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Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells

This study aims to investigate the protective effects and mechanisms of pectolinarin against oxidative stress-induced cell damage in SH-SY5Y cells. Neurodegenerative diseases—such as Alzheimer’s disease—are potentially associated with oxidative stress, which causes excessive production of reactive o...

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Autores principales: Pang, Qi Qi, Kim, Ji Hyun, Kim, Hyun Young, Kim, Ji-Hyun, Cho, Eun Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420989/
https://www.ncbi.nlm.nih.gov/pubmed/37570795
http://dx.doi.org/10.3390/molecules28155826
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author Pang, Qi Qi
Kim, Ji Hyun
Kim, Hyun Young
Kim, Ji-Hyun
Cho, Eun Ju
author_facet Pang, Qi Qi
Kim, Ji Hyun
Kim, Hyun Young
Kim, Ji-Hyun
Cho, Eun Ju
author_sort Pang, Qi Qi
collection PubMed
description This study aims to investigate the protective effects and mechanisms of pectolinarin against oxidative stress-induced cell damage in SH-SY5Y cells. Neurodegenerative diseases—such as Alzheimer’s disease—are potentially associated with oxidative stress, which causes excessive production of reactive oxygen species (ROS) that damage DNA and proteins in neuronal cells. The results of this study demonstrate that pectolinarin can scavenge hydroxyl and nitric oxide radicals in a concentration-dependent manner. Moreover, pectolinarin significantly increased cell viability while reducing ROS production and LDH release in the hydrogen peroxide (H(2)O(2))-induced control group. Additionally, Pectolinarin recovered protein expression from H(2)O(2)-altered levels back to close-to-normal SH-SY5Y cell levels for components of the oxidative stress, inflammation, and apoptosis pathways—such as nuclear factor erythroid 2-related factor 2 (Nrf2), kelch-like ECH-associated protein (Keap1), anti-heme oxygenase 1 (HO-1), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), B-cell lympho-ma-2 (Bcl-2) protein, and Bcl-2-associated X protein (Bax). These findings suggest that pectolinarin has the potential to be used as a plant material for functional foods to be applied in the treatment of neurodegenerative diseases, such as Alzheimer’s disease, by mitigating oxidative stress-induced damage to neuronal cells.
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spelling pubmed-104209892023-08-12 Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells Pang, Qi Qi Kim, Ji Hyun Kim, Hyun Young Kim, Ji-Hyun Cho, Eun Ju Molecules Article This study aims to investigate the protective effects and mechanisms of pectolinarin against oxidative stress-induced cell damage in SH-SY5Y cells. Neurodegenerative diseases—such as Alzheimer’s disease—are potentially associated with oxidative stress, which causes excessive production of reactive oxygen species (ROS) that damage DNA and proteins in neuronal cells. The results of this study demonstrate that pectolinarin can scavenge hydroxyl and nitric oxide radicals in a concentration-dependent manner. Moreover, pectolinarin significantly increased cell viability while reducing ROS production and LDH release in the hydrogen peroxide (H(2)O(2))-induced control group. Additionally, Pectolinarin recovered protein expression from H(2)O(2)-altered levels back to close-to-normal SH-SY5Y cell levels for components of the oxidative stress, inflammation, and apoptosis pathways—such as nuclear factor erythroid 2-related factor 2 (Nrf2), kelch-like ECH-associated protein (Keap1), anti-heme oxygenase 1 (HO-1), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), B-cell lympho-ma-2 (Bcl-2) protein, and Bcl-2-associated X protein (Bax). These findings suggest that pectolinarin has the potential to be used as a plant material for functional foods to be applied in the treatment of neurodegenerative diseases, such as Alzheimer’s disease, by mitigating oxidative stress-induced damage to neuronal cells. MDPI 2023-08-02 /pmc/articles/PMC10420989/ /pubmed/37570795 http://dx.doi.org/10.3390/molecules28155826 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pang, Qi Qi
Kim, Ji Hyun
Kim, Hyun Young
Kim, Ji-Hyun
Cho, Eun Ju
Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title_full Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title_fullStr Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title_full_unstemmed Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title_short Protective Effects and Mechanisms of Pectolinarin against H(2)O(2)-Induced Oxidative Stress in SH-SY5Y Neuronal Cells
title_sort protective effects and mechanisms of pectolinarin against h(2)o(2)-induced oxidative stress in sh-sy5y neuronal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420989/
https://www.ncbi.nlm.nih.gov/pubmed/37570795
http://dx.doi.org/10.3390/molecules28155826
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