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Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening

Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formatio...

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Autores principales: Wei, Feng, Wan, Ru, Shi, Zhigang, Ma, Wenli, Wang, Hao, Chen, Yongwei, Bo, Jianhua, Li, Yunxiang, An, Wei, Qin, Ken, Cao, Youlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421014/
https://www.ncbi.nlm.nih.gov/pubmed/37570945
http://dx.doi.org/10.3390/plants12152791
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author Wei, Feng
Wan, Ru
Shi, Zhigang
Ma, Wenli
Wang, Hao
Chen, Yongwei
Bo, Jianhua
Li, Yunxiang
An, Wei
Qin, Ken
Cao, Youlong
author_facet Wei, Feng
Wan, Ru
Shi, Zhigang
Ma, Wenli
Wang, Hao
Chen, Yongwei
Bo, Jianhua
Li, Yunxiang
An, Wei
Qin, Ken
Cao, Youlong
author_sort Wei, Feng
collection PubMed
description Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formation differences in N1 (red fruit) and N1Y (yellow fruit) at three stages of ripening. Twenty-seven carotenoids were identified in N1 and N1Y fruits during the M1, M2, and M3 periods, with the M2 and M3 periods being critical for the difference in carotenoid and color between N1 and N1Y fruit. Weighted gene co-expression network analysis (WGCNA), gene trend analysis, and correlation analysis suggest that PSY1 and ZDS16 may be important players in the synthesis of carotenoids during goji fruit ripening. Meanwhile, 63 transcription factors (TFs) were identified related to goji fruit carotenoid biosynthesis. Among them, four TFs (CMB1-1, WRKY22-1, WRKY22-3, and RAP2-13-like) may have potential regulatory relationships with PSY1 and ZDS16. This work sheds light on the molecular network of carotenoid synthesis and explains the differences in carotenoid accumulation in different colored goji fruits.
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spelling pubmed-104210142023-08-12 Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening Wei, Feng Wan, Ru Shi, Zhigang Ma, Wenli Wang, Hao Chen, Yongwei Bo, Jianhua Li, Yunxiang An, Wei Qin, Ken Cao, Youlong Plants (Basel) Article Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formation differences in N1 (red fruit) and N1Y (yellow fruit) at three stages of ripening. Twenty-seven carotenoids were identified in N1 and N1Y fruits during the M1, M2, and M3 periods, with the M2 and M3 periods being critical for the difference in carotenoid and color between N1 and N1Y fruit. Weighted gene co-expression network analysis (WGCNA), gene trend analysis, and correlation analysis suggest that PSY1 and ZDS16 may be important players in the synthesis of carotenoids during goji fruit ripening. Meanwhile, 63 transcription factors (TFs) were identified related to goji fruit carotenoid biosynthesis. Among them, four TFs (CMB1-1, WRKY22-1, WRKY22-3, and RAP2-13-like) may have potential regulatory relationships with PSY1 and ZDS16. This work sheds light on the molecular network of carotenoid synthesis and explains the differences in carotenoid accumulation in different colored goji fruits. MDPI 2023-07-27 /pmc/articles/PMC10421014/ /pubmed/37570945 http://dx.doi.org/10.3390/plants12152791 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Feng
Wan, Ru
Shi, Zhigang
Ma, Wenli
Wang, Hao
Chen, Yongwei
Bo, Jianhua
Li, Yunxiang
An, Wei
Qin, Ken
Cao, Youlong
Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title_full Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title_fullStr Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title_full_unstemmed Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title_short Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
title_sort transcriptomics and metabolomics reveal the critical genes of carotenoid biosynthesis and color formation of goji (lycium barbarum l.) fruit ripening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421014/
https://www.ncbi.nlm.nih.gov/pubmed/37570945
http://dx.doi.org/10.3390/plants12152791
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