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The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies

Prostate cancer is a common cancer in men in older age groups. The WHO forecasts an increase in the incidence of prostate cancer in the coming years. Patients may not respond to treatment, and may not tolerate the side effects of chemotherapy. Compounds of natural origin have long been used in the p...

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Autores principales: Woźniak, Przemysław, Kleczka, Anna, Jasik, Krzysztof, Kabała-Dzik, Agata, Dzik, Radosław, Stojko, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420981/
https://www.ncbi.nlm.nih.gov/pubmed/37570691
http://dx.doi.org/10.3390/molecules28155719
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author Woźniak, Przemysław
Kleczka, Anna
Jasik, Krzysztof
Kabała-Dzik, Agata
Dzik, Radosław
Stojko, Jerzy
author_facet Woźniak, Przemysław
Kleczka, Anna
Jasik, Krzysztof
Kabała-Dzik, Agata
Dzik, Radosław
Stojko, Jerzy
author_sort Woźniak, Przemysław
collection PubMed
description Prostate cancer is a common cancer in men in older age groups. The WHO forecasts an increase in the incidence of prostate cancer in the coming years. Patients may not respond to treatment, and may not tolerate the side effects of chemotherapy. Compounds of natural origin have long been used in the prevention and treatment of cancer. Flavonoids obtained from natural products, e.g., propolis, are compounds with proven antibacterial and antiviral efficacy which modulate the immune response and may be useful as adjuvants in chemotherapy. The main aim of the present study was to evaluate the cytotoxic and pro-apoptotic properties of selected flavonoids on prostate cancer cells of the LNCaP line. The compounds used in this study were CAPE, curcumin (CUR), and quercetin (QUE). Mitochondrial and lysosome metabolism was assessed by the XTT-NR-SRB triple assay as well as by the fluorescent staining techniques. Staining for reactive oxygen species was performed as well. The experiment showed that each of the tested compounds has a cytotoxic effect on the LNCaP cell line. Different types of cell death were induced by the tested compounds. Apoptosis was induced by quercetin, while autophagy-specific changes were observed after using CAPE. Compounds obtained from other bee products have antiproliferative and cytotoxic activity against LNCaP prostate cancer cells.
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spelling pubmed-104209812023-08-12 The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies Woźniak, Przemysław Kleczka, Anna Jasik, Krzysztof Kabała-Dzik, Agata Dzik, Radosław Stojko, Jerzy Molecules Article Prostate cancer is a common cancer in men in older age groups. The WHO forecasts an increase in the incidence of prostate cancer in the coming years. Patients may not respond to treatment, and may not tolerate the side effects of chemotherapy. Compounds of natural origin have long been used in the prevention and treatment of cancer. Flavonoids obtained from natural products, e.g., propolis, are compounds with proven antibacterial and antiviral efficacy which modulate the immune response and may be useful as adjuvants in chemotherapy. The main aim of the present study was to evaluate the cytotoxic and pro-apoptotic properties of selected flavonoids on prostate cancer cells of the LNCaP line. The compounds used in this study were CAPE, curcumin (CUR), and quercetin (QUE). Mitochondrial and lysosome metabolism was assessed by the XTT-NR-SRB triple assay as well as by the fluorescent staining techniques. Staining for reactive oxygen species was performed as well. The experiment showed that each of the tested compounds has a cytotoxic effect on the LNCaP cell line. Different types of cell death were induced by the tested compounds. Apoptosis was induced by quercetin, while autophagy-specific changes were observed after using CAPE. Compounds obtained from other bee products have antiproliferative and cytotoxic activity against LNCaP prostate cancer cells. MDPI 2023-07-28 /pmc/articles/PMC10420981/ /pubmed/37570691 http://dx.doi.org/10.3390/molecules28155719 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woźniak, Przemysław
Kleczka, Anna
Jasik, Krzysztof
Kabała-Dzik, Agata
Dzik, Radosław
Stojko, Jerzy
The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title_full The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title_fullStr The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title_full_unstemmed The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title_short The Effect of Natural Substances Contained in Bee Products on Prostate Cancer in In Vitro Studies
title_sort effect of natural substances contained in bee products on prostate cancer in in vitro studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420981/
https://www.ncbi.nlm.nih.gov/pubmed/37570691
http://dx.doi.org/10.3390/molecules28155719
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