Cargando…
Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening
Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formatio...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421014/ https://www.ncbi.nlm.nih.gov/pubmed/37570945 http://dx.doi.org/10.3390/plants12152791 |
_version_ | 1785088860410085376 |
---|---|
author | Wei, Feng Wan, Ru Shi, Zhigang Ma, Wenli Wang, Hao Chen, Yongwei Bo, Jianhua Li, Yunxiang An, Wei Qin, Ken Cao, Youlong |
author_facet | Wei, Feng Wan, Ru Shi, Zhigang Ma, Wenli Wang, Hao Chen, Yongwei Bo, Jianhua Li, Yunxiang An, Wei Qin, Ken Cao, Youlong |
author_sort | Wei, Feng |
collection | PubMed |
description | Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formation differences in N1 (red fruit) and N1Y (yellow fruit) at three stages of ripening. Twenty-seven carotenoids were identified in N1 and N1Y fruits during the M1, M2, and M3 periods, with the M2 and M3 periods being critical for the difference in carotenoid and color between N1 and N1Y fruit. Weighted gene co-expression network analysis (WGCNA), gene trend analysis, and correlation analysis suggest that PSY1 and ZDS16 may be important players in the synthesis of carotenoids during goji fruit ripening. Meanwhile, 63 transcription factors (TFs) were identified related to goji fruit carotenoid biosynthesis. Among them, four TFs (CMB1-1, WRKY22-1, WRKY22-3, and RAP2-13-like) may have potential regulatory relationships with PSY1 and ZDS16. This work sheds light on the molecular network of carotenoid synthesis and explains the differences in carotenoid accumulation in different colored goji fruits. |
format | Online Article Text |
id | pubmed-10421014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104210142023-08-12 Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening Wei, Feng Wan, Ru Shi, Zhigang Ma, Wenli Wang, Hao Chen, Yongwei Bo, Jianhua Li, Yunxiang An, Wei Qin, Ken Cao, Youlong Plants (Basel) Article Carotenoids in goji (Lycium barbarum L.) have excellent health benefits, but the underlying mechanism of carotenoid synthesis and color formation in goji fruit ripening is still unclear. The present study uses transcriptomics and metabolomics to investigate carotenoid biosynthesis and color formation differences in N1 (red fruit) and N1Y (yellow fruit) at three stages of ripening. Twenty-seven carotenoids were identified in N1 and N1Y fruits during the M1, M2, and M3 periods, with the M2 and M3 periods being critical for the difference in carotenoid and color between N1 and N1Y fruit. Weighted gene co-expression network analysis (WGCNA), gene trend analysis, and correlation analysis suggest that PSY1 and ZDS16 may be important players in the synthesis of carotenoids during goji fruit ripening. Meanwhile, 63 transcription factors (TFs) were identified related to goji fruit carotenoid biosynthesis. Among them, four TFs (CMB1-1, WRKY22-1, WRKY22-3, and RAP2-13-like) may have potential regulatory relationships with PSY1 and ZDS16. This work sheds light on the molecular network of carotenoid synthesis and explains the differences in carotenoid accumulation in different colored goji fruits. MDPI 2023-07-27 /pmc/articles/PMC10421014/ /pubmed/37570945 http://dx.doi.org/10.3390/plants12152791 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wei, Feng Wan, Ru Shi, Zhigang Ma, Wenli Wang, Hao Chen, Yongwei Bo, Jianhua Li, Yunxiang An, Wei Qin, Ken Cao, Youlong Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title | Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title_full | Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title_fullStr | Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title_full_unstemmed | Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title_short | Transcriptomics and Metabolomics Reveal the Critical Genes of Carotenoid Biosynthesis and Color Formation of Goji (Lycium barbarum L.) Fruit Ripening |
title_sort | transcriptomics and metabolomics reveal the critical genes of carotenoid biosynthesis and color formation of goji (lycium barbarum l.) fruit ripening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421014/ https://www.ncbi.nlm.nih.gov/pubmed/37570945 http://dx.doi.org/10.3390/plants12152791 |
work_keys_str_mv | AT weifeng transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT wanru transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT shizhigang transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT mawenli transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT wanghao transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT chenyongwei transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT bojianhua transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT liyunxiang transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT anwei transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT qinken transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening AT caoyoulong transcriptomicsandmetabolomicsrevealthecriticalgenesofcarotenoidbiosynthesisandcolorformationofgojilyciumbarbarumlfruitripening |