Cargando…

MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress

The toxicity of aluminum (Al) in acidic soils poses a significant limitation to crop productivity. In this study, we found a notable increase in DnaJ (HSP40) expression in the roots of Al-tolerant alfalfa (WL-525HQ), which we named MsDjB4. Transient conversion assays of tobacco leaf epidermal cells...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Siyan, Mo, Xin, Sun, Linjie, Gao, Li, Su, Liantai, An, Yuan, Zhou, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421020/
https://www.ncbi.nlm.nih.gov/pubmed/37570962
http://dx.doi.org/10.3390/plants12152808
_version_ 1785088862000775168
author Liu, Siyan
Mo, Xin
Sun, Linjie
Gao, Li
Su, Liantai
An, Yuan
Zhou, Peng
author_facet Liu, Siyan
Mo, Xin
Sun, Linjie
Gao, Li
Su, Liantai
An, Yuan
Zhou, Peng
author_sort Liu, Siyan
collection PubMed
description The toxicity of aluminum (Al) in acidic soils poses a significant limitation to crop productivity. In this study, we found a notable increase in DnaJ (HSP40) expression in the roots of Al-tolerant alfalfa (WL-525HQ), which we named MsDjB4. Transient conversion assays of tobacco leaf epidermal cells showed that MsDjB4 was targeted to the membrane system including Endoplasmic Reticulum (ER), Golgi, and plasma membrane. We overexpressed (MsDjB4-OE) and suppressed (MsDjB4-RNAi) MsDjB4 in alfalfa hairy roots and found that MsDjB4-OE lines exhibited significantly better tolerance to Al stress compared to wild-type and RNAi hairy roots. Specifically, MsDjB4-OE lines had longer root length, more lateral roots, and lower Al content compared to wild-type and RNAi lines. Furthermore, MsDjB4-OE lines showed lower levels of lipid peroxidation and ROS, as well as higher activity of antioxidant enzymes SOD, CAT, and POD compared to wild-type and RNAi lines under Al stress. Moreover, MsDjB4-OE lines had higher soluble protein content compared to wild-type and RNAi lines after Al treatment. These findings provide evidence that MsDjB4 contributes to the improved tolerance of alfalfa to Al stress by facilitating protein synthesis and enhancing antioxidant capacity.
format Online
Article
Text
id pubmed-10421020
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104210202023-08-12 MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress Liu, Siyan Mo, Xin Sun, Linjie Gao, Li Su, Liantai An, Yuan Zhou, Peng Plants (Basel) Article The toxicity of aluminum (Al) in acidic soils poses a significant limitation to crop productivity. In this study, we found a notable increase in DnaJ (HSP40) expression in the roots of Al-tolerant alfalfa (WL-525HQ), which we named MsDjB4. Transient conversion assays of tobacco leaf epidermal cells showed that MsDjB4 was targeted to the membrane system including Endoplasmic Reticulum (ER), Golgi, and plasma membrane. We overexpressed (MsDjB4-OE) and suppressed (MsDjB4-RNAi) MsDjB4 in alfalfa hairy roots and found that MsDjB4-OE lines exhibited significantly better tolerance to Al stress compared to wild-type and RNAi hairy roots. Specifically, MsDjB4-OE lines had longer root length, more lateral roots, and lower Al content compared to wild-type and RNAi lines. Furthermore, MsDjB4-OE lines showed lower levels of lipid peroxidation and ROS, as well as higher activity of antioxidant enzymes SOD, CAT, and POD compared to wild-type and RNAi lines under Al stress. Moreover, MsDjB4-OE lines had higher soluble protein content compared to wild-type and RNAi lines after Al treatment. These findings provide evidence that MsDjB4 contributes to the improved tolerance of alfalfa to Al stress by facilitating protein synthesis and enhancing antioxidant capacity. MDPI 2023-07-28 /pmc/articles/PMC10421020/ /pubmed/37570962 http://dx.doi.org/10.3390/plants12152808 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Siyan
Mo, Xin
Sun, Linjie
Gao, Li
Su, Liantai
An, Yuan
Zhou, Peng
MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title_full MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title_fullStr MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title_full_unstemmed MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title_short MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
title_sort msdjb4, a hsp40 chaperone in alfalfa (medicago sativa l.), improves alfalfa hairy root tolerance to aluminum stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421020/
https://www.ncbi.nlm.nih.gov/pubmed/37570962
http://dx.doi.org/10.3390/plants12152808
work_keys_str_mv AT liusiyan msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT moxin msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT sunlinjie msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT gaoli msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT suliantai msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT anyuan msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress
AT zhoupeng msdjb4ahsp40chaperoneinalfalfamedicagosativalimprovesalfalfahairyroottolerancetoaluminumstress