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Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420707/ https://www.ncbi.nlm.nih.gov/pubmed/37576762 http://dx.doi.org/10.14202/vetworld.2023.1001-1007 |
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author | Salem, Gamal A. Abdelaziz, El-Sayed A. Kamel, Mohammed A. Rhouma, Nasreddin R. Ali, Reem I. |
author_facet | Salem, Gamal A. Abdelaziz, El-Sayed A. Kamel, Mohammed A. Rhouma, Nasreddin R. Ali, Reem I. |
author_sort | Salem, Gamal A. |
collection | PubMed |
description | BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt. MATERIALS AND METHODS: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the β-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction. RESULTS: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively. CONCLUSION: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem. |
format | Online Article Text |
id | pubmed-10420707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-104207072023-08-12 Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt Salem, Gamal A. Abdelaziz, El-Sayed A. Kamel, Mohammed A. Rhouma, Nasreddin R. Ali, Reem I. Vet World Research Article BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt. MATERIALS AND METHODS: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the β-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction. RESULTS: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively. CONCLUSION: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem. Veterinary World 2023-05 2023-05-13 /pmc/articles/PMC10420707/ /pubmed/37576762 http://dx.doi.org/10.14202/vetworld.2023.1001-1007 Text en Copyright: © Salem, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Salem, Gamal A. Abdelaziz, El-Sayed A. Kamel, Mohammed A. Rhouma, Nasreddin R. Ali, Reem I. Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title | Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title_full | Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title_fullStr | Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title_full_unstemmed | Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title_short | Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt |
title_sort | prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing escherichia coli from chicken farms in egypt |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420707/ https://www.ncbi.nlm.nih.gov/pubmed/37576762 http://dx.doi.org/10.14202/vetworld.2023.1001-1007 |
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