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Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota
The beneficial effect of probiotics on host health is impaired due to the substantial loss of survivability during gastric transit caused by small intestinal enzymes and bile acids. Encapsulation helps to preserve the probiotics species from severe environmental factors. Lactobacillus paracasei, hig...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420788/ https://www.ncbi.nlm.nih.gov/pubmed/37576064 http://dx.doi.org/10.1002/fsn3.3414 |
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author | Gyawali, Ishwari Zhou, Guilian Xu, Guli Li, Genghui Wang, Yujun Zeng, Yuxian Li, Jincheng Zhou, Jingjing Zhu, Canjun Shu, Gang Jiang, Qingyan |
author_facet | Gyawali, Ishwari Zhou, Guilian Xu, Guli Li, Genghui Wang, Yujun Zeng, Yuxian Li, Jincheng Zhou, Jingjing Zhu, Canjun Shu, Gang Jiang, Qingyan |
author_sort | Gyawali, Ishwari |
collection | PubMed |
description | The beneficial effect of probiotics on host health is impaired due to the substantial loss of survivability during gastric transit caused by small intestinal enzymes and bile acids. Encapsulation helps to preserve the probiotics species from severe environmental factors. Lactobacillus paracasei, highly sensitive probiotic species to gastric acid, was encapsulated with polyacrylate resin. C57BL/6 male mice were equally divided into three groups; control group was fed with basal diet without any additives, the un‐encapsulated group was fed with 0.1% of a mixture of encapsulating material and L. paracasei, and encapsulated group was fed with 0.1% encapsulated L. paracasei (microcapsule) for 4 weeks. The result showed elevated fecal moisture percentage in the encapsulated group, but not in the un‐encapsulated group. Further study showed that the ratio of villus height to crypt depth in the small intestine was significantly higher compared to un‐encapsulated and the control group. Microencapsulated probiotics also remarkably increased intestinal mucin and secretory immunoglobulin A (sIgA) concentration, intestinal MUC‐2, and tight junction protein mRNA expression levels improving the intestinal barrier function of mice. In addition, microcapsules also reduced proinflammatory factor mRNA expression, while considerably increasing anti‐inflammatory factor mRNA expression. Microbiota metabolites, fecal LPS (Lipopolysaccharide) were downregulated, and acetate and lactate were upraised compared to control. Furthermore, glutathione peroxidase (GSH‐Px) and TAOC levels were increased and Malondialdehyde (MDA) was decreased improving antioxidant capacity. Microflora and bioinformatic predictive analysis of feces showed that encapsulated probiotics remarkably increased Lactobacillus proportions. Mice's intestinal health can thus be improved by using microencapsulated probiotics. |
format | Online Article Text |
id | pubmed-10420788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104207882023-08-12 Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota Gyawali, Ishwari Zhou, Guilian Xu, Guli Li, Genghui Wang, Yujun Zeng, Yuxian Li, Jincheng Zhou, Jingjing Zhu, Canjun Shu, Gang Jiang, Qingyan Food Sci Nutr Original Articles The beneficial effect of probiotics on host health is impaired due to the substantial loss of survivability during gastric transit caused by small intestinal enzymes and bile acids. Encapsulation helps to preserve the probiotics species from severe environmental factors. Lactobacillus paracasei, highly sensitive probiotic species to gastric acid, was encapsulated with polyacrylate resin. C57BL/6 male mice were equally divided into three groups; control group was fed with basal diet without any additives, the un‐encapsulated group was fed with 0.1% of a mixture of encapsulating material and L. paracasei, and encapsulated group was fed with 0.1% encapsulated L. paracasei (microcapsule) for 4 weeks. The result showed elevated fecal moisture percentage in the encapsulated group, but not in the un‐encapsulated group. Further study showed that the ratio of villus height to crypt depth in the small intestine was significantly higher compared to un‐encapsulated and the control group. Microencapsulated probiotics also remarkably increased intestinal mucin and secretory immunoglobulin A (sIgA) concentration, intestinal MUC‐2, and tight junction protein mRNA expression levels improving the intestinal barrier function of mice. In addition, microcapsules also reduced proinflammatory factor mRNA expression, while considerably increasing anti‐inflammatory factor mRNA expression. Microbiota metabolites, fecal LPS (Lipopolysaccharide) were downregulated, and acetate and lactate were upraised compared to control. Furthermore, glutathione peroxidase (GSH‐Px) and TAOC levels were increased and Malondialdehyde (MDA) was decreased improving antioxidant capacity. Microflora and bioinformatic predictive analysis of feces showed that encapsulated probiotics remarkably increased Lactobacillus proportions. Mice's intestinal health can thus be improved by using microencapsulated probiotics. John Wiley and Sons Inc. 2023-05-10 /pmc/articles/PMC10420788/ /pubmed/37576064 http://dx.doi.org/10.1002/fsn3.3414 Text en © 2023 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Gyawali, Ishwari Zhou, Guilian Xu, Guli Li, Genghui Wang, Yujun Zeng, Yuxian Li, Jincheng Zhou, Jingjing Zhu, Canjun Shu, Gang Jiang, Qingyan Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title | Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title_full | Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title_fullStr | Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title_full_unstemmed | Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title_short | Supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
title_sort | supplementation of microencapsulated probiotics modulates gut health and intestinal microbiota |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420788/ https://www.ncbi.nlm.nih.gov/pubmed/37576064 http://dx.doi.org/10.1002/fsn3.3414 |
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