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Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt

BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts...

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Autores principales: Salem, Gamal A., Abdelaziz, El-Sayed A., Kamel, Mohammed A., Rhouma, Nasreddin R., Ali, Reem I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420707/
https://www.ncbi.nlm.nih.gov/pubmed/37576762
http://dx.doi.org/10.14202/vetworld.2023.1001-1007
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author Salem, Gamal A.
Abdelaziz, El-Sayed A.
Kamel, Mohammed A.
Rhouma, Nasreddin R.
Ali, Reem I.
author_facet Salem, Gamal A.
Abdelaziz, El-Sayed A.
Kamel, Mohammed A.
Rhouma, Nasreddin R.
Ali, Reem I.
author_sort Salem, Gamal A.
collection PubMed
description BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt. MATERIALS AND METHODS: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the β-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction. RESULTS: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively. CONCLUSION: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem.
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spelling pubmed-104207072023-08-12 Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt Salem, Gamal A. Abdelaziz, El-Sayed A. Kamel, Mohammed A. Rhouma, Nasreddin R. Ali, Reem I. Vet World Research Article BACKGROUND AND AIM: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt. MATERIALS AND METHODS: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the β-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction. RESULTS: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively. CONCLUSION: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem. Veterinary World 2023-05 2023-05-13 /pmc/articles/PMC10420707/ /pubmed/37576762 http://dx.doi.org/10.14202/vetworld.2023.1001-1007 Text en Copyright: © Salem, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Salem, Gamal A.
Abdelaziz, El-Sayed A.
Kamel, Mohammed A.
Rhouma, Nasreddin R.
Ali, Reem I.
Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title_full Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title_fullStr Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title_full_unstemmed Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title_short Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt
title_sort prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing escherichia coli from chicken farms in egypt
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420707/
https://www.ncbi.nlm.nih.gov/pubmed/37576762
http://dx.doi.org/10.14202/vetworld.2023.1001-1007
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