracted from corn fiber using an alkaline solution and further purified with membrane processing. It was found that the extracts were mainly composed of xylose (50–52%), arabinose (37–39%), galactose (9%) and glucose (1–4%), with an A/X ratio of 0.72–0.77. All the extracts were composed of phenolic compounds, including ferulic acid derivatives such as dimers, trimers and tetramers. The purified extract had a lower concentration of ferulic and p-coumaric acid (0.004 and 0.02 mg/mg(dry_weight), respectively) when compared to raw extract (19.30 and 2.74 mg/mg(dry_weight), respectively). The same effect was observed for the antioxidant activity, with purified extracts having a lower value (0.17 ± 0.02 µmol TEAC/mg) when compared to the raw extract (2.20 ± 0.35 µmol TEAC/mg). The purified extract showed a greater antiproliferative effect against the HT29 cell line with EC(50) = 0.12 ± 0.02 mg/mL when compared to the raw extract (EC(50) = 5.60 ± 1.6 mg/mL). Both raw and purified extracts did not show any cytotoxicity to the Caco-2 cell line in the maximum concentration tested (10 mg/mL). MDPI 2023-07-25 /pmc/articles/PMC10420191/ /pubmed/37570593 http://dx.doi.org/10.3390/molecules28155621 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weng, Verónica
Cardeira, Martim
Bento-Silva, Andreia
Serra, Ana Teresa
Brazinha, Carla
Bronze, Maria Rosário
Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title_full Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title_fullStr Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title_full_unstemmed Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title_short Arabinoxylan from Corn Fiber Obtained through Alkaline Extraction and Membrane Purification: Relating Bioactivities with the Phenolic Compounds
title_sort arabinoxylan from corn fiber obtained through alkaline extraction and membrane purification: relating bioactivities with the phenolic compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420191/
https://www.ncbi.nlm.nih.gov/pubmed/37570593
http://dx.doi.org/10.3390/molecules28155621
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